LIX1L aggravates MASH-HCC progression by reprogramming of hepatic metabolism and microenvironment via CD36.

Limb expression 1-like protein (LIX1L) is an essential player in liver disorders, but its function in metabolic dysfunction-associated steatohepatitis (MASH) and associated hepatocellular carcinoma (HCC) progression remains obscure. Here, we identify LIX1L as a key integrative regulator linking lipid metabolism and inflammation, adipose tissue and hepatic microenvironment, which promotes MASH progression. LIX1L significantly upregulates in MASH patients, mouse models, and palmitic acid-stimulated hepatocytes.

Sampling efficiency of flow-through air sampler: Effect of sampling rate.

The Flow Through Sampler (FTS) serves as an efficient wind-powered active sampling device, enabling the collection of a significant air volume within a short duration. Throughout the actual sampling process, the sampling rate of FTS experiences continuous fluctuations, highlighting the need for in-depth investigation into its impact on breakthrough. In this study, we interconnected the FTS sampling column with three distinct pumps, operating at five varied sampling rates under 293 K and 303 K; and breakthrough profiles of PCBs, PAHs and OCPs on the FTS sampling column were investigated to understand the impacts of sampling rate, expressed as linear wind velocity through the cross section of FTS tube (u) on the sampling efficiency of the sampling column.

Intratumoral injection of mRNA encoding survivin in combination with STAT3 inhibitor stattic enhances antitumor effects.

Intratumoral delivery of mRNA encoding immunostimulatory molecules can initiate a robust, global antitumor response with little side effects by enhancing local antigen presentation in the tumor and the tumor draining lymph node. Neoantigen-based mRNA nanovaccine can inhibit melanoma growth in mice by intratumoral injection. Myeloid-derived suppressor cells (MDSCs) suppress antitumor immune responses by secreting immunosuppressive agents, such as reactive oxygen species (ROS).

Lefamulin Overcomes Acquired Drug Resistance via Regulating Mitochondrial Homeostasis by Targeting ILF3 in Hepatocellular Carcinoma.

Acquired resistance represents a critical clinical challenge to molecular targeted therapies such as tyrosine kinase inhibitors (TKIs) treatment in hepatocellular carcinoma (HCC). Therefore, it is urgent to explore new mechanisms and therapeutics that can overcome or delay resistance. Here, a US Food and Drug Administration (FDA)-approved pleuromutilin antibiotic is identified that overcomes sorafenib resistance in HCC cell lines, cell line-derived xenograft (CDX) and hydrodynamic injection mouse models.

Differential expression and clinical significance of IGF2BP3 in peritoneal dialysate of patients with varying duration of peritoneal dialysis.

This study aims to investigate the differential expression of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in the peritoneal dialysate among patients with different durations of peritoneal dialysis and its association with the angiogenic marker vascular* endothelial growth factor (VEGF), the fibronectin (FN), and various clinical indicators. A cohort of 122 peritoneal dialysis patients was categorized into short-term (≤1 year, n = 33), mid-term (>1 and ≤5 years, n = 55), and long-term (>5 years, n = 34) groups based on dialysis duration. We utilized enzyme-linked immunosorbent assay (ELISA) and western blot assays to quantify the levels of IGF2BP3, VEGF, and FN in the dialysate.

Silver-palladium bimetallic nanoparticles stabilized by elm pod polysaccharide with peroxidase-like properties for glutathione detection and photothermal anti-tumor ability.

Noble metal nanoparticles show good application prospects in biosensors and anti-tumor drug research. Herein, the near-spherical silver‑palladium bimetallic nanoparticles supported by elm pod polysaccharide (EPP-AgPd NPs) were prepared by using the elm pod polysaccharide (EPP). EPP acts as a stabilizer and reducing agent due to its water solubility and weak reducing ability.

DAU-Net: A medical image segmentation network combining the Hadamard product and dual scale attention gate.

Medical image segmentation has an important application value in the modern medical field, it can help doctors accurately locate and analyze the tissue structure, lesion areas, and organ boundaries in the image, which provides key information support for clinical diagnosis and treatment, but there are still a large number of problems in the accuracy of the segmentation, so in this paper, we propose a medical image segmentation network combining the Hadamard product and dual-scale attention gate (DAU-Net). First, the Hadamard product is introduced in the structure of the fifth layer of the codec for element-by-element multiplication, which can generate feature representations with more representational capabilities. Second, in the jump connection module, we propose a dual scale attention gating (DSAG), which can highlight more valuable features and achieve more efficient jump connections.

Sampling efficiency of a polyurethane foam air sampler: Effect of temperature.

Effective monitoring of atmospheric concentrations is vital for assessing the Stockholm Convention's effectiveness on persistent organic pollutants (POPs). This task, particularly challenging in polar regions due to low air concentrations and temperature fluctuations, requires robust sampling techniques. Furthermore, the influence of temperature on the sampling efficiency of polyurethane foam discs remains unclear.

Classical thiazole orange and its regioisomer as fluorogenic probes for nucleolar RNA imaging in living cells.

In contrast to well-established DNA-selective dyes for live cell imaging, RNA-selective dyes have been less developed owing to the challenges of making small molecules have RNA selectivity over DNA. Two kinds of dyes are now commercially available for nucleolar RNA imaging in cells, but these two dyes do not apply to living cells and have limited use in fixed and permeabilized cells. Herein, we report on thiazole orange (TO), a well-known nucleic acid stain, as a promising fluorogenic dye for nucleolar RNA imaging in living cells.

STAT3 signaling mediates peritoneal fibrosis by activating hyperglycolysis.

Background: Long term peritoneal dialysis leads to peritoneal epithelial-mesenchymal transformation (EMT), angiogenesis, and ultrafiltration failure. Although recent evidence suggests that inhibiting STAT3 (signal transducer and activator of transcription 3) can prevent kidney fibrosis, and that STAT3 can enhance glucose metabolism, the effect of STAT3 in peritoneal fibrosis (PF) has not been clarified.

Methods: Our study determined the effects of STAT3 on EMT and key glycolysis enzymes in mesothelial HMrSV5 cells by knockdown and overexpression of STAT3.

Green synthesis of Ag nanoparticles using elm pod polysaccharide for catalysis and bacteriostasis.

The green synthesis of silver nanoparticles (Ag NPs) for catalysis and biological applications has gained great interest. Natural elm pods are a type of food that possesses anti-inflammatory and pain-relieving effects. In this study, elm pod polysaccharide (EPP) was extracted from elm pods using hot water extraction for the first time.

Digital inclusive finance and asset allocation of Chinese residents: Evidence from the China Household Finance Survey.

Combined with the expected utility theory, this paper constructs a theoretical analysis framework including the development level, financial literacy, and intelligence level of Inclusive Finance, puts forward the hypothesis of the development of digital Inclusive Finance on household asset allocation, and uses the data of China's household finance survey to verify the theory proposed in this paper. The empirical results show that: (1) digital inclusive Finance can significantly improve the allocation proportion of household risk assets, promote the rational participation of households in the risk financial market, and improve the allocation efficiency of household resources. (2) Digital inclusive finance can significantly improve the income level of family financial investment and optimize family investment decision-making.

[Source Analysis and Composition Characteristics of Water-soluble Ions During Spring Festival in Ningbo].

Water-soluble ions and some trace gases during the Spring Festival in Ningbo were observed using an ion online gas composition and aerosol monitoring system. Combined with meteorological elements data, the source analysis and composition characteristics of water-soluble ions and trace gases were analyzed. The average concentration of (PM) during the observation period was 33.

Quantitative Evaluation of Gait Changes Using APDM Inertial Sensors After the External Lumbar Drain in Patients With Idiopathic Normal Pressure Hydrocephalus.

Gait and balance disturbances are common symptoms of idiopathic normal pressure hydrocephalus (iNPH). This study aimed to quantitatively evaluate gait and balance parameters after external lumbar drainage (ELD) using APDM inertial sensors. Two-minute walkway tests were performed in 36 patients with suspected iNPH and 20 healthy controls.

Doxorubicin-loaded natural daptomycin micelles with enhanced targeting and anti-tumor effect in vivo.

Development of a simple method to enhance targeting and anti-tumor effect of the chemotherapeutic agents in vivo is a major problem. Amphipathic and natural daptomycin is biocompatible antibacterial polypeptide used in clinical practice. Herein, doxorubicin (DOX) was stabilized by zwitterionic daptomycin (Dap) micelles in aqueous solution to form a zwitterionic nanodrug (Dap-DOX micelles).

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid.

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with KPtCl.

Reversing P-Glycoprotein-Associated Multidrug Resistance of Breast Cancer by Targeted Acid-Cleavable Polysaccharide Nanoparticles with Lapatinib Sensitization.

For reversing the treatment failure in P-glycoprotein (P-gp)-associated MDR (multidrug resistance) of breast cancer, a high dose of Lapatinib (Lap), a substrate of breast cancer-resistant protein, was encapsulated into safe and effective acid-cleavable polysaccharide-doxorubicin (Dox) conjugates to form targeted HPP-Dox/Lap nanoparticles with an optimal drug ratio and appropriate nanosize decorated with oligomeric hyaluronic acid (HA) for specially targeting overexpressed CD44 receptors of MCF-7/ADR. The markedly increased cellular uptake and the strongest synergetic cytotoxicity revealed the enhanced reversal efficiency of HPP-Dox/Lap nanoparticles with reversal multiples at 29.83.

Atmospheric fine particles in a typical coastal port of Yangtze River Delta.

In recent decades, coastal ports have experienced rapid development and become an important economic and ecological hub in China. Atmospheric particle is a research hotspot in atmospheric environmental sciences in inland regions. However, few studies on the atmospheric particle were conducted in coastal port areas in China, which indeed suffers atmospheric particle pollution.

Practical Computation of FFT-based Generalized Two-dimensional Correlation Spectroscopy.

A practical computation of fast Fourier transformation (FFT) based generalized two-dimensional (2D) correlation spectroscopy is described. Using simple sinusoids, we tested and confirmed that the method served effectively and properly, invariant to the changes of the number of data points of the time profiles. This computation is applicable to any type of waveforms in a versatile manner.

Nickel-Catalyzed Reductive Coupling for Transforming Unactivated Aryl Electrophiles into β-Fluoroethylarenes.

We report herein a facile synthetic method for converting unactivated (hetero)aryl electrophiles into β-fluoroethylated (hetero)arenes via nickel-catalyzed reductive cross-couplings. This coupling reaction features the involvement of FCH CH radical intermediate rather than β-fluoroethyl manganese species which provides effective solutions to the problematic β-fluoride side eliminations. The practical value of this protocol is further demonstrated by the late-stage modification of several complex ArCl or ArOH-derived bioactive molecules.

Seasonal variation and size distributions of water-soluble inorganic ions and carbonaceous aerosols at a coastal site in Ningbo, China.

Size-fractioned aerosol samples were collected by an eight-stage Anderson sampler for four seasons from November 2014 to August 2015 at a coastal and suburban site in Ningbo, China, with a total of 270 samples were obtained. The seasonal variations and size distributions of water-soluble inorganic ions (WSIIs), carbonaceous aerosols (OC and EC), which consist of four organic carbon (OC1-OC4), pyrolyzed carbon (OP) and three elemental carbon fraction (EC1-EC3), were investigated. For the sampling periods, the average total concentration of WSIIs, OC and EC in PM, PM and PM were 21.

Effect of intermittent operation model on the function of soil infiltration system.

To enhance denitrification in a process of solute infiltration through a soil, a two-section mixed-medium soil infiltration system (TMSIS) for urban non-point pollution was developed. The artificial aerobic respiration and nitrification took place in the upper aerobic section (AES), while grass powders and sawdust were mixed in the bottom anaerobic section (ANS) to supply organic carbon source for denitrification bacteria, and the reduction was increased by iron addition in the ANS. Measured resident concentrations from the bottom of each ANS column were assumed to represent mean values averaged over the column cross-sectional area.

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Reactive oxygen species (ROS) have emerged as important signaling molecules that play crucial roles in carcinogenesis and cytotoxic responses. Nrf2 is the master regulator of ROS balance. Thus, uncovering mechanisms of Nrf2 regulation is important for the development of alternative treatment strategies for cancers.

Localized multidrug co-delivery by injectable self-crosslinking hydrogel for synergistic combinational chemotherapy.

Significant progress has been made in the use of injectable hydrogels as drug carrier systems to treat cancers by the peritumoral-localized co-delivery of multiple drugs with different therapeutic mechanisms. In this study, a novel, injectable self-crosslinking HA-SH hydrogel was able to concurrently encapsulate multiple drugs (sorafenib, doxorubicin, and metformin) to enhance chemotherapy efficacy. The hydrogel was relatively stable under physiological conditions and could quickly and directly release the loaded drugs to the tumor site in a reductive tumor microenvironment.

Correction: Design, synthesis, crystal structure and fungicidal activity of ()-5-(methoxyimino)-3,5-dihydrobenzo[][1,2]oxazepin-4(1)-one analogues.

[This corrects the article DOI: 10.1039/C7MD00025A.].