Publications by authors named "Melissa L Mercier"
Article Synopsis
- Identifying stable reference genes in non-model organisms like Thalassiosira pseudonana is essential for accurately quantifying gene expression using RT-qPCR, but this process is challenging due to variability in gene expression across different environmental conditions.
- In this study, researchers analyzed transcriptome data under various nutrient conditions and explored different methods, including literature searches and clustering techniques, to find genes with stable expression patterns.
- The analysis revealed 179 candidate reference genes, including actin and ubiquitin ligase, which may serve as reliable references for future studies, highlighting the usefulness of the analysis of sequence counts (ASC) method over k-means clustering for isolating stably expressed genes.
Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on the oceanic carbon cycle, there have been a number of studies focused on how diatoms respond to limiting macro and micronutrients such as iron and nitrogen.
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