[Effects of elevated atmospheric CO concentration on chemical composition of rice straw].

Rice straw is a potential material for bioenergy production. Elevated atmospheric carbon dioxide (CO) concentration changed the quantity and quality of rice straw, thus changing its bioenergy production potential. In this experiment, we collected rice straw from China Free Air CO Enrichment Platform (FACE).

[Transformation of full-length gene of mouse/human chimeric antibody against Hantaan virus into Arabidopsis thaliana].

Aim: To construct the transgenic Arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3G1MH) against Hantaan virus.

Methods: The recombinant plasmid 3G1MH-pCAMBIA2301 was transformed into Agrobacterium tumefaciens GV3101 by TSS freeze-thaw method, and then the recombinant was transferred into wild Arabidopsis thaliana by vacuum-transgenic method. The regenerated transgenic plants were selected with kanamycin, and confirmed by PCR and Northern blot.

[Expression and genetic immunization of hantaan virus G2 recombinant adenovirus].

Aim: To express hantaan virus(HTNV) envelope glycoprotein G(2) recombinant adenovirus(Adeno-G(2)) in vero E6 cells and explore its property of inducing immune response.

Methods: Vero E6 cells were infected with the HTNV Adeno-G(2) (100 MOI). The expression of Adeno-G(2) in the infected Vero E6 cells was detected by IFA.