Publications by authors named "Melanie Widmann"
Article Synopsis
- Research has traditionally focused on male subjects when studying epilepsy, but recent attention has shifted towards female mice to better understand the condition.
- The intrahippocampal kainic acid (IHKA) model used in this study successfully replicated key features of temporal lobe epilepsy (TLE) found in males, including drug-resistant seizures in female mice.
- Antiseizure medications showed a limited effect on hippocampal paroxysmal discharges (HPDs), with diazepam being the only medication to significantly reduce seizure activity, highlighting the need for gender-specific approaches in epilepsy research and treatment.
In the quest for novel treatments for patients with drug-resistant seizures, poor water solubility of potential drug candidates is a frequent obstacle. Literature indicated that the highly efficient solvent dimethyl sulfoxide (DMSO) may have a confounding influence in epilepsy research, reporting both pro- and antiepileptic effects. In this study, we aim to clarify the effects of DMSO on epileptiform activity in one of the most frequently studied models of chronic epilepsy, the intrahippocampal kainic acid (IHKA) mouse model, and in a model of acute seizures.
View Article and Find Full Text PDFAntibodies are important tools for protein and peptide research, including for the kappa opioid receptor (KOR) and dynorphins (Dyns). Well-characterized antibodies are essential for rigorous and reproducible research. However, lack of validation of antibody specificity has been thought to contribute significantly to the reproducibility crisis in biomedical research.
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