Nanoencapsulation of Living Microbial Cells in Porous Covalent Organic Framework Shells.

Encapsulating living cells within nanoshells offers an important approach to enhance their stability against environmental stressors and broaden their application scope. However, this often leads to impaired mass transfer at the cell biointerface. Strengthening the protective shell with well-defined, ordered transport channels is crucial to regulating molecular transport and maintaining cell viability and biofunctionality.

Reticular Chemistry for Enhancing Bioentity Stability and Functional Performance.

Addressing the fragility of bioentities that results in instability and compromised performance during storage and applications, reticular chemistry, specifically through metal-organic frameworks (MOFs) and covalent organic frameworks (COFs), offers versatile platforms for stabilization and enhancement of bioentities. These highly porous frameworks facilitate efficient loading and mass transfer, offer confined environments and selective permeability for stabilization and protection, and enable finely tunable biointerfacial interactions and microenvironments for function optimization, significantly broadening the applications of various bioentities, including enzymes, nucleic acids, cells, etc. This Perspective outlines strategies for integrating bioentities with reticular frameworks, highlighting new design ideas for existing issues within these strategies.

Covalent Organic Framework Based Crosslinked Porous Microcapsules for Enzymatic Catalysis.

The design of porous microcapsules with selective mass transfer and mechanical robustness for enzyme encapsulation is highly desired for biocatalysis, yet the construction remains challenging. Herein, we report the facile fabrication of porous microcapsules by assembling covalent organic framework (COF) spheres at the interfaces of emulsion droplets followed by interparticle crosslinking. The COF microcapsules could offer an enclosed aqueous environment for enzymes, with size-selective porous shells that allow for the fast diffusion of substrates and products while excluding larger molecules such as protease.