Structural characterization and immunosuppressive activity of a new pregnane glycoside from .

Phytochemical investigation on the ethyl acetate fraction of the leaves of led to the isolation of a new C pregnane glycoside, epigycoside B (), together with three known analogues. Their structures were elucidated on the basis of extensive spectroscopic techniques, including UV, MS, and NMR experiments, as well as the chemical methods. Compound displayed immunosuppressive activity against concanavalin A (Con A)/Lipopolysaccharides (LPS)-stimulated proliferation of mice splenocyte.

tert-Butyl 6-amino-3,4-dihydro-2H-1,4-benzoxazine-4-carboxyl-ate.

The title mol-ecule, C(13)H(18)N(2)O(3), contains a benzene ring fused to an oxazine ring and one tert-but-oxy-carbonyl group bound to the N atom of the oxazine ring. A weak intra-molecular C-H⋯O inter-action occurs. In the crystal, inter-molecular N-H⋯O and C-H⋯O hydrogen bonds stack the mol-ecules down the b axis.

3-(2-Fluoro-phen-oxy)propanoic acid.

In the title compound, C(9)H(9)FO(3), the dihedral angle between the carboxyl group and the benzene ring is 79.4 (3)°. In the crystal, mol-ecules form centrosymmetric dimers through pairs of classical O-H⋯O hydrogen bonds.

[In vitro inhibitory effect of 23-HBA on angiogenesis].

Aim: To study the inhibitory effect of 23-HBA on angiogenesis in vitro.

Methods: The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed.