Isolation and characterization of a from fish pond water.

Introduction: The intricate habitats of aquatic organisms, coupled with the prevalence of pathogens, contribute to a high incidence of various diseases, particularly bacterial infections. Consequently, the formulation of sustainable and effective disease management strategies is crucial for the thriving aquaculture sector.

Methods And Results: In this investigation, a strain of , designated , was isolated from a freshwater fish pond.

Efficient and low resistance multi-functional UV resistant polyphenylene sulfide non-woven hollow membrane.

The heat and corrosion resistance of traditional membranes is inadequate, thus making them inadequate for the separation/filtration needs of harsh environments. Polyphenylene sulfide(PPS) can be used to develop new-generation membrane materials, but PPS has problems such as hydrophobicity and UV resistance. This article proposes a PPS membrane for efficient separation/filtration under extreme conditions, which uses melt-blown PPS non-woven fabric and undergoes oxidation and nitrification modification.

A Peritrophin-44 gene in Litopenaeus vannamei is involved in disease resistance.

Peritrophic membrane (PM) is a pellicle structure present in the midgut of some invertebrates, such as insects and crustaceans. It could isolate harmful components and pathogens in food from intestinal epithelial cells; and it also plays a role in improving digestion and absorption efficiency. So PM is important for survival of its owner.

Factors associated with burnout among frontline nurses in the post-COVID-19 epidemic era: a multicenter cross-sectional study.

Background: The COVID-19 pandemic has significantly increased the risk of burnout among frontline nurses. However, the prevalence of burnout and its associated factors in the post-pandemic era remain unclear. This research aims to investigate burnout prevalence among frontline nurses in the post-pandemic period and pinpoint associated determinants in China.

Identification of potential crucial genes and therapeutic targets for epilepsy.

Background: Epilepsy, a central neurological disorder, has a complex genetic architecture. There is some evidence suggesting that genetic factors play a role in both the occurrence of epilepsy and its treatment. However, the genetic determinants of epilepsy are largely unknown.

Integrative analysis of rs717620 polymorphism in therapeutic response to anti-seizure medications.

Background: Previous studies have shown that the rs717620 polymorphism in , the gene encoding multidrug resistance protein 2, influences the therapeutic response to anti-seizure medications (ASMs). However, this result is not consistent, and the mechanism by which rs717620 influences ASM responses is unclear.

Aims: The present study evaluated the association between rs717620 genotype and ASM efficacy, and examined the potential mechanisms.

Comprehensive analyses identify potential biomarkers for encephalitis in HIV infection.

Human immunodeficiency virus encephalitis (HIVE) is a severe neurological complication after HIV infection. Evidence shows that genetic factors play an important role in HIVE. The aim of the present study was to identify new potential therapeutic targets for HIVE.

Functional Characterization of A Deformed Epidermal Autoregulatory Factor 1 Gene in Litopenaeus vannamei.

Innate immunity is crucial for invertebrate defense against pathogenic infections. Numerous studies have indicated that the Toll-NF-κB pathway plays an important role in this process, particularly in anti-bacterial and anti-fungal immunity. Although the function of this pathway has been studied extensively, there are still uncertainties regarding its role in shrimp.

A Study on the Structural Relationships between COVID-19 Coping Strategies, Positive Expectations, and the Behavioral Intentions of Various Tourism-Related Behaviors.

The purpose of this study was to investigate the impact of coping strategies, attitudes, and positive anticipated emotions on the positive expectations and behavioral intentions of Korean tourists during the COVID-19 pandemic. An integrated model was proposed and tested, and the results indicate that effective coping strategies, attitudes, and positive anticipated emotions have a positive effect on the positive expectations of tourism during the pandemic, which in turn positively influences behavioral intentions. Practical suggestions were also provided based on the findings.

Polyphenylene Sulfide Ultrafine Viscous Fibrous Membrane Modified by ZIF-8 for Highly Effective Oil/Water Separation under High Salt or Alkaline Conditions.

The oil/water separation in harsh environments has always been a challenging topic all over the world. In this study, the ZIF-8/PPS fiber membranes were fabricated via the combination of hot pressing and in situ growth. The distribution of ZIF-8 in the membranes was adjusted by changing the ZIF-8 in situ growth time, which could control the oil/water separation effect.

Integrative Analyses Identify KCNJ15 as a Candidate Gene in Patients with Epilepsy.

Introduction: Although there is accumulating evidence that genetic factors play a vital role in the pathogenesis of epilepsy, few epilepsy-associated genes have been identified. Additionally, the role of KCNJ15 in epilepsy has not been evaluated so far.

Methods: Here, we performed differentially expressed gene analysis, expression quantitative trait loci analysis, gene co-expression analysis, and protein-protein interaction analysis to evaluate the role of KCNJ15 in epilepsy.

Cerebral infarction caused by systemic sclerosis: a case report.

Systemic sclerosis, also known as scleroderma, is a rare multisystem autoimmune disease characterized by vascular lesions caused by collagen deposition in the skin and viscera and damage to the endothelium. Endothelial injury and microvascular occlusion result in Raynaud's phenomenon, finger ischemia, pulmonary hypertension, and scleroderma renal crisis. Scleroderma itself is a rare disease with an incidence ranging from 0.

Skeletal Muscle Constructs Engineered from Human Embryonic Stem Cell Derived Myogenic Progenitors Exhibit Enhanced Contractile Forces When Differentiated in a Medium Containing EGM-2 Supplements.

Three-dimensional (3D) skeletal muscle constructs engineered from myogenic progenitors derived from human pluripotent stem cells (hPSCs) have a wide range of applications, but to date, such constructs generate lower specific tetanic force than adult human muscles. Methods enhancing functional muscle differentiation and force generation of these constructs are highly desirable. The finding of this study is that addition of the supplements in the endothelial cell growth medium-2 (EGM-2) to the myogenic differentiation medium can substantially enhance contractile force generation.

Efficient release of immunocaptured cells using coiled-coils in a microfluidic device.

Label-free and affinity-based cell separation allows highly specific cell capture through simple procedures, but it remains a major challenge to efficiently release the captured cells without changing their structure, phenotype, and function. We report a microfluidic platform for label-free immunocapture of target cells and efficient release of the cells with minimal biochemical and biophysical perturbations. The method capitalizes on self-assembly of a pair of heterodimerizing coiled-coils, A and B.

Oxygen-releasing polycaprolactone/calcium peroxide composite microspheres.

Oxygen-releasing polycaprolactone/calcium peroxide (PCL/CaO ) composite microspheres were fabricated via homogenization, electrospray with a single nozzle, and electrospray with a co-axial nozzle, resulting in homogenized, single-walled, and double-walled microspheres, respectively. Scanning electron microscopy revealed that homogenized microspheres had pores, while electrosprayed microspheres did not. Alizarin Red S staining showed a core-shell structure for double-walled microspheres.

Doxorubicin-Conjugated PAMAM Dendrimers for pH-Responsive Drug Release and Folic Acid-Targeted Cancer Therapy.

We present here the development of multifunctional doxorubicin (DOX)-conjugated poly(amidoamine) (PAMAM) dendrimers as a unique platform for pH-responsive drug release and targeted chemotherapy of cancer cells. In this work, we covalently conjugated DOX onto the periphery of partially acetylated and folic acid (FA)-modified generation 5 (G5) PAMAM dendrimers through a pH-sensitive -aconityl linkage to form the G5.NHAc-FA-DOX conjugates.

Efficient Release of Affinity-Captured Cells Using a Coiled-Coil-Based Molecular Trigger.

Affinity-based cell separation is label-free and highly specific, but it is difficult to efficiently and gently release affinity-captured cells due to the multivalent nature of cell-material interactions. To address this challenge, we have developed a platform composed of a capture substrate and a cell-releasing molecular trigger. The capture substrate is functionalized with a cell-capture antibody and a coiled-coil A.

Impact of dendrimer surface functional groups on the release of doxorubicin from dendrimer carriers.

Generation 5 (G5) poly(amidoamine) dendrimers with acetyl (G5.NHAc), glycidol hydroxyl (G5.NGlyOH), and succinamic acid (G5.

Multifunctional dendrimer/combretastatin A4 inclusion complexes enable in vitro targeted cancer therapy.

Background: We report here a unique approach to using multifunctional dendrimer/combretastatin A4 (CA4) inclusion complexes for targeted cancer therapeutics.

Methods: Amine-terminated generation 5 polyamidoamine dendrimers were first partially acetylated to neutralize a significant portion of the terminal amines, and then the remaining dendrimer terminal amines were sequentially modified with fluorescein isothiocyanate as an imaging agent and folic acid as a targeting ligand. The multifunctional dendrimers formed (G5.