Publications by authors named "Meng-die Ding"
Article Synopsis
- Researchers studied lactic acid bacteria (LAB) in Tibetan chickens to find safe strains that could deliver foreign proteins for vaccines.
- They identified a strain called Lactobacillus salivarius TCMM17, which strongly adheres to chicken gut cells and shows no harmful effects or resistance to antibiotics.
- By inserting a specific gene into TCMM17, they created a modified strain that can maintain this gene over generations and display the related protein on its surface, paving the way for new oral vaccines against bird diseases.
Objective: To assemble infectious bronchitis virus (IBV)-like particles bearing the recombinant spike protein and investigate the humoral immune responses in chickens.
Results: IBV virus-like particles (VLPs) were generated through the co-infection with three recombinant baculoviruses separately encoding M, E or the recombinant S genes. The recombinant S protein was sufficiently flexible to retain the ability to self-assemble into VLPs.
Objectives: To develop a cost-effective ELISA for detection of antibodies against infectious bronchitis virus (IBV) by using a multi-fragment protein as coating antigen.
Results: A multi-fragment antigen, termed BE, which was composed of eight antigenic fragments selected from the three major proteins (S, M, and N) of IBV, was expressed in Escherichia coli. The entire protein had a molecular weight of 61.
An indirect enzyme-linked immunosorbent assay (ELISA) method based on a novel multi-epitope antigen of S protein (SE) was developed for antibodies detection against infectious bronchitis virus (IBV). The multi-epitope antigen SE protein was designed by arranging three S gene fragments (166-247 aa, S1 gene; 501-515 aa, S1 gene; 8-30 aa, S2 gene) in tandem. It was identified to be approximately 32 kDa as a His-tagged fusion protein and can bind IBV positive serum by western blot analysis.
View Article and Find Full Text PDFMucosal immunity is critical in preventing infectious bronchitis virus (IBV) infection. To deliver viral antigens to the mucosal immune system of chickens safely and effectively, we constructed a Lactococcus lactis strain carrying IBV multi-epitope gene EpiC fused with the gene of the cell-wall anchoring domain of Staphylococcus aureus protein A. SDS-PAGE and Western blot results indicated that the fused peptide was located partially on the cell surface.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the avian infectious bronchitis virus (IBV) and the effectiveness of a multi-epitope peptide called EpiC in generating immune responses.
- Researchers introduced the EpiC gene into Lactococcus lactis NZ3900 to create recombinant strains that can express the peptide in different forms.
- Oral vaccination of chickens with these recombinant strains led to stronger immune responses and protection against a lethal IBV strain, suggesting L. lactis could be a viable option for oral vaccines against IBV infection.