Publications by authors named "Melanie Chiandotto"
Zymosan induces NADPH oxidase activation in human neutrophils by inducing the phosphorylation of p47phox and the activation of Rac2: involvement of protein tyrosine kinases, PI3Kinase, PKC, ERK1/2 and p38MAPkinase.
Karama Makni-Maalej Mélanie Chiandotto Margarita Hurtado-Nedelec Samia Bedouhene Marie-Anne Gougerot-Pocidalo
Biochem Pharmacol
January 2013
Reactive oxygen species (ROS) production by the neutrophil NADPH oxidase plays a key role in host defense against pathogens, such as bacteria and fungi. Zymosan a cell-wall preparation from Saccharomyces cerevisiae is largely used to activate neutrophils in its opsonized form. In this study, we show that non-opsonized zymosan alone induced ROS production by human neutrophils.
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- Interleukin-10 (IL-10) has anti-inflammatory effects by reducing the functions of polymorphonuclear neutrophils (PMNs), specifically by limiting reactive oxygen species (ROS) production.
- The study aims to understand if IL-10 can inhibit the phosphorylation of p47(PHOX) induced by proinflammatory cytokines like GM-CSF and TNF-alpha, finding that IL-10 specifically inhibits GM-CSF-induced p47(PHOX) phosphorylation.
- Results indicate that IL-10 decreases ROS production by affecting the ERK1/2 signaling pathway, thus suggesting a mechanism by which IL-10 regulates NADPH oxidase activity during inflammation.