Isolation and characterization of phosphoglycerate kinase and creatine kinase from bighead carp (Aristichthys nobilis): Potential sources for antitumor agents.

Bighead carp (Aristichthys nobilis) has garnered significant attention due to its potential health benefits, yet its bioactive protein components remain largely unexplored. In this study, two proteins S3 and Z1 were isolated from Aristichthys nobilis using ammonium sulfate precipitation and serial column chromatography guided by their in vitro antitumor activity. Both proteins were found to be homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a purity exceeding 95% as confirmed by reverse-phase high performance liquid chromatography (RP-HPLC).