Heterologous expression of azurin from in food-grade .

In this study, azurin, a bacteriocin with anticancer property, was produced by food-grade using the Nisin Controlled Gene Expression (NICE) System. In addition, the antibacterial and cytotoxic properties of recombinant azurin in the culture supernatant were also investigated. Azurin gene from was cloned into the pNZ8149 vector and the resulting recombinant DNA was transformed into food grade NZ3900.

Efficient expression of recombinant human telomerase inhibitor 1 (hPinX1) in Pichia pastoris.

PinX1 encoded by a remarkable tumor suppressor gene and located in human chromosome 8p23 is known as telomerase inhibitor. In recent years, this protein has been of interest as clinically tumor suppressor. Pichia pastoris expression system is preferred to produce heterologous proteins and is suitable for industrial and research purposes.

Nitric oxide: a novel inducer for enhancement of microbial lipase production.

The purpose of this study was to elucidate whether exogenous nitric oxide (NO) has a potential beneficial effect on lipase production capacity of some microorganisms. Sodium nitroprusside (SNP) was used as an exogenous NO donor in production medium. In comparison with the control (0 nM SNP), SNP concentrations from 10 to 100 nM induced lipase production in mesophilic bacterium Bacillus subtilis and cold-adapted yeast Yarrowia lipolytica.