Light Regulates Secreted Metabolite Production and Antagonistic Activity in .

Secondary metabolism is one of the main mechanisms uses to explore and colonize new niches, and 6-pentyl-α-pyrone (6-PP) is an important secondary metabolite in this process. This work focused on standardizing a method to investigate the production of 6-PP. Ethanol and ethyl acetate were both effective solvents for quantifying 6-PP in solution and had limited solubility in potato-dextrose-broth media.

Molecular Modeling of Vasodilatory Activity: Unveiling Novel Candidates Through Density Functional Theory, QSAR, and Molecular Dynamics.

Cardiovascular diseases (CVD) pose a significant global health challenge, requiring innovative therapeutic strategies. Vasodilators, which are central to vasodilation and blood pressure reduction, play a crucial role in cardiovascular treatment. This study integrates quantitative structure- (QSAR) modeling and molecular dynamics (MD) simulations to predict the biological activity and interactions of vasodilatory compounds with the aim to repurpose drugs already known and estimateing their potential use as vasodilators.

Carbon and Nitrogen Sources Influence Parasitic Responsiveness in NI-1.

Parasitic species of use hydrolytic enzymes to destroy the host cell wall. Preferent carbon and nitrogen sources suppress the expression of genes related to parasitism. Here, different nutrients were evaluated in the parasitic isolated NI-1, which was identified as .

Uncovering the multifaceted properties of 6-pentyl-alpha-pyrone for control of plant pathogens.

Some volatile organic compounds (VOCs) produced by microorganisms have the ability to inhibit the growth and development of plant pathogens, induce the activation of plant defenses, and promote plant growth. Among them, 6-pentyl-alpha-pyrone (6-PP), a ketone produced by fungi, has emerged as a focal point of interest. 6-PP has been isolated and characterized from thirteen species and is the main VOC produced, often accounting for >50% of the total VOCs emitted.

Oral Habits in Childhood and Occlusal Pathologies: A Cohort Study.

Purpose: To analyse the relationship between the different habits that occur in childhood and the different malocclusions in the three planes of space.

Material And Methods: A clinical examination of 106 children between 5 and 12 years of age was carried out and a survey validated by professors of the Faculty of Dentistry of the University of Seville was made for the parents in order to identify the habits and relate them to the possible malocclusions detected in the child's mouth.

Results: 72.

Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 in .

Functional microexons have not previously been described in filamentous fungi. Here, we describe a novel mechanism of transcriptional regulation in requiring the inclusion of a microexon from the gene. In low-glucose environments, a long mRNA including the microexon encodes a protein with a GAL4-like DNA-binding domain (Xlr2-α), whereas in high-glucose environments, a short mRNA that is produced encodes a protein lacking this DNA-binding domain (Xlr2-β).

Comparison of industrial engineering programs in Colombia based on standardized test results.

This study aims to evaluate the performance of industrial engineering programs across various Higher Education Institutions (HEIs) in Colombia, focusing on the SaberPRO test outcomes from 2016 to 2018. The evaluation considers three primary dimensions: the type of funding (public vs. private HEIs), geographical region, and program accreditation status.

Ecological functions of fungal sesquiterpenes in the food preference and fitness of soil Collembola.

Volatile organic compounds (VOCs) emitted by fungi play a key role in locating and selecting hosts for fungivorous arthropods. However, the ecological functions of many common VOC classes, such as sesquiterpenes, remain unknown. Mutants of , defective in the emission of most sesquiterpenes owing to the deletion of the terpene cyclase , were used to evaluate the role of this compound class in the food preference and fitness of the soil Collembola Choice experiments with and without direct contact with fungal mycelium revealed that Collembola were preferentially attracted to mutants impaired in sesquiterpene synthesis compared to wild-type .

Longevity of Aesthetic Fixed Space Maintainers in the Anterior Area of the Pediatric Dental Patient.

Unlabelled: Space maintenance for children at very early ages in primary molars or posterior sectors is widely used and the scientific evidence is clear as to its indications. In the anterior sectors there are doubts as to whether there is a loss of space and its use is usually accompanied by aesthetic, phonatory requirements or the completion of certain habits. In this type of aesthetic anterior maintainer, there are many medium and long-term complications that can occur.

Osmotic Stress Responses, Cell Wall Integrity, and Conidiation Are Regulated by a Histidine Kinase Sensor in .

responds to various environmental stressors through the mitogen-activated protein kinase (MAPK) Tmk3 and MAPK-kinase Pbs2 signaling pathways. In fungi, orthologues to Tmk3 are regulated by a histidine kinase (HK) sensor. However, the role of HKs remains unknown.

Dual role of endophytic entomopathogenic fungi: induce plant growth and control tomato leafminer Phthorimaea absoluta.

Background: Entomopathogenic fungi (EPF) are multifunctional microorganisms acting not only as biopesticides against insect pests but also as endophytes which regulate plant growth. The tomato leafminer, Phthorimaea absoluta (Tuta absoluta) is a devastating invasive pest of tomatoes worldwide. However, effective alternatives are needed for a sustainable management of this invasive pest.

Relationship between the TGFBR1 Gene and Molar Incisor Hypomineralization.

Background: Molar Incisor Hypomineralization Syndrome (MIH) is a problem of increasing incidence that represents a new challenge in the dental treatment of many of the children we see in our dental offices. Understanding the etiology of this syndrome (still unknown) will help us to prevent the appearance of this process. Lately a certain genetic relationship has been suggested in the syndrome.

Classification of industrial engineering programs in Colombia based on state tests.

This article proposes an approach for the classification of industrial engineering programs offered by different higher education institutions (HEIs) in Colombia, using data envelopment analysis (DEA) and validating the results with cluster analysis. To perform this classification, data from 5318 industrial engineering students from 93 higher education institutions are used as a basis for classification based on the Saber11 and SaberPro state tests. The state tests are used to measure graduates' academic performance in the data envelopment analysis.

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in sp. " B".

spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different species is well documented, information on intraspecific variation is limited.

Relationship between Children's Lifestyle and Fear during Dental Visits: A Cross-Sectional Study.

The purpose was to analyze the relationship between new family and social patterns and child emotions in the dental clinic. The sample consisted of 174 children between the ages of four and nine. Parents completed 20 questions that referred to social, family, cultural, and economic factors.

Knowledge of First Aid Measures in Dental Trauma: A Survey of Teachers in the Province of Seville, Spain.

The high incidence of childhood dental trauma requires childcare professionals to have basic notions of dental first aid. The aim of this study is to assess the level of knowledge and aptitude (defined as the ability to operate competently in a given activity) of early childhood, primary, and/or secondary education teachers from Seville (Spain) referred to first aid measures in dental trauma. A descriptive study was conducted.

Histidine kinase two-component response regulators Ssk1, Skn7 and Rim15 differentially control growth, developmental and volatile organic compounds emissions as stress responses in Trichoderma atroviride.

The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutritional stress in fungi. In order to verify the involvement of these genes in IMI206040's growth, conidiation, direct antagonism against plant pathogens ( and ), production of volatile organic compounds (VOCs) with fungistatic effect, and interaction with plants (growth promotion), single mutants were generated, and the phenotypic patterns were analysed in comparison to the wild-type () strain. The mutants were submitted to osmotic, oxidative, membrane and cell wall stress conditions in vitro.

Radiographic differences observed following apexification vs revascularization in necrotic immature molars and incisors: a follow-up study of 18 teeth.

Purpose: To evaluate the effectiveness of apexification versus revascularization in the treatment of necrotic immature teeth and determine which strategy affords the greatest radiological success rate.

Methods: An analysis was made of 18 teeth subjected to mineral trioxide aggregate (MTA) apical plugging and regenerative endodontic treatment, assessing healing of the apical lesions and the changes in root dimensions.

Results: Significantly greater root growth was observed with revascularization in terms of the percentage change in length (12.

Insights into Metabolic Changes Caused by the -Maize Root Interaction.

The interactions of crops with root-colonizing endophytic microorganisms are highly relevant to agriculture, because endophytes can modify plant resistance to pests and increase crop yields. We investigated the interactions between the host plant and the endophytic fungus at 5 days postinoculation grown in a hydroponic system. Wild-type and two knockout mutants, with deletion of the genes or involved in specialized metabolism, were analyzed.

TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Genes.

Species of the genus are ubiquitous in the environment and are widely used in agriculture, as biopesticides, and in the industry for the production of plant cell wall-degrading enzymes. represents an important genus of endophytes, and several species have become excellent models for the study of fungal biology and plant-microbe interactions; moreover, are exceptional biotechnological factories for the production of bioactive molecules useful in agriculture and medicine. Next-generation sequencing technology coupled with systematic construction of recombinant DNA molecules provides powerful tools that contribute to the functional analysis of genetics, thus allowing for a better understanding of the underlying factors determining its biology.

The NADPH Oxidases Nox1 and Nox2 Differentially Regulate Volatile Organic Compounds, Fungistatic Activity, Plant Growth Promotion and Nutrient Assimilation in .

In eukaryotic systems, membrane-bound NADPH oxidases (Nox) generate reactive oxygen species (ROS) as a part of normal physiological functions. In the soil-borne mycoparasitic and plant facultative symbiont , Nox1 and the regulator NoxR are involved in differentiation induced by mechanical damage, while the role of Nox2 has not been determined. The knock-out strains Δ, Δ and Δ were compared to the parental strain (WT) in their ability to grow and conidiate under a series of stress conditions (osmotic, oxidative, membrane, and cell-wall stresses).

[Allergies in children due to early food consumption in Barranquilla, Colombia].

Objective: To determine the prevalence of symptoms associated with asthma, rhinitis and eczema with food intake in children under six years of age.

Materials And Methods: Descriptive study on the prevalence of asthma, rhinitis and eczema and their correlation with food consumption. Information was obtained from 1 520 schoolchildren of the District of Barranquilla and its metropolitan area.