Synthesis of a novel adamantyl nitroxide derivative with potent anti-hepatoma activity in vitro and in vivo.

In this study, a novel adamantyl nitroxide derivative was synthesized and its antitumor activities in vitro and in vivo were investigated. The adamantyl nitroxide derivative 4 displayed a potent anticancer activity against all the tested human hepatoma cells, especially with IC50 of 68.1 μM in Bel-7404 cells, compared to the positive control 5-FU (IC50=607.

[Effects of nuclear factor of activated T cells on the promoter activity of constitutively activated SV40].

Aim: To check if the common used constitutively promoters, such as CMV, TK and SV40 could be responded to the nuclear factor of activated T cell (NFAT), and to explore the strategies to choose rational internal control in the dual luciferase reporter assay.

Methods: pCMV-luc vector, in which luciferase activity is driven by CMV promoter, was cloned by amplifying the CMV promoter fragment from the pCDNA3.1 vector and then inserting the CMV promoter region into the pGL3-basic vector using the standard protocol.