Establishing a standardized murine orthotopic intra-rectal model for the study of colorectal adenocarcinoma.

Background: Orthotopic models offer a more accurate representation of colorectal cancer (CRC) compared to subcutaneous models. Despite promising results from the reported intra-rectal models, establishing a standardized method for CRC research remains challenging due to model variability, hindering comprehensive studies on CRC pathogenesis and treatment modalities, such as brachytherapy. This study aimed to establish a standardized workflow for an orthotopic intra-rectal animal model to induce the growth of colorectal adenocarcinoma in male and female mice.

Investigation of dosimetric characteristics of radiochromic film in response to alpha particles emitted from Americium-241.

Background: In radiotherapy, it is essential to deliver prescribed doses to tumors while minimizing damage to surrounding healthy tissue. Accurate measurements of absorbed dose are required for this purpose. Gafchromic external beam therapy (EBT) radiochromic films have been widely used in radiotherapy.