A structure optimized fluorescent probe for highly sensitive monitoring drug induced lysosomal pH value changes.

Lysosomes generally maintain the weak acidic microenvironment, to ensure highly efficient activity and functions of hydrolytic enzymes and proteins. Aberrations of the lysosomal pH may result in cellular functional changes and influence human physiology, possibly causing serious diseases. Small-molecular fluorescent probes based imaging techniques capable of providing information on target locations are considerably appreciated.

A novel two-photon fluorescent probe with long-wavelength emission for monitoring HClO in living cells and tissues.

Hypochlorous acid (HClO), one of the most important reactive oxygen species (ROS), is a potent antimicrobial agent for the immune system against invasive bacteria and a wide range of pathogens. Therefore, it is critical to develop sensitive and selective methods for visualization of HClO in biological samples. In this work, a two-photon fluorescent probe HN2-TP) with long-wavelength emission (far-red: 630 nm) based on rhodamine analogue for bioimaging HClO was developed.