[Optimization for determination methods of alpha-, beta- carotene in vegetables].

Objective: To optimize the determination method of carotene in order to separate the alpha and beta carotene.

Methods: The homogenized samples were extracted with ethanol before saponification, and then extracted with petroleum ether followed by wash, dry and concentration. At last, the separation was performed on the high performance liquid chromatographic with YMC Carotenoid C30 column (3 microm 4.