DNA double-strand breaks and their repair in blood lymphocytes of patients undergoing angiographic procedures.

Objectives: To adapt gamma-H2AX immunofluorescence microscopy to assessment of induction and repair of DNA double-strand breaks (DSBs) in peripheral blood lymphocytes in patients undergoing angiographic procedures.

Materials And Methods: The study was approved by the institutional ethics committee. After written informed patient consents were obtained, venous blood samples were taken from 19 patients (age 23-88 years) undergoing different angiographic procedures before, during, and after (10 minutes-24 hours) the examination.