Publications by authors named "Melissa K Boles"
Article Synopsis
- An accurate genome assembly is essential for analyzing functional genomics, and this study explores the use of sequencing to identify mutations on mouse Chromosome 11.
- Researchers re-sequenced over 14,000 annotated exons from 41 mutant mouse lines, discovering 59 genetic variants across 55 genes, with a significant portion located in coding sequences causing missense mutations.
- The findings suggest that large-scale sequencing can effectively pinpoint mutations, including those in conserved noncoding regions, highlighting the ongoing challenge of connecting genetic mutations to specific phenotypes in mammals.
Background: ENU-mutagenesis is a powerful technique to identify genes regulating mammalian development. To functionally annotate the distal region of mouse chromosome 4, we performed an ENU-mutagenesis screen using a balancer chromosome targeted to this region of the genome.
Results: We isolated 11 lethal lines that map to the region of chromosome 4 between D4Mit117 and D4Mit281.