Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in Enteritidis.

Two-component systems (TCS) of serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six . Enteritidis RR-encoding gene deletion mutants.

Effects of cropland-to-orchard conversion on soil multifunctionality, particularly nitrogen cycling in the eastern Loess Plateau.

The conversion of cropland to orchards is one of the main measures of the Grain for Green Program for soil and water conservation and ecosystem function maintenance in the eastern Loess Plateau, China. However, the patterns and influencing forces of soil multifunctionality during the conversion from cropland to orchard remain unclear. This study evaluated the responses and regulating factors of soil multifunctionality following the conversion of cropland to pomegranate ( L.

Managing fruit rot diseases of .

Blueberry is an important perennial fruit crop with expanding consumption and production worldwide. Consumer demand for blueberries has grown due to the desirable flavor and numerous health benefits, and fresh market production in the U.S.

Validation of Fungicide Spray Strategies and Selection for Fenhexamid Resistance in on Greenhouse-Grown Grapevines.

In this study, in planta assays were conducted to assess the effects of fungicide spray tactics, such as the reduction of the labeled fungicide dose and mixture with a multisite fungicide, on fungicide resistance selection and disease control using 'Cabernet Sauvignon' grown in a greenhouse for 2 years. The entire clusters were inoculated with isolates at varying frequencies of fenhexamid resistance, followed by fungicide sprays and disease and fenhexamid resistance investigations at critical phenological stages. Our findings indicate that the lower dose of the at-risk fungicide, fenhexamid, effectively managed fenhexamid resistance and disease as well as the higher, labeled dose.

Dynamic antimicrobial resistance and phylogenomic structure of Typhimurium from 2007 to 2019 in Shanghai, China.

Unlabelled: serovar Typhimurium is an important foodborne pathogen associated with human salmonellosis worldwide. A retrospective screening was performed to elucidate the prevalence, antimicrobial resistance, and phylogenomic characterization of this pathogen in Shanghai, China. .

Giant cell tumor of soft tissue involving thyroid gland: a case report and review of the literature.

Background: Giant cell tumor of soft tissue is a low malignant uncommon neoplasm, with histologic features and immunophenotype similar to its bone counterpart. Primary giant cell tumor of soft tissue in the thyroid gland is considered an exceedingly rare entity.

Case Presentation: We describe a case of primary thyroid giant cell tumor of soft tissue in a 69-year-old Chinese female patient.

Capture of CO during the Electrolysis Process and Its Utilization in Supercapacitor Materials.

With climate change and environmental issues, the emissions of CO and its greenhouse effect have become a focal point. At present, the utilization of CO includes its synthesis into chemicals and fuels such as methane, methanol, and CO. CO utilization can be achieved through carbon capture and storage technologies, which involve capturing CO from industrial emissions and storing it to reduce the CO concentration in the atmosphere.

Diffuse Associations Between and Filamentous Fungal Microbes May Alter Caneberry Disease Dynamics.

Interactions between microorganisms and frugivorous insects can modulate fruit rot disease epidemiology. Insect feeding and/or oviposition wounds may create opportunities for fungal infection. Passive and active dispersal of fungal inoculums by adult insects also increases disease incidence.

Utilizing Disease Prediction Models to Time Fungicide Applications for Controlling Ripe Rot, Caused by spp., in Maryland Vineyards.

Two previously published ripe rot prediction models, DF2-NN and GH2-DT, were evaluated for fungicide application timing efficacy in Maryland vineyards. Both models utilize leaf wetness duration (LWD), temperature, and grape cluster phenological stages as model parameters. These three parameters were tracked throughout the 2021 to 2023 seasons in three vineyards.

CRISPR-enabled investigation of fitness costs associated with the E198A mutation in β-tubulin of .

Introduction: Understanding fitness costs associated with fungicide resistance is critical to improve resistance management strategies. E198A in b-tubulin confers resistance to the fungicide thiophanate-methyl and has been widely reported in several plant pathogens including .

Method: To better understand potential fitness costs associated with the resistance, a ribonucleoprotein (RNP) complex mediated CRISPR/Cas9 system was used to create a point mutation (E198A) through homology directed repair (HDR) in each of the sensitive (E198) isolates collected from strawberries, raspberries, and peaches.

Preparation of VZrHfNbTa High-Entropy Alloy-Based High-Temperature Oxidation-Resistant Coating and Its Bonding Mechanism.

Ultra-high Temperature Oxidation-Resistant Alloys (UTORAs) have received a lot of attention due to the increased research demand for deep space exploration around the world. However, UTORAs have the disadvantages of easy oxidation and chalking. So, in this study, a UTORAs is prepared by hot-press sintering on VZrHfNbTa (HEA: High Entropy Alloys can generally be defined as more than five elements by the equal atomic ratio or close to the equal atomic ratio alloying, the mixing entropy is higher than the melting entropy of the alloy, generally forming a high entropy solid solution phase of a class of alloys.

Postinfection Application of Fenhexamid at Lower Doses in Conjunction with Captan Slowed Fungicide Resistance Selection in on Detached Grape Berries.

Fungicide resistance is a limiting factor in sustainable crop production. General resistance management strategies such as rotation and mixtures of fungicides with different modes of action have been proven to be effective in many studies, but guidance on fungicide dose or application timing for resistance management remains unclear or debatable. In this study, and the high-risk fungicide fenhexamid were used to determine the effects of fungicide dose, mixing partner, and application timing on resistance selection across varied frequencies of resistance via detached fruit assays.

Acid Radical Tolerance of Silane Coatings on Calcium Silicate Hydrate Surfaces in Aggressive Environments: The Role of Nitrate/Sulfate Ratio.

Silane is known as an effective coating for enhancing the resistance of concrete to harmful acids and radicals that are usually produced by the metabolism of microorganisms. However, the mechanism of silane protection is still unclear due to its nanoscale attributes. Here, the protective behavior of silane on the calcium silicate hydrate (C-S-H) surface is examined under the attack environment of nitrate/sulfate ions using molecular dynamics simulations.

PhoPQ Regulates Quinolone and Cephalosporin Resistance Formation in Salmonella Enteritidis at the Transcriptional Level.

The two-component system (TCS) PhoPQ has been demonstrated to be crucial for the formation of resistance to quinolones and cephalosporins in Enteritidis ( Enteritidis). However, the mechanism underlying PhoPQ-mediated antibiotic resistance formation remains poorly understood. Here, it was shown that PhoP transcriptionally regulated an assortment of genes associated with envelope homeostasis, the osmotic stress response, and the redox balance to confer resistance to quinolones and cephalosporins in Enteritidis.

Identification and Pathogenicity of , , and spp. Associated with Late-Season Bunch Rots of Grape.

Fungal pathogens continue to pose a significant threat to grape production. Previous studies of pathogens associated with late-season bunch rots in Mid-Atlantic vineyards had elucidated the primary causal agents of these diseases, but the significance and identity of the less commonly isolated genera was unclear. Therefore, to more fully understand the identity and pathogenicity of , , and spp.

Effects of Variation in Plantations on Soil Microbial Community Composition in the Middle Yellow River Floodplain.

Floodplains have important ecological and hydrological functions in terrestrial ecosystems, experience severe soil erosion, and are vulnerable to losing soil fertility. Lour. plantation is the main vegetation restoration measure for maintaining soil quality in floodplains.

Crystal Structure Analysis of Sarcoplasmic-Calcium-Binding Protein: An Allergen in .

The structure of allergenic proteins provides important information about the binding of allergens to antibodies. In this study, the crystal structure of Scy p 4 with a resolution of 1.60 Å was obtained by X-ray diffraction.

Off-target selection of resistance to azoxystrobin in Aspergillus species associated with grape late season rots.

Due to recent evidence of Aspergillus uvarum pathogenicity on wine grapes and variable fungicide sensitivity to quinone outside inhibitor (QoI) fungicides, the identity and QoI sensitivity of Aspergillus isolates from the Mid-Atlantic United States was investigated. Phylogenic analysis of 31 isolates revealed 26 as A. uvarum and 5 as A.

Correlation between PD-L1 expression of the tumour cells and lymphocytes infiltration in the invasive front of urothelial carcinoma.

Purpose: Programmed cell death-ligand 1 (PD-L1) as a cell surface glycoprotein can inhibit T cell function when binding to its receptor, PD-1. The newly developed therapy of targeting PD-1/PD-L1 signal pathway has shown great promise for the treatment of non-small cell lung cancer as well as melanoma. Approved by Food and Drug Administration, atezolizumab has become the first new drug to treat advanced bladder cancer.

Characterization of the Role of Two-Component Systems in Antibiotic Resistance Formation in Salmonella enterica Serovar Enteritidis.

The two-component system (TCS) is one of the primary pathways by which bacteria adapt to environmental stresses such as antibiotics. This study aimed to systematically explore the role of TCSs in the development of multidrug resistance (MDR) in Salmonella enterica serovar Enteritidis. Twenty-six in-frame deletion mutants of TCSs were generated from .

Nomogram of intra-abdominal infection after surgery in patients with gastric cancer: A retrospective study.

Background: Surgical resection is still the primary way to treat gastric cancer. Therefore, postoperative complications such as IAI (intra-abdominal infection) are major problems that front-line clinical workers should pay special attention to. This article was to build and validate IAI's RF (regression function) model.

Phylogenomic Analysis of Salmonella enterica Serovar Indiana ST17, an Emerging Multidrug-Resistant Clone in China.

Salmonella enterica serovar Indiana ( Indiana) is an extremely expanded foodborne pathogen in China in recent years. This study aimed to elucidate the national prevalence and phylogenomic characterization of this pathogen in China. Among 5, 287 serotyped Salmonella isolates collected during 2002 to 2018, 466 Indiana isolates were found in 15 provinces, and 407 were identified to be ST17, and the rest were ST2040.

[2 + 2] Cycloaddition of Ynamides to Construct 3-Aminocyclobutenones.

An efficient TfNH-catalyzed [2 + 2] cycloaddition of ynamides under mild conditions has been developed. Within short reaction times, various ynamides are transformed into the corresponding 3-aminocyclobutenones in good to excellent yields. This is the first example for the metal-free intermolecular [2 + 2] self-cycloaddition of ynamides.