Publications by authors named "Melanie Seiders"
Reliable, validated diagnostic tests are critical for rabies control in animals and prevention in people. We present a performance assessment and updates to the LN34 real-time RT-PCR assay for rabies diagnosis in postmortem animal brain samples. In two U.
View Article and Find Full Text PDFThe Pennsylvania Department of Health Bureau of Laboratories (PABOL) tested 6855 animal samples for rabies using both the direct fluorescent antibody test (DFA) and LN34 pan-lyssavirus reverse transcriptase quantitative PCR (RT-qPCR) during 2017-2019. Only two samples (0.03%) were initially DFA negative but positive by LN34 RT-qPCR.
View Article and Find Full Text PDFArticle Synopsis
- - Rabies is a serious disease that needs quick and accurate diagnosis to prevent its spread, with the direct fluorescent antibody (DFA) test currently being the standard method for post-mortem diagnosis.
- - The LN34 pan-lyssavirus real-time RT-PCR assay shows great promise for diagnosing rabies, offering high sensitivity and the ability to work with various tissue types, even those that are deteriorated.
- - In a study involving nearly 3,000 samples across several regions, the LN34 assay proved to be highly reliable, outperforming the DFA test in certain cases, revealing its potential for enhancing rabies diagnostics and surveillance efforts.