Assessment of internal exposure risk from metals pollution of occupational and non-occupational populations around a non-ferrous metal smelting plant.

Non-ferrous metal smelting poses significant risks to public health. Specifically, the copper smelting process releases arsenic, a semi-volatile metalloid, which poses an emerging exposure risk to both workers and nearby residents. To comprehensively understand the internal exposure risks of metal(loid)s from copper smelting, we explored eighteen metal(loid)s and arsenic metabolites in the urine of both occupational and non-occupational populations using inductively coupled plasma mass spectrometry with high-performance liquid chromatography and compared their health risks.

Internal exposure risk based on urinary metabolites of PAHs of occupation and non-occupation populations around a non-ferrous metal smelting plant.

The emission of various metals from non-ferrous metal smelting activities is well known. However, relative investigations on potential occupational exposure of organic pollutants are still limited. Herein, total of 619 human urine samples were collected from workers engaged in smelting activities and residents living near and/or far from the smelting sites, and ten mono-hydroxylated metabolites of polycyclic aromatic hydrocarbons (OH-PAHs) in human urine were determined.

Pollution Profiles, Source Identification and Health Risk Assessment of Heavy Metals in Soil near a Non-Ferrous Metal Smelting Plant.

Heavy metal pollution related to non-ferrous metal smelting may pose a significant threat to human health. This study analyzed 58 surface soils collected from a representative non-ferrous metal smelting area to screen potentially hazardous heavy metals and evaluate their health risk in the studied area. The findings demonstrated that human activity had contributed to the pollution degrees of Cu, Cd, As, Zn, and Pb in the surrounding area of a non-ferrous metal smelting plant (NMSP).

Eosinophils may serve as CEA-secreting cells for allergic bronchopulmonary aspergillosis (ABPA) patients.

Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterized by an exaggerated response of the immune system to the fungus Aspergillus. This study aimed to assess the relationship between carcinoembryonic antigen (CEA) and eosinophils in ABPA patients. We describes a case of a 50-year-old patient who was diagnosed with ABPA presenting with high level of CEA and eosinophils.

[Cloning and functional identification of a new NADPH-cytochrome P450 reductase in Andrographis paniculata].

Andrographolide is a main active ingredient in traditional Chinese medicine Andrographis paniculata，with a variety of pharmacological activity，widely used in clinical practice. However its biosynthetic pathway has not been resolved. Cytochrome P450 reductase provides electrons for CYP450 and plays an important role in the CYP450 catalytic process.

Ibrutinib Exacerbates Bleomycin-Induced Pulmonary Fibrosis via Promoting Inflammation.

Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible lung disease with high mortality rate. The etiology is unknown and treatment choices are limited. Thus, there is great interest to investigate novel agents for IPF therapy.

[Overexpression and isolation of CYP76AH1 in Salvia miltiorrhiza hairy roots].

Protein complexes are involved in the synthesis of multiple secondary metabolites in plants, and their separation is essential to elucidate plant secondary metabolism and improve in vitro catalytic efficiency. In this study, the transgenic hairy roots of CYP76AH1, a key enzyme of tanshinone synthesis pathway, was constructed and the transgenic hairy roots of Danshen overexpressing CYP76AH1 protein were screened by Western blotting and used as a tissue culture material for the subsequent extraction of protein complex in tanshinone synthesis pathway. By optimizing the type and concentration of the detergent in the protein extraction buffer, the buffer containing 0.

[Cloning, subcellular localization, and heterologous expression of ApNAC1 gene from Andrographis paniculata].

Andrographis paniculata is widely used as medicinal herb in China for a long time and andrographolide is its main medicinal constituent. To investigate the underlying andrographolide biosynthesis mechanisms, RNA-seq for A. paniculata leaves with MeJA treatment was performed.

Molecular cloning and functional characterization of multiple NADPH-cytochrome P450 reductases from Andrographis paniculata.

Andrographis paniculata (Burm.f.) Wall.

[Effect of Chemical Constituents from Glycyrrhiza uralensis Induced by Exogenous 6-Benzylaminopurine in Different Growth Periods].

Objective: To study the effect of chemical constituents from Glycyrrhiza uralensis induced by exogenous 6-benzylaminopurine( 6-BA) in different growth periods, aim to explore the regulatory network of Glycyrrhiza uralensis.

Methods: The transplants of two-year-old Glycyrrhiza uralensis were subjected to four concentrations of 6-BA( 15,20,50,100 mg / L) at June and July. The content of seven kinds of chemical constituents including glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, liquiritin apioside,isoliquiritin apioside were determined by HPLC.

[Study on chemical constituents in Lysinotus wilsonii by UPLC-Q-TOF-MS].

The Ultra-high Performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry（UPLC-Q-TOF-MS）was applied to analyze the chemical components in Lysinotus wilsonii. A Waters ACQUITY UPLC-BEH-C₁₈ S column（2.1 mm×100 mm,1.

[Rapid analysis of compounds in leaves of Chinese seabuckthorn and Tibetan seabuckthorn by UPLC/Q-TOF-MS].

The study is aimed to analyze the chemical components in leaves of Chinese seabuckthorn and Tibetan seabuckthorn qualitatively and compare the differences between them by using ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS).The chromatographic separation of the components was achieved ona Waters ACQUITY UPLC-T3 column (2.1 mm×100 mm, 1.