Publications by authors named "Melina Daniilidis"
Article Synopsis
- High-resolution NMR studies of membrane proteins are difficult due to size-related signal issues, but segmental isotope labeling can help simplify the process.
- A stabilized split-intein system allows for effective protein trans-splicing under challenging conditions, enhancing the ability to label integral membrane proteins.
- This method improves NMR analysis by producing high-quality spectra in detergent micelles and lipid nanodiscs, making it valuable for studying the structures and dynamics of various proteins.
Membrane mimetics are essential for structural and functional studies of membrane proteins. A promising lipid-based system are phospholipid nanodiscs, where two copies of a so-called membrane scaffold protein (MSP) wrap around a patch of lipid bilayer. Consequently, the size of a nanodisc is determined by the length of the MSP.
View Article and Find Full Text PDFGlycosylation mediated by UDP-dependent glycosyltransferase (UGT) is one of the most common reactions for the biosynthesis of small molecule glycosides. As glycosides have various biological roles, we characterized UGT genes from grapevine (Vitis vinifera). In silico analysis of VvUGT genes that were highly expressed in leaves identified UGT92G6 which showed sequence similarity to both monosaccharide and disaccharide glucoside-forming transferases.
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