Growth and current production of mixed culture anodic biofilms remain unaffected by sub-microscale surface roughness.

Bioelectrochemical systems couple electricity demand/supply to the metabolic redox reactions of microorganisms. Generally, electrodes act not only as electron acceptors/donors, but also as physical support for an electroactive biofilm. The microorganism-electrode interface can be modified by changing the chemical and/or topographical features of the electrode surface.

Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria.

This work evaluated the use of a culture enriched in DMRB as a strategy to enrich ARB on anodes. DMRB were enriched with Fe(III) as final electron acceptor and then transferred to a potentiostatically-controlled system with an anode as sole final electron acceptor. Three successive iron-enrichment cultures were carried out.

Specific and efficient electrochemical selection of Geoalkalibacter subterraneus and Desulfuromonas acetoxidans in high current-producing biofilms.

Two different saline sediments were used to inoculate potentiostatically controlled reactors (a type of microbial bioelectrochemical system, BES) operated in saline conditions (35 gNaCl l(-1)). Reactors were fed with acetate or a mixture of acetate and butyrate at two pH values: 7.0 or 5.

High current density via direct electron transfer by the halophilic anode respiring bacterium Geoalkalibacter subterraneus.

In this study the characterization of Geoalkalibacter subterraneus is presented, which is a novel halophilic anode respiring bacterium (ARB) previously selected and identified in a potentiostatically controlled bioelectrochemical system (BES) inoculated with sediments from a salt plant. Pure culture electroactive biofilms of Glk. subterraneus were grown during chronoamperometric batch experiments at a graphite electrode poised at +200 mV (vs.

Fate of cadmium in activated sludge after changing its physico-chemical properties by thermal treatment.

The effect of thermal treatment of activated sludge on cadmium uptake was investigated in respect with potential modifications of floc surface properties. Soluble fraction biochemical composition and floc size measurements evidenced floc destructuration and the release of (in)organic ligands in solution. Characterization of sludge samples by potentiometric titrations and IR spectra showed the transfer of functional groups from particulate to soluble fraction as well as the higher availability of phosphate groups originating from cell membrane phospholipids after thermal treatment.