The potential application of a poly(3,4-ethylenedioxythiopene) modified platinum DNA biosensor in mutation analysis.

In this study, we have fabricated a label free DNA biosensor by modifying the platinum wire with electrochemically synthesized poly(3,4-ethylenedioxythiopene) and poly(p-aminobenzoic acid). A designed single-strand DNA oligo was immobilized with the carboxyl group of poly(p-aminobenzoic acid) and served as the probe, a target DNA was then hybridized with the probe under a proper condition. Differential pulse voltammetry was performed to characterize the hybridization efficiency in the presence of daunorubicin hydrochloride that was able to be intercalated into the hybridized double-strand DNA and possessed the redox activity.