Inaccuracies of the ISO 11731 Method for Environmental Validation of in Building Water Systems: Opportunities to Improve Sensitivity and Detect Viable but Non-Culturable .

Current environmental diagnostics for the detection of fail to detect viable but non-culturable , have sensitivity limitations and are time-consuming (10-14 days to results). The objective of this study was to compare detection results between the standard ISO 11731 and an innovative detection method that utilizes a hybrid methodology of traditional microbiology and molecular detection. In this study, four hundred and seventy-six (476) potable building water samples were analyzed with ISO 11731 and the novel method in parallel.

Water Management for Construction: Evidence for Risk Characterization in Community and Healthcare Settings: A Systematic Review.

Construction activities are a known risk contributing to the growth and spread of waterborne pathogens in building water systems. The purpose of the study is to integrate evidence for categorizing construction activity risk factors contributing to waterborne disease in community and healthcare settings, establish severity of such risk factors and identify knowledge gaps. Using a systematic review, the inclusion criteria were: 1) studies with disease cases suspected to be associated with construction activities and waterborne pathogens, and 2) active construction work described in a community or healthcare setting.

Inaccuracy in Legionella tests of building water systems due to sample holding time.

Most Legionella culture tests are performed on building water samples that have been shipped to analytical laboratories for analysis. Significant (≥ 1 log₁₀ unit) changes in results were observed in 52% of held samples (6 h or longer, ambient temperature) drawn from building water systems in a 42-sample initial survey. It was not practical to use the spread plate protocol for on-site "t = 0" cultures in a larger, more diverse survey of thousands of building water systems.