Fatigue failure load of zirconia-reinforced lithium silicate glass ceramic cemented to a dentin analogue: Effect of etching time and hydrofluoric acid concentration.

This study aimed to evaluate the effect of etching time and hydrofluoric acid (HF) concentration on the fatigue failure load and surface characteristics of zirconia-reinforced lithium silicate glass (ZLS) ceramic cemented to a dentin-like, fiber reinforced epoxy resin. Ceramic (Suprinity, VITA) (1.0mm thick) and epoxy resin (2.

Transglutaminase from newly isolated Streptomyces sp. CBMAI 1617: Production optimization, characterization and evaluation in wheat protein and dough systems.

The popularity of transglutaminase (TG) by the food industry and the variation in functionality of this enzyme from different origins, prompted us to isolate and evaluate a high-yielding TG strain. Through the statistical approaches, Plackett-Burman and response surface methodology, a low cost fermentation media was obtained to produce 6.074±0.

Eosinophils release extracellular DNA traps in response to Aspergillus fumigatus.

Background: Eosinophils mediate the immune response in different infectious conditions. The release of extracellular DNA traps (ETs) by leukocytes has been described as an innate immune response mechanism that is relevant in many disorders including fungal diseases. Different stimuli induce the release of human eosinophil ETs (EETs).

Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging.

The pathology of schistosomiasis mansoni, a neglected tropical disease of great clinical and socioeconomic importance, results from the parasite eggs that become trapped in host tissues, particularly in the liver and intestines. Continuous antigenic stimulation from these eggs leads to recruitment of inflammatory cells to the sites of infection with formation of periovular granulomas. These complex structures have variable size and composition and are the most striking histopathological feature of schistosomiasis mansoni.

Oxidation of clofibric acid in aqueous solution using a non-thermal plasma discharge or gamma radiation.

In this work, we study degradation of clofibric acid (CFA) in aqueous solution using either ionizing radiation from aCo source or a non-thermal plasma produced by discharges in the air above the solution. The results obtained with the two technologies are compared in terms of effectiveness of CFA degradation and its by-products. In both cases the CFA degradation follows a quasi-exponential decay in time well modelled by a kinetic scheme which considers the competition between CFA and all reaction intermediates for the reactive species generated in solution as well as the amount of the end product formed.

Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts.

This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used.

SpotOn: High Accuracy Identification of Protein-Protein Interface Hot-Spots.

We present SpotOn, a web server to identify and classify interfacial residues as Hot-Spots (HS) and Null-Spots (NS). SpotON implements a robust algorithm with a demonstrated accuracy of 0.95 and sensitivity of 0.

Ostracoda and foraminifera from Paleocene (Olinda well), Paraíba Basin, Brazilian Northeast.

Paleocene ostracods and planktonic foraminifera from the Maria Farinha Formation, Paraíba Basin, are herein presented. Eleven ostracod species were identified in the genera Cytherella Jones, Cytherelloidea Alexander, Eocytheropteron Alexander, Semicytherura Wagner, Paracosta Siddiqui, Buntonia Howe, Soudanella Apostolescu, Leguminocythereis Howe and, probably, Pataviella Liebau. The planktonic foraminifera are represented by the genera Guembelitria Cushman, Parvularugoglobigerina Hofker, Woodringina Loeblich and Tappan, Heterohelix Ehrenberg, Zeauvigerina Finlay, Muricohedbergella Huber and Leckie, and Praemurica Olsson, Hemleben, Berggren and Liu.

An overview of Bacillus proteases: from production to application.

Proteases have a broad range of applications in industrial processes and products and are representative of most worldwide enzyme sales. The genus Bacillus is probably the most important bacterial source of proteases and is capable of producing high yields of neutral and alkaline proteolytic enzymes with remarkable properties, such as high stability towards extreme temperatures, pH, organic solvents, detergents and oxidizing compounds. Therefore, several strategies have been developed for the cost-effective production of Bacillus proteases, including optimization of the fermentation parameters.

New Heterofunctional Supports Based on Glutaraldehyde-Activation: A Tool for Enzyme Immobilization at Neutral pH.

Immobilization is an exciting alternative to improve the stability of enzymatic processes. However, part of the applied covalent strategies for immobilization uses specific conditions, generally alkaline pH, where some enzymes are not stable. Here, a new generation of heterofunctional supports with application at neutral pH conditions was proposed.

Erratum to: Antimicrobials: a global alliance for optimizing their rational use in intra-abdominal infections (AGORA).

[This corrects the article DOI: 10.1186/s13017-016-0089-y.].

Intrinsic and Extrinsic Aspects on Biofilms.

Biofilm represents a way of life that allows greater survival of microorganisms in hostile habitats. is able to form biofilms and on surfaces at several points in the poultry production chain. Genetic determinants related to their formation are expressed differently between strains and external conditions are decisive in this respect.

An Innovative Streaming Video System With a Point-of-View Head Camera Transmission of Surgeries to Smartphones and Tablets: An Educational Utility.

Purpose: In order to engage medical students and residents from public health centers to utilize the telemedicine features of surgery on their own smartphones and tablets as an educational tool, an innovative streaming system was developed with the purpose of streaming live footage from open surgeries to smartphones and tablets, allowing the visualization of the surgical field from the surgeon's perspective. The current study aims to describe the results of an evaluation on level 1 of Kirkpatrick's Model for Evaluation of the streaming system usage during gynecological surgeries, based on the perception of medical students and gynecology residents.

Methods: Consisted of a live video streaming (from the surgeon's point of view) of gynecological surgeries for smartphones and tablets, one for each volunteer.

The contrast between alveolar and velar stops with typical speech data: acoustic and articulatory analyses.

Purpose: This study used acoustic and articulatory analyses to characterize the contrast between alveolar and velar stops with typical speech data, comparing the parameters (acoustic and articulatory) of adults and children with typical speech development.

Methods: The sample consisted of 20 adults and 15 children with typical speech development. The analyzed corpus was organized through five repetitions of each target-word (/'kap ə/, /'tapə/, /'galo/ e /'daɾə/).

Gait performance of children and adolescents with sensorineural hearing loss.

Background: Several studies have demonstrated that children with sensorineural hearing loss (SNHL) may exhibit balance disorders, which can compromise the gait performance of this population.

Objective: Compare the gait performance of normal hearing (NH) children and those with SNHL, considering the sex and age range of the sample, and analyze gait performance according to degrees of hearing loss and etiological factors in the latter group.

Method: This is a cross-sectional study that assessed 96 students, 48 NH and 48 with SNHL, aged between 7 and 18 years.

Can Cleansing Regimens Effectively Eliminate Saliva Contamination from Lithium Disilicate Ceramic Surface?

This study evaluated the effect of cleaning protocols on the bond strength of resin cement to glass-ceramic. Ceramic specimens (N=120, n=12 per group) were etched with hydrofluoric acid and rinsed with water. After saliva contamination, specimens were cleaned as follows: water, 37% H3PO4, cleaning-paste (Ivoclean), or isopropanol.

The effects of 12 weeks Pilates-inspired exercise training on functional performance in older women: A randomized clinical trial.

Background: Recent scientific evidence supports the benefits of Pilates exercises on postural balance and muscle strength of older persons. However, their effects on other aspects of physical fitness, which are also important for independent living in older age, are still unknown.

Objective: To investigate the effects of a 12-week Pilates-inspired exercise program on the functional performance of community-dwelling older women.

Fatigue limit of monolithic Y-TZP three-unit-fixed dental prostheses: Effect of grinding at the gingival zone of the connector.

Objectives: To determine the fatigue limits of three-unit monolithic zirconia fixed dental prosthesis (FDPs) before and after grinding of the gingival areas of connectors with diamond burs.

Material And Methods: FDPs were milled from pre-sintered blocks of zirconia simulating the absence of the first mandibular molar. Half of the specimens were subjected to grinding, simulating clinical adjustment, and all of them were subjected to glazing procedure.

BCR-ABL1 transcript types showed distinct laboratory characteristics in patients with chronic myeloid leukemia.

In chronic myeloid leukemia (CML) two main types of messenger RNA (e14a2 and e13a2) can be produced by BCR-ABL1 gene rearrangement. Due to conflicting results, the clinical value of these transcripts remains controversial. The aim of this study was to identify associations of e14a2 and e13a2 transcripts with laboratory variables and also the response to treatment.

Revisiting the human seminiferous epithelium cycle.

Study Question: Can all types of testicular germ cells be accurately identified by microscopy techniques and unambiguously distributed in stages of the human seminiferous epithelium cycle (SEC)?

Summary Answer: By using a high-resolution light microscopy (HRLM) method, which enables an improved visualization of germ cell morphological features, we identified all testicular germ cells in the seminiferous epithelium and precisely grouped them in six well-delimitated SEC stages, thus providing a reliable reference source for staging in man.

What Is Already Known: Morphological characterization of germ cells in human has been done decades ago with the use of conventional histological methods (formaldehyde-based fixative -Zenker-formal- and paraffin embedding). These early studies proposed a classification of the SEC in six stages.

Draft genome sequence of Streptomyces sp. strain F1, a potential source for glycoside hydrolases isolated from Brazilian soil.

Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp.

Evaluation of e-beam irradiation effects on the toxicity of slaughterhouse wastewaters.

Slaughterhouse industry produces large volumes of polluted wastewater, which cause negative impacts on the environment. The objective of this study was to assess the effect of electron-beam irradiation on the ecotoxicity of slaughterhouse effluents with absorbed doses up to 35 kGy. Two acute toxicity assays were applied to evaluate the efficiency of irradiation onto toxicity of wastewater.

Polymorphisms of Il-10 (-1082) and RANKL (-438) Genes and the Failure of Dental Implants.

. Genetic polymorphisms in certain cytokines and chemokines have been investigated to understand why some individuals display implant flaws despite having few risk factors at the time of implant. .