Accumulation of Ag(I) by Saccharomyces cerevisiae Cells Expressing Plant Metallothioneins.

The various applications of Ag(I) generated the necessity to obtain Ag(I)-accumulating organisms for the removal of surplus Ag(I) from contaminated sites or for the concentration of Ag(I) from Ag(I)-poor environments. In this study we obtained Ag(I)-accumulating cells by expressing plant metallothioneins (MTs) in the model . The cDNAs of seven MTs (AtMT1a, AtMT1c, AtMT2a, AtMT2b, AtMT3, AtMT4a and AtMT4b) and four MTs (NcMT1, NcMT2a, NcMT2b and NcMT3) fused to myrGFP displaying an -terminal myristoylation sequence for plasma membrane targeting were expressed in and checked for Ag(I)-related phenotype.

Superficial and Inner Examination of a Microwave-Irradiated Dental Acrylic Resin and Its Metal-Polymer Interface.

The aim of this study is to conduct an extended surface and cross-section characterization of a denture base acrylic resin subjected to 500, 650, and 750 W microwave irradiation for 2, 3, and 5 min to assess its morphological modifications. A commercial heat-cured powder was polymerized according to the manufacturer's specifications and distributed into 20 circular samples. A stainless-steel wire was partially embedded in half of the discs, in order to investigate the metal-polymer interface.