Strategies for plant-microbe symbiosis: Mycorrhizal fungi and helper bacteria to improve cold tolerance in rice.

Cold stress is a limiting factor for rice yield. Empirical evidence has demonstrated that arbuscular mycorrhizal fungi (AMF) can bolster the cold resilience of plants. In barren environments, AMF can promote host plant growth and resistance.

Patient-Derived Organoids for Guiding Neoadjuvant Chemotherapy in Bilateral Primary Breast Cancer: A Case Report.

Neoadjuvant chemotherapy (NAC) is the standard of care for locally advanced breast cancer, but the response to chemotherapy is unpredictable due to unknown information on the tumor drug sensitivity. Patient-derived organoids (PDOs) have recently been revealed to be a promising platform for assessing drug sensitivity in many cancers. A 46-year-old woman presented to the hospital due to an accidentally discovered mass in the left breast.

Melatonin Increased Autophagy Level to Facilitate Osteogenesis of Inflamed PDLSCs Through TMEM110 Signaling Pathways.

Periodontal ligament stem cells (PDLSCs) bring new hope to patients with poor periodontium recovery and impaired regeneration. However, the complex inflammatory microenvironment continually inhibits stem cell function and hinders stem cell therapy effectiveness. Melatonin is a naturally occurring neurohormone that participates in the regulation of a large spectrum of biological functions.

Distribution and accumulation dynamics of fungicide azoxystrobin in the soil-plant system.

Azoxystrobin is a widely used fungicide belonging to the strobilurin group, primarily employed to control a range of fungal diseases in crops, including pepper (Capsicum annuum L.). As a systemic pesticide, it effectively inhibits mitochondrial respiration in fungi, thereby preventing their growth and spread.

Discovery of a novel 2,4-thiazolidinedione derivative as dual inhibitor of β-catenin/TCF4 interaction and tubulin polymerization in colon cancer cells.

Hyperactivation of the Wnt/β-catenin signaling pathway has been widely recognized as a pathogenic mechanism for colorectal cancer (CRC). Based on a previously reported lead compound, iCRT14, a series of 2,4-thiazolidinedione derivatives were designed, synthesized, and evaluated in vitro for their antiproliferative activity against colon cancer cells. Compound 15k exhibited the most potent activity against the HCT116 and SW480 cell lines.

Constructing a Nursing Programme to Manage Intra-Abdominal Pressure in Neurocritical Care Patients: A Modified Delphi Study.

Aim: To construct a nursing programme to manage intra-abdominal pressure (IAP) in neurocritical care patients.

Design: The consensus of 16 experts was collected using a two-round Delphi method.

Methods: First, we developed the early stages of the nursing programme for managing IAP in neurocritical care patients through preliminary study and relevant literature internationally.

Cross-Modal Conditioned Reconstruction for Language-guided Medical Image Segmentation.

Recent developments underscore the potential of textual information in enhancing learning models for a deeper understanding of medical visual semantics. However, language-guided medical image segmentation still faces a challenging issue. Previous works employ implicit architectures to embed textual information.

Targeting leukocyte immunoglobulin‑like receptor B2 in the tumor microenvironment: A new treatment prospect for solid tumors (Review).

Leukocyte immunoglobulin-like receptor B2 (LILRB2) functions as an immunosuppressive receptor that has a prominent role in immune regulation. The expression of LILRB2 is higher in a variety of solid malignant tumors compared with that in corresponding normal tissues. LILRB2 can be expressed in tumor cells and tumor stromal cells within the tumor microenvironment.

[Effects of Electromagnetic Pulses on Exosomes Secretion by A549 Cells].

Background: Numerous researches indicated that electromagnetic pulses (EMP) possessed advantages such as strong targeting, minimal side-effects and low treatment cost in tumor therapy, but its optimum parameters for treatment and the relationship between EMP and tumor-derived exosomes remains unclear. This study aims to clarify the effects of EMP with different parameters on the quantity and miRNA (microRNA) of exosomes secreted by human non-small cell lung cancer A549 cells, providing beneficial reference for the clinical application of EMP and related research.

Methods: A549 cells were randomly divided into control group and different EMP radiation groups with respective intensity of 400, 600 and 800 kV/m.

Simultaneous removal of ammonia, cadmium, and oxytetracycline via a double-layer immobilized bioreactor driven by manganese redox: Optimization and potential mechanism.

The coexistence of ammonia nitrogen (NH-N), heavy metals and antibiotics in composite polluted wastewater has garnered significant attention. This study developed a novel double-layer biological carrier using sodium alginate, diatomite, polyvinyl alcohol, manganese-modified biochar, and pyrolusite, loaded with strains YZ8 and MA23 to form an efficient bioreactor (M1). Under conditions of a hydraulic retention time of 24 h, the carbon to nitrogen ratio and pH were 1.

Lab on a single microbead: An enzyme-free strategy for the sensitive detection of microRNA via efficient localized catalytic hairpin assembly.

Background: Accurate quantification of microRNA (miRNA) is of great significance because it provides opportunities for the accurate early diagnosis of a series of human diseases including cancers. Currently, complicated nucleic acid amplification technologies are always required for the highly sensitive miRNA detection. The introduction of nucleic acid signal amplification coupled with various enzymes will inevitably lead to tedious work and increase the complexity of the analysis process.

Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR.

We present a cost-effective and simple multiplex nucleic acid quantification method using droplet digital PCR (ddPCR) with digital melting curve analysis (MCA). This approach eliminates the need for complex fluorescent probe design, reducing both costs and dependence on fluorescence channels. We developed a convolutional neighborhood search algorithm to correct droplet displacement during heating, ensuring precise tracking and accurate extraction of melting curves.

Effect of Canna edulis starch addition on the properties of flour, rheology of dough and quality of semi-dry noodles.

Background: Canna edulis is a high-quality resistant starch raw material, especially for making flour products. This study aimed to investigate the effect of Canna edulis starch (CES) on the properties of flour, rheology of dough and quality of semi-dry noodles. The CES replaced part of the wheat flour in the semi-dry noodle formula.

A machine learning-based model for predicting the risk of cognitive frailty in elderly patients on maintenance hemodialysis.

Elderly patients undergoing maintenance hemodialysis (MHD) face a heightened risk of cognitive frailty (CF), which significantly compromises quality of life. Early identification of at-risk individuals and timely intervention are essential. Nevertheless, current CF risk prediction models fall short in accuracy to adequately fulfill clinical requirements.

Peptide-functionalized gold nanostars-assisted single-cell Raman spectra for molecular analysis of tumor metastasis based on cancer stem cells.

Cancer is one of the most fatal diseases threatening public health globally, and tumor metastasis causes greater than 90 % of cancer-associated deaths, presenting huge challenges for detection and efficient treatment of various human cancers. Cancer stem cells (CSCs) are a rare population of cancer cells and increasing evidences indicated CSCs are the driving force of tumor metastasis. In this study, a p-AuNSs-assisted single-cell Raman spectra has been established, to extract and amplify of CSCs fingerprints with single cell sensitivity.

Fecal occult blood is associated with an increased risk of cerebral small vessel disease in elderly patients.

Background And Purpose: Studies have shown that fecal occult blood is associated with an increased risk of ischemic stroke, but the relationship between fecal occult blood and cerebral small vessel disease (CSVD) remains largely unknown. This study aimed to identify predictors for CSVD, with fecal occult blood and its proxies as potential influencing factors in elderly patients.

Method: Patients aged 65 years or older with various chronic diseases were enrolled.

Simultaneous removal of ammonia, copper ions and sulfamethoxazole from aquaculture wastewater with low carbon to nitrogen ratio enhanced by manganese redox driven by a two-stage synergistic bioreactor: Optimization and potential mechanism.

The problem of low carbon-nitrogen ratio (C/N) in wastewater is a major challenge for biological treatment, especially the complex pollution of ammonia nitrogen (NH-N), sulfamethoxazole (SMX), and copper ions (Cu(II)). Herein, a strain of Pseudoxanthomonas sp. MA23 with manganese (Mn) reduction-coupled ammonia oxidation properties was isolated.

Evaluating sagittal condylar inclination: a comparative analysis of various digital workflow measures.

Introduction: This study aims to compare sagittal condylar inclination (SCI) measurements derived from three workflows: intraoral scan (IOS) aligned with cone-beam computed tomography (CBCT), IOS aligned with facial scan (FS), and a jaw motion analyzer (JMA) system, in a cohort of young individuals with established normal occlusion. Additionally, the study aims to identify sources contributing to variance in these measurement approaches.

Methods: Twenty-four healthy individuals exhibiting normal occlusion were enrolled in this clinical trial.

Hypoxia induced cellular and exosomal RPPH1 promotes breast cancer angiogenesis and metastasis through stabilizing the IGF2BP2/FGFR2 axis.

Metastasis is the major cause of breast cancer mortality, with angiogenesis and tumor-released exosomes playing key roles. However, the communication between breast cancer cells and endothelial cells and its role in tumor metastasis remains unclear. Here, we characterize a long noncoding RNA, RPPH1, which is upregulated in breast cancer tissues and positively associated with poor prognosis.

Coordination-Induced Magnetism Strategy for Highly Selective and Efficient Uranium Separation.

Highly efficient separation of dispersed uranium is important for the sustainable development of nuclear industry, and adsorption is the most recognized approach. However, there are many coexisting interfering metal ions that compete with uranyl ion for the chelating ligands in the adsorbents and lead to low separation selectivity and efficiency. Herein, a coordination-induced magnetism strategy is presented for the separation of uranium based on the conversion of diamagnetic cyanoferrocene (Fc-CN) nanocrystals to uranium-containing magnetic recoverable ferromagnetic aggregates.

Development of Stable and Intensified Mixing Processes for the Precise and Scalable Production of Uniform Drug Delivery Nanocarriers.

Nanocarriers show great promise in drug delivery but face challenges in stability, uniformity, and morphology control. This work introduces an enhanced mixing process to overcome these obstacles, specifically aiming to produce consistently sized poly(lactic-co-glycolic) acid (PLGA) nanoparticles loaded with anti-tumor drugs. By innovatively integrating a pulsation dampener into the microfluidic channels of a continuous flow preparation system, the flow stability of piston pumps is improved nearly tenfold.

Optimization and characterization of alginate/cinnamaldehyde electrosprayed microcapsule and its application to mongolian cheese preservation.

alginate is a good candidate for encapsulating bioactive compounds because the Na on its carboxyl groups can take part in an ion exchange process with Ca to generate a calcium alginate shell. Electrospraying technology was used to prepare cinnamaldehyde (CA)-loaded alginate microcapsules. The generation of microcapsules with a minimal diameter could improve the mass transfer of encapsulated materials.

Dynamic change and risk factors of intense thirst in patients admitted to neuro-intensive care unit: An observational study.

Background: Thirst is a clinical discomfort symptom reported by most patients admitted to intensive care unit (ICU). Little is known about the dynamic change and risk factors of intense thirst in neuro-intensive care unit (Neuro-ICU).

Aim: The objective of this study was to explore the dynamic change and determine the risk factors of intense thirst in patients admitted to Neuro-ICU, providing reference for personalized interventions of intense thirst.