Aqueous Solution Enhanced Room Temperature Phosphorescence through Coordination-Induced Structural Rigidity.

Achieving aqueous solution enhanced room temperature phosphorescence (RTP) is critical for the applications of RTP materials in solution phase, but which faces a great challenge. Herein, for the first time, a strategy of coordination-induced structural rigidity is proposed to achieve enhanced quantum efficiency of aluminum/scandium-doped phosphorescent microcubes (Al/Sc-PMCs) in aqueous solution. The Al/Sc-PMCs in a dry state exhibit a nearly invisible blue RTP.

Inorganic salt recrystallization strategy for achieving ultralong room temperature phosphorescence through structural confinement and aluminized reconstruction.

Achieving highly efficient and stable room temperature phosphorescence (RTP) with ultralong lifetime is critical for the multi-purpose applications of phosphorescent materials. In this work, we propose an inorganic salt heating recrystallization strategy to simultaneously improve the lifetime, quantum efficiency, and stability of phosphorescent scandium/leucine microspheres (Sc/Leu-MSs). Inorganic salt-treated Sc/Leu-MSs are obtained by simply heating and drying inorganic salt solution containing Sc/Leu-MSs, which can achieve a maximum lifetime increase of 4.

A general method for rapid synthesis of refractory carbides by low-pressure carbothermal shock reduction.

Refractory carbides are attractive candidates for support materials in heterogeneous catalysis because of their high thermal, chemical, and mechanical stability. However, the industrial applications of refractory carbides, especially silicon carbide (SiC), are greatly hampered by their low surface area and harsh synthetic conditions, typically have a very limited surface area (<200 m g), and are prepared in a high-temperature environment (>1,400 °C) that lasts for several or even tens of hours. Based on Le Chatelier's principle, we theoretically proposed and experimentally verified that a low-pressure carbothermal reduction (CR) strategy was capable of synthesizing high-surface area SiC (569.

In Situ Imaging of Ion Motion in a Single Nanoparticle: Structural Transformations in Selenium Nanoparticles.

Intraparticle ion motions are critical to the structure and properties of nanomaterials, but rarely disclosed. Herein, in situ visualization of ion motions in a single nanoparticle is presented by dark-field microscopy imaging, which shows HgCl -induced structural transformation of amorphous selenium nanoparticles (SeNPs) with the main composition of Se . Owing to the high binding affinity with selenium and coulomb interactions, Hg ions can permeate into the interior of SeNPs, making the amorphous Se turn to polycrystalline Hg Se Cl .

[Cloning and prokaryotic expression of 3-ketoacyl-CoA thiolase gene AIKAT from Atractylodes lancea].

In this study, the gene encoding the key enzyme 3-ketoacyl-CoA thiolase(KAT) in the fatty acid β-oxidation pathway of Atractylodes lancea was cloned. Meanwhile, bioinformatics analysis, prokaryotic expression and gene expression analysis were carried out, which laid a foundation for the study of fatty acid β-oxidation mechanism of A. lancea.

[Ecological remediation of earthworms on soil-plant system: A review].

Soil-plant system is the basic structural unit of the biosphere, with close mutual feedback between soil and plants. The degradation of soil exerts various abiotic stresses to plants, disturbs the physiological metabolism of plants, and inhibits nutrient acquisition. Earthworms are known as "ecosystem engineers", which can regulate soil physical-chemical-biological characteristics, improve the quality of the degraded soil (saline soil, heavy metals and organic pollutants contaminated soil), alleviate plants under stress, increase soil nutrient availability, promote plant growth.

The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells.

Aims: The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats.

Methods: The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.

Fluorescent carbon dots functionalization.

Carbon dots (CDs), as a new type of luminescent zero-dimensional carbon nanomaterial, have been applied in a variety of fields. Currently, functionalization of CDs is an extremely useful method for effectively tuning their intrinsic structure and surface state. Heteroatom doping and surface modification are two functionalization strategies for improving the photophysical performance and broadening the range of applications for fluorescent CDs.

Metal-Mediated Gold Nanospheres Assembled for Dark-Field Microscopy Imaging Scatterometry.

Developing rapid, sensitive and intelligent optical probes is important for the growing need of microscope imaging analysis. Herein, we proposed a new strategy to assemble plasmonic nanoprobes in situ for dark-field microscopy imaging scatterometry by making use of the formation, disruption, and re-formation of cytosine-Ag-cytosine (CAgC) bonds. The CAgC bond was formed at first through Ag-mediated base pairing between C-contained aptamer and its C-mismatched complementary DNA.

A single gold nanoprobe for colorimetric detection of silver(i) ions with dark-field microscopy.

Highly sensitive colorimetric detection of silver(i) ions (Ag+) at the single-particle level was developed based on the color of a gold nanoparticle (AuNP) assembly captured by dark-field microscopy (DFM) imaging. Formation of C-Ag+-C bonding between cytosines was utilized to induce interparticle coupling of AuNPs modified with single-strand DNA, resulting in a color change as the signal transduction to quantify Ag+ under DFM imaging. This method allowed visual quantitation of Ag+ in the range of 0.

Anthrax biomarker: An ultrasensitive fluorescent ratiometry of dipicolinic acid by using terbium(III)-modified carbon dots.

The highly sensitive detection of dipicolinic acid (DPA), a biomarker of the biological threat-agent anthrax, is strongly associated with the sensing of Bacillus anthracis (B. anthracis) in environmental and food samples. In this study, we developed a novel, ultrasensitive method for the detection of DPA by using a ratiometric fluorescent terbium ions modified carbon dots (CDs-Tb).

Terbium(III) Modified Fluorescent Carbon Dots for Highly Selective and Sensitive Ratiometry of Stringent.

Highly selective and sensitive detection of guanosine 3'-diphosphate-5'-diphosphate (ppGpp), namely, the stringent in plants or microorganisms responding to strict or extreme environmental conditions such as stress and starvation, which plays an important role in gene expression, rRNA and antibiotics production, regulations of virulence of bacteria, and growth of plants, faces a great challenge owing to its extreme similarity to normal nucleotides. By modifying the surface groups of a facile two-step hydrothermal route prepared carbon dots (CDs) with terbium ions (Tb) in this contribution, a novel fluorescent probe with excellent properties such as highly physical and chemical stability, narrow emission and excitation wavelength-independent emission was prepared. The Tb ions on the surface of CDs cannot only preserve the intrinsic fluorescence (FL) of CDs but also keep its own coordination capacity with rare earth complex, and thus the clamp structure (four phosphate groups) of ppGpp can specific binding with Tb ions on the surface of CDs to produce antenna effect.

HLA-B*07, HLA-DRB1*07, HLA-DRB1*12, and HLA-C*03:02 Strongly Associate With BMI: Data From 1.3 Million Healthy Chinese Adults.

Strong associations between HLA alleles and infectious and autoimmune diseases are well established. Although obesity is also associated with these diseases, the relationship between HLA and obesity has not been systematically investigated in a large cohort. In the current study, we analyzed the association of HLA alleles with BMI using data from 1.

Boron and nitrogen co-doped single-layered graphene quantum dots: a high-affinity platform for visualizing the dynamic invasion of HIV DNA into living cells through fluorescence resonance energy transfer.

High-affinity binding of carbon nanomaterials with nucleobases, which is still a challenge, is the basis for DNA directed assembly and sensing. In this work, boron and nitrogen co-doped single-layered graphene quantum dots (BN-SGQDs) are designed as a high-affinity platform for nucleic acid detection and imaging in living cells, which has been confirmed by density functional theory (DFT) simulation and experiments. Owing to their excellent absorption and photoluminescence ability, the high quantum yield (QY 36.

Aptamer-modified selenium nanoparticles for dark-field microscopy imaging of nucleolin.

Selenium nanoparticles (SeNPs) with strong green scattering light serving as light scattering nanoprobes for dark-field microscopy imaging are developed for the first time. The SeNPs show good stability, excellent biocompatibility, and high light-producing power, and can precisely and specifically target cancer cells with overexpressed nucleolin (NCL) after modification with aptamers, revealing that the SeNPs can act as a good light scattering nanoprobe for bioimaging and biolabeling.

Highly selective detection of phosphate ion based on a single-layered graphene quantum dots-Al strategy.

Determination of phosphate ion (PO) is important in biomedical and environmental arrays because its controlling concentrations are associated with different pathologies or the quality of water. Herein, we report a new type of photoluminescence (PL) probe for highly selective detection of PO based on a single-layered graphene quantum dots chelating with aluminium ions (s-GQDs-Al) system. The PL of s-GQDs can be enhanced by Al through the aggregation-induced emission enhancement (AIEE) effect.

Self-exothermic reaction prompted synthesis of single-layered graphene quantum dots at room temperature.

The easy fabrication of single-layered graphene quantum dots (s-GQDs) still faces challenge. Herein, we report an efficient route to fabricate s-GQDs within 5 min at room temperature by introducing a simple self-exothermic reaction. The as-prepared s-GQDs can specifically bind with aluminium ions to produce an aggregation-induced emission enhancement effect.

One-pot carbonization synthesis of europium-doped carbon quantum dots for highly selective detection of tetracycline.

The detection of tetracycline is of great significance because of its damaging effects on human health, such as renal toxicity and hemolytic anemia. Any release of tetracycline into the surrounding environment can produce bacterial drug resistance. We develop a new sensitive and selective detection approach for tetracycline in complex water samples by preparing europium-doped carbon quantum dots (Eu-CQDs) through a simple and rapid carbonization method operating at 200 °C for 5 min.

Highly selective and sensitive detection of 2,4,6-trinitrophenol by using newly developed blue-green photoluminescent carbon nanodots.

Though many methods of detecting 2,4,6-trinitrophenol (TNP) mainly have been developed recent years, quantification of TNP in environmental matrixes still faces up to great challenges because all the nitroaromatic explosives reveal highly similar chemical structure. In the present work, we have developed a selective and sensitive method for detection of TNP by amorphous photoluminescent carbon nanodots (CNDs), which are prepared through a simple hydrothermal route using spermine and m-phenylenediamine (MPD) as precursors. The as-prepared CNDs are found to show blue-green photoluminescence, excitation-wavelength independence, and excellent chemical and optical stability.

[Identification and analysis of the rare HLA-A/B/DRB1 allele genes of 10165 bone marrow registry donors in Shaanxi region].

This study was purposed to analyze the detected status of rare alleles from HLA-A/B/DRB1 typing of 10165 unrelated hematopoietic stem cell donors in Shaanxi region during 2009-2012. The rare allele distributions of HLA-A/B/DRB1 gene typing of 10165 unrelated-donors from Shaanxi sub-registry of Chinese National Marrow Donor Project (CMDP) were detected and analyzed by PCR-SBT. The results showed that there were 40 rare alleles from 48 donors identified by PCR-SBT in 10165 unrelated-donors of Shaanxi sub-registry.

[Analysis of gene polymorphism of HPA and HLA-I in Chinese Xi'an voluntary platelet donors].

To study the allele frequencies and their polymorphism characteristics of human platelet antigen (HPA) and human leucocyte antigen-I (HLA-I) in Chinese xi'an population, the types of HPA and HLA-I in 375 Chinese xi'an voluntary platelet donors were detected by PCR-SSP and PCR-SSO as well as flow cytometry with magnetic beads, and were analyzed. The results showed that there was no polymorphism in HPA-7-HPA-14, HPA-16 and HPA-17 which only expressed-aa type, the -bb type was only detected in HPA-3 and HPA-15, 9 out of 16 samples for the HPA-5ab phenotype simultaneously expressed HPA-15ab, the other 7 samples expressed HPA-15bb, no HPA-15aa phenotype was observed. Phenotypes detected in this study were HPA-1aa-17aa, HPA-1ab, -2ab, -3ab, -3bb, -4ab, -5ab, -6ab, -15ab and -15bb.

[Molecular study of two novel RHD alleles and pedigree analysis].

Objective: To study the segregation of two novel RHD alleles in Chinese pedigrees.

Methods: The Rh antigens of the samples were identified by using monoclonal antibodies. The 10 exons of the RHD gene for the 2 probands and their family members were amplified separately and sequenced.

[Cumulation and effect of chemokines and CD40L during blood storage--review].

Chemokine is a class of soluble active peptides that attract white blood cells to the inflammatory site. CD40 ligand (CD40L) involves in synthesis of proinflammatory mediators. Accumulation of chemokine and CD40L can induce non-hemolytic reaction after transfusion, transfusion-related acute lung injury (TRALI) and autoimmune disease during blood component storage.

Allelic diversity and haplotype structure of HLA loci in the Chinese Han population living in the Guanzhong region of the Shaanxi province.

The allele and haplotype frequencies of HLA-A, -B and -DRB1 loci in 10,000 healthy unrelated Han individuals living in the Guanzhong region of the Shaanxi Province were analyzed with the methods of SSO, SSP and SBT. Subsequently, these data were compared with results obtained in Han populations living in other regions as well as to other ethnic groups, using genetic distance measurements, neighbor-joining dendrograms and principal component analysis. In total 18 alleles were detected at the HLA-A locus, 46 alleles at the HLA-B locus and 14 alleles at the HLA-DRB1 locus.