[Cochlear implantation and rehabilitation in prelingually deafened adolescents and young adults].

Objective: To investigate the outcomes of prelingually deafened adolescents and young adults receiving cochlear implantation (CI).

Methods: Thirty-three patients with prelingual deafness aged 7-33 years at the time of CI were followed up for 4 years. The Speech Discrimination Score (SDS) was tested using disyllabic words and short sentences with close-set and open-set, respectively.

[Therapeutic effect of ossicular reconstruction with bioceramic or porous macromolecular polyethylene partial ossicular replacement prosthesis in patients with tympanosclerosis].

Objective: To evaluate the effect of ossicular reconstruction with partial ossicular replacement prosthesis (PORP) in patients with tympanosclerosis.

Methods: The data of 31 cases of tympanosclerosis treated between 1992 and 2009 were reviewed. Of the 31 patients, 17 (17 ears) underwent ossicular reconstruction with porous macromolecular polyethylene PORP, and 14 (14 ears) with bioceramic PORP.

[Study of three-dimensional speech chart by time-frequency analysis in Chinese Mandarin Monosyllabic word phonemes].

Objective: To investigate the physical characteristic of Chinese Mandarin Monosyllabic word phonemes by the time-frequency analysis.

Methods: Manufactured and study the three-dimensional speech chart which was on the basic of information of the time-frequency analysis in 40 monosyllabic words with the SigmaPlot 9.0 and Matlab 7.

[Tumor-targeted human telomerase reverse transcriptase promoter/tk gene therapy against human nasopharyngeal carcinoma cells in vitro].

Objective: To investigate the targeted killing effect of human telomerase reverse transcriptase promoter (hTERTp)/tk gene on human nasopharyngeal carcinoma (NPC) cells.

Methods: The recombinant plasmid hTERTp/tk/pGL3 was transfected into human NPC HNE1 cells and the expressions of TK and telomerase were investigated. The targeted killing effect induced by hTERTp/tk on HNE1 cells was assessed using RT-PCR and MTT assay.

[Clinical analysis of 118 patients with tympanosclerosis].

Objective: To study the incidence of tympanosclerosis (TS) and the major risk factors of hearing loss.

Methods: A total of 118 TS patients (137 ears) were compared with 265 patients with chronic otitis media (COM) (311 ears) for gender, age and course of disease. The disease regions and hearing loss of the TS patients were analyzed.

[Cochlear implant in patients with congenital malformation of the inner ear].

Objective: To summarize the clinical experience with multi-channel cochlear implantation in patients with inner ear malformations and evaluate and the outcomes of speech rehabilitation.

Methods: A retrospective study was conducted in 295 patients receiving cochlear implantation from 1998 to 2007, including 25 patients with large vestibular aqueduct syndrome (LVAS), 9 with Modini malformation, and 5 with common cavity deformity. All the patients received the Nucleus24 cochlear implants.

[Cochlear implantation in patients with large vestibular aqueduct syndrome].

Objective: To evaluate the hearing and speech recognition in patients with large vestibular aqueduct syndrome undergoing multi-channel cochlear implantation.

Methods: From 2005 to 2008, multi-channel cochlear implantation surgeries were performed in 22 patients with large vestibular aqueduct syndrome. All the patients received multi-channel cochlear implantation through transmastoid facial recess approach.

[Effect of surgical reconstruction of congenital aural atresia via the mastoid antrum approach: analysis of 48 cases].

Objective: To assess the effect of surgical reconstruction of congenital aural atresia via the mastoid antrum approach and investigate method for preventing postoperative atresia of the reconstructed aural canal.

Methods: From 2000 to 2008, aural canal reconstruction and tympanoplasty was performed via the mastoid antrum approach. In 48 patients with congenital aural atresia (54 ears, including 45 ears of type II, 9 ears of type III).

[Therapeutic effects of small fenestra stapedotomy with semiconductor diode laser: a comparison with microdrill].

Objective: To compare the effects of small fenestra stapedotomy with semiconductor diode laser and microdrill in patients with otosclerosis.

Methods: Twenty-six patients (29 ears) undergoing stapedotomy with semiconductor diode laser and 19 patients (21 ears) with microdrill were compared for the hearing results and complication rates.

Results: No statistically significant differences were found in postoperative speech frequency and high frequency pure tone average in closing the air-bone gap between the two groups.

[Therapeutic effect of insulin-like growth factor-1 injection into the inner ears through scala tympani fenestration on gentamicin-induced hearing loss in guinea pigs].

Objective: To study the therapeutic effect of insulin-like growth factor-1 (IGF-1) injection into the inner ears through a scala tympani fenestration on sensorineural deafness in a guinea pig model of gentamicin-induced hearing loss.

Methods: Twenty guinea pigs with gentamicin-induced hearing loss were randomized equally into IGF-1 group and control group. In both groups, scala tympani fenestration was performed for injection of IGF-1 (10 microl) or artificial perilymphatic fluid (10 microl).

[Auditory brainstem response in normal guinea pigs and those with gentamicin-induced hearing loss under awake and anesthetic conditions].

Objective: To observe the alterations of auditory brainstem response (ABR) in guinea pigs with gentamicin-induced hearing loss under awake and anesthetic conditions.

Methods: We recorded the ABR in 20 normal guinea pigs and 20 with gentamicin-induced hearing loss before and after anesthesia for statistical analysis.

Results: No significant difference was observed in the waveform, response threshold (RT), I and III peak latencies (PL), I-III interpeak latencies (IPL) of ABR between awake and anesthetic conditions in normal guinea pigs (P>0.

[Role of ciliary neurotrophic factor in regeneration of severed facial nerve of cats].

Objective: To study the role of extrinsic (CNTF) in the regeneration of severed facial nerve in cats.

Method: The facial nerve in temporal bone of adult cats were severed and the severed ends were connected with CNTF or saline applied at the connection. Electrophysiological examination and immunocytochemistry were performed with immunoelectron microscope for morphological analysis at 2, 4 and 8 weeks after the operation.

[Subcloning of human neurotrophin-3 gene and construction of its genetically engineered cell model].

Objective: To subclone human neurotrophin-3 gene (NT3) and transfer this gene into human bone marrow mesenchymal stem cells (BM-MSCs) to construct genetically engineered cells that produce NT3 in vitro.

Methods: Human BM-MSCs were cultured in low-glucose DMEM supplemented with 10% fetal bovine serum and 10 ng/ml epidermal growth factor. Flow cytometry (FCM) was used to examine the phenotypes of the cells.

[Two cases of ectopic thyroid tumor].

We report our experience with 2 cases of ectopic thyroid tumor and on the basis of literature review, attempting to investigate the origin, clinical manifestations, diagnosis and treatment of this disease.

[Telomere shortening in the pathogenesis of nasopharyngeal carcinoma].

Objective: To explore the role of telomere shortening in the pathogenesis of nasopharyngeal carcinoma (NPC).

Methods: Southern blotting was employed to determine the mean telomere length (MTL) in 42 tumor samples and 15 samples of the tissues adjacent to the tumors obtained from 42 NPC patients, along with the tissue specimens from 17 patients with chronic nasopharyngitis.

Results: In NPC tissues, the length of the MTL was 4.

Resection of nasopharyngeal angiofibroma with extranasal and extrapharyngeal involvement.

The authors report their own experiences with the diagnosis and therapy of 7 cases of nasopharyngeal angiofibroma with extranasal and extrapharyngeal involvement, thereby attempt to formulate the surgical approach for resection of huge nasopharyngeal angiofibroma with extensive involvement. The results suggested that appropriate surgical approach was of paramount importance for eliminating the tumor and may effectively reduce traumatic injury during the operation. Elective embolization of the vessels feeding the tumor could reduce bleeding and therefore increase the safety of the operation.