Amoxicillin degradation and high-value extracellular polymer recovery by algal-bacterial symbiosis systems.

Algal-bacterial symbiosis systems have emerged as sustainable methods for the treatment of new pollutants and the recovery of resources. However, the bio-refinery of biomass derived from microalgae is inefficient and expensive. In order to simultaneously degrade antibiotic and recover resources efficiently, two algal-bacterial symbiosis systems were constructed using Pseudomonas aeruginosa (alginate overproduction) and Bacillus subtilis (poly-γ-glutamic acid overproduction) with amoxicillin-degrading-microalga Prototheca zopfii W1.

Insight into the structure and metabolic function of iron-rich nanoparticles in anammox bacteria.

Anaerobic ammonium-oxidizing (anammox) bacteria are iron abundant and depend heavily on iron-binding proteins. The iron demand of anammox bacteria is relatively large. However, it still remains some doubts where these large quantities of available iron come from and how they are regulated in anammox bacteria.

Potential role of nanobubbles in dynamically modulating the structure and stability of anammox granular sludge within biological nitrogen removal process.

The generation of visible macrobubbles considerably affects the structure and function of anammox granules in the anammox granular sludge (AnGS) system. However, the existence of nanobubbles (NBs) and their role in maintaining the AnGS structure and stability are unclear because of the complexity of the system and lack of effective analytical methods. In this study, methods for NB analysis and assessment of their effects were developed to investigate the formation and characteristics of NBs in an AnGS system and the effects of NBs on the properties and function of AnGS.

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria.

Anaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to its function; however, research on the ultrastructure of intact anammox bacteria is lacking.

Underlying Promotion Mechanism of High Concentration of Silver Nanoparticles on Anammox Process.

Silver nanoparticles (AgNPs) are largely discharged into sewers and mostly accumulated in the sediments and sludge. The toxicity of AgNPs to environmental microorganisms has attracted great attention. However, the effect of AgNPs on anaerobic ammonium-oxidizing (anammox) granules remains unknown.

Identification of ceftazidime interaction with bacteria in wastewater treatment by Raman spectroscopic mapping.

Raman spectroscopy yields a fingerprint spectrum and is of great importance in medical and biological sciences as it is non-destructive, non-invasive, and available in the aqueous environment. In this study, Raman spectroscopy and Raman mapping were used to explore the dynamic biochemical processes in screened bacteria under ceftazidime stress. The Raman spectral difference between bacteria with and without antibiotic stress was analyzed by principal component analysis and characteristic peaks were obtained.

Extracellular polymeric substances dependence of surface interactions of Bacillus subtilis with Cd and Pb: An investigation combined with surface plasmon resonance and infrared spectra.

Microbial extracellular polymeric substances (EPS) play an important role in resisting the shock load of toxic contaminants to microbial aggregates. In order to investigate the surface interaction process of bacteria with heavy metals, in this work, the kinetics and affinity of heavy metal (CdCl and PbCl) binding on Bacillus subtilis with EPS and without EPS were determined using in situ surface plasmon resonance. The binding mechanism between bacteria (with EPS and without EPS) and heavy metals was probed using Fourier-transform infrared spectra.