The Influences of Sports Psychological Capital to University Baseball Athletes' Life Stress and Athlete Burnout.

Previous studies suggested that athletes' psychological capital level is related to life stress and burnout. Therefore, the purpose of this study was to explore the influences of university baseball athletes' psychological capital on their life stress and burnout and provide practical suggestions for athletes and coaches to reduce their life stress and burnout. In this study, we used athletes' control variables (grade, year of training experience, and training days per week) and psychological capital (self-efficacy, hope, optimism, and resilience) to predict their life stress and burnout.

The Relationships among Sport Participation Level, Flow Experience, Perceived Health Status and Depression Level of College Students.

The purpose of this study was to examine the relationships between sport participation level, flow, perceived health status and depression using gender and grades as control variables of college students in Taiwan. Based on previous research, the study established the proposed model: using sport participation level and flow experience as predicting variables, perceived health status and depression as dependent variables, and gender and grades as control variables. A total of 700 structured questionnaires were distributed to college students using convenience sampling among seven universities in Taiwan with a valid return rate of 86.

The efficacy of ethnic specific blood groups genotyping for routine donor investigation and rare donor identification in Taiwan.

Background: Large-scale single nucleotide variation (SNV)-based blood group genotyping assays have been made available for over a decade. Due to differences in ethnic groups, there is much diversity in clinically important blood group antigens and genetic variants. Here, we developed a robust matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-based blood group genotyping method on MassARRAY system.

Universal Detection of Mi Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi (MNS7), Mur (MNS10), and MUT (MNS35) Antigens.

Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.

A large-scale phylogeny of Microhylidae inferred from a combined dataset of 121 genes and 427 taxa.

The phylogenetic relationships of Microhylidae, the third largest family of extant frogs, have been difficult to resolve. In the past decade, large amounts of sequence data have been deposited for almost every microhylid genus, but no study has attempted to combine these data to reconstruct a comprehensive phylogeny for this family. In this study, we sequenced 20 near-complete or partial microhylid mitochondrial genomes and integrated them with all available sequences of Microhylidae from GenBank to construct a supermatrix containing 121 genes (14 mitochondrial and 107 nuclear protein-coding genes).

Sinorhizobium meliloti ExoR is the target of periplasmic proteolysis.

Sinorhizobium meliloti ExoR regulates the production of succinoglycan and flagella through the ExoS/ChvI two-component regulatory system. ExoR has been proposed to inhibit the ExoS sensor through direct interaction in the periplasm. To understand how ExoR suppression of ExoS is relieved, which is required for the expression of ExoS/ChvI-regulated symbiosis genes, we characterized wild-type ExoR and ExoR95 mutant proteins.

Inhibition of tankyrase 1 in human gastric cancer cells enhances telomere shortening by telomerase inhibitors.

Telomere stability is believed to be related to aging and tumorigenesis. Besides telomerase, telomere length is also regulated by several telomere-specific binding proteins. Tankyrase 1, a telomeric poly(ADP-ribose) polymerase (PARP), elongates telomere length by inhibiting TRF1 binding to telomeres.

The efficacy of individual-donation and minipool testing to detect low-level hepatitis B virus DNA in Taiwan.

Background: Financial constraints are the main concern in implementing nucleic acid testing (NAT) as routine blood screening in Taiwan. The PROCLEIX ULTRIO assay (Ultrio) on the TIGRIS System (Novartis Diagnostics) was evaluated for its operational performance both for individual-donation testing (IDT) and in minipools of 4 (MP4) to develop a feasible solution.

Study Design And Methods: Analytical sensitivity was determined by testing WHO international standards.

[Screening for autoinducer synthase gene in Sinorhizobium meliloti and analysis of the autoinducer produced by recombinant expression in Escherichia coli].

The plasmid pJZ290 containing mariner transposon was used to mutagenize Sinorhizobium meliloti and 3000 transposon insertion mutants were subsequently screened for autoinducer-deficient (AI-deficient) mutants. One AI-deficient mutant YW1 was obtained by quantitative activity assay and qualitative TLC detection. In an effort to characterize the transposon insertions of autoinducer-deficient mutant YW1, we performed an arbitrary PCR and sequencing techniques to identify insertion sites.

Three missense mutations, including a novel 860C>T transition, and allelic enhancement phenomenon associated with ABO blood subgroups A in Taiwan.

Background: It was estimated that approximately 25 percent of Taiwanese residents were ABO blood group A. Many subgroups of A, however, revealed ambiguous serologic typing results. This study aimed to delineate the molecular basis of the A3, Ax, and weak A phenotypes.

[Recombinant expression of an autoinducer synthase of Mesorhizobium loti in Escherichia coli and analysis of the autoinducer produced in recombinant strain].

Although the autoinducers of Mesorhizobium loti could not be detected under the conditions of experimentally pure culture, and even adding some root exact of Lotus japonicus into the medium in which M. loti grew to detect the acyl-homoserine lactone (AHL) production, no positive result has been available. However, analysis of homologous comparison showed that there were at least four annotated acylated homoserine lactone (AHL) synthase genes from M.

[High-level expression of an extreme-thermostable xylanase B from Thermotoga maritima MSB8 in Escherichia coli and Pichia pastoris].

The family 10 xylanase (XynB) from Thermotoga maritima MSB8 is extremely thermostable and great potential in the applications of various fields of industry. The gene mxynB(64) was amplified by the method of PCR with the template of the genomic DNA of Thermotoga maritima MSB8, and cloned into the expression vectors of Escherichia coli and Pichia pastoris respectively. Xylanase B(40kD) was successfully expressed by the two heterologous protein expression systems with high-level production.

[Increased expression of heparanase mRNA is correlated with metastasis of gastric carcinoma and is probably correlated with c-met protein].

Objective: To explore the expression of human heparanase mRNA and its relationship with the clinicopathological characteristics and angiogenesis and its possible mechanism in gastric carcinomas.

Methods: Expression of heparanase mRNA in gastric cancerous tissues and its corresponding adjacent tissues were detected by RT-PCR in forty-seven patients. Expression of c-met protein and microvessel density (MVD) were examined by immunohistochemcal staining.