Scavenging properties of metronidazole on free oxygen radicals in a skin lipid model system.

Reactive oxygen species (ROS) play a vital role in the pathophysiology of the skin disease rosacea, a chronic, genetically-determined and UV-triggered disease, leading to facial redness and blemishes and exhibiting a deep impact on a patient's self-esteem and quality of life. ROS can cause oxidative damage to nucleic acids, sugars, proteins and lipids, thereby contributing to adverse effects on the skin. Metronidazole has been the first-line topical agent therapy for many years; nevertheless the mechanism of action is still not well understood.

Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adaptor function to tropoelastin.

Fibrillin-containing microfibrils in elastic and nonelastic extracellular matrices play important structural and functional roles in various tissues, including blood vessels, lung, skin, and bone. Microfibrils are supramolecular aggregates of several protein and nonprotein components. Recently, a large region in the N-terminal portion of fibrillin-1 was characterized as a multifunctional protein interaction site, including binding sites for fibulin-2 and -5 among others.

Mass spectrometric characterization of human skin elastin peptides produced by proteolytic digestion with pepsin and thermitase.

This study investigated peptides resulting from the digestion of human skin elastin with pepsin and thermitase. Characterization of the peptides was performed using two complementary mass spectrometric techniques; LC/ESI-ion trap and nano-ESI-qTOF MS. 155 different peptides were identified using a combined database based and de novo sequencing approach resulting in a total sequence coverage of 65.

In vitro availability of kaempferol glycosides from cream formulations of methanolic extract of the leaves of Melilotus elegans.

In Ethiopian traditional medicine, Melilotus elegans Salzm. ex Ser. (Leguminosae) is used for the treatment of haemorrhoids and lacerated wounds.

Dermal delivery of desmopressin acetate using colloidal carrier systems.

Recently, the transdermal route has received attention as a promising means to enhance the delivery of drug molecules, particularly peptides, across the skin. In this work, the skin penetration profiles of desmopressin acetate from a colloidal system (water-in-oil microemulsion) and an amphiphilic cream, a standard formulation, were determined using Franz diffusion cells and compared. In the case of the microemulsion, the total percentages of dose obtained from different skin layers(stratum corneum to subcutaneous tissue) were 3.

LC-MS determination of desmopressin acetate in human skin samples.

A sensitive, selective and accurate high-performance liquid chromatography-mass spectroscopy (LC-MS) assay for the determination of desmopressin acetate (1-deamino-8-D-arginine vasopressin, DDAVP) from human skin samples was developed and validated. Pieces of human breast skin were impregnated with DDAVP solutions and DDAVP was extracted with an optimum extraction procedure. The extracted solutions were then analyzed by a LC system, comprising of a Nucleosil C18 column (CC 125/2, 120-3) and a mobile phase of 0.

LC/ESI-MS analysis of two elastin cross-links, desmosine and isodesmosine, and their radiation-induced degradation products.

In this work, the effect of Fenton reaction on two elastin cross-linked amino acids, desmosine (DES) and isodesmosine (IDE), in the absence or presence of different wavelength radiations generated from artificial sources has been evaluated using LC/ESI-MS. Irradiation as well as incubation of DES or IDE solutions in the presence of Fe(2+) and H(2)O(2) resulted in products with m/z 497.1 and 481.