[Biological properties and phylogenetic relationships of tick-borne encephalitis virus (Flaviviridae, Flavivirus) isolates of siberian subtype isolated in the south of East siberia in modern period].

Introduction: Tick-borne encephalitis virus (TBEV) is medically most important representative of the same-name serogroup of genus Flavivirus (Flaviviridae). In the view of various researchers there are 3 to 5 TBEV subtypes, of them siberian being the most prevalent. The aim of the work is to compare the biological properties and to reveal phylogenetic relationships of large group of modern (2006-2019) TBEV isolates of siberian subtype from natural foci in southern East Siberia.

Analysis of effectivity of the tickborne encephalitis virus detection methods in ixodid ticks.

Tick-borne encephalitis (TBE) is transmissible viral disease widely common in temperate zone of Eurasia. ELISA and PCR are used for express identification of the vector's infection, but the results of the two methods often do not agree. Aim of the work is comparative analysis for TBE virus of Ixodid ticks from nature using complex of methods, including ELISA, PCR, and isolation of the virus in laboratory mice.

Evaluation of Vitamin D Metabolism in Patients with Type 1 Diabetes Mellitus in the Setting of Cholecalciferol Treatment.

In this prospective controlled study, we examined 25 adults with adequately controlled (HbA1c level < 8.0%) type 1 diabetes mellitus (T1DM) and 49 conditionally healthy adults, intending to reveal the diversity of vitamin D metabolism in the setting of cholecalciferol intake at a therapeutic dose. All patients received a single dose (150,000 IU) of cholecalciferol aqueous solution orally.

Tick-borne encephalitis virus isolates from natural foci of the Irkutsk region: clarification of the genotype landscape.

The Irkutsk region is the unique territory where all known subtypes of tick-borne encephalitis virus (TBEV) circulate. In the last years, the phenomenon of changes in TBEV subtypes (substitution of the Far-Eastern subtype by the Siberian one) was noted in some regions of the Russian Federation. The results of individual investigation of 11522 Ixodes persulcatus ticks and brain specimens from 81 small mammals collected in natural foci of the Irkutsk region during 2006-2014 are presented in the article.

[Electrocardiographic predictors of response to cardiac resynchronization therapy of heart failure in patients with dilated cardiomyopathy].

Unlabelled: The aim of our study was to identify electrocardiographic predictors of response to cardiac resynchronization therapy (CRT) in patients with dilated cardiomyopathy (DCM).

Methods: 46 patients with DCM (76% men; mean age 48.8+/-12 years; mean NYHA class 3.

[Ratio of the numbers of Ixodes persulcatus imagoes in the near-Baikal area with the use of different recording methods].

The data of estimating the number of Ixodes perculcatus by two methods (the number of ticks per flag-hour and that per flag-kilometer) in the near-Baikal area were analyzed. According to these values, the abundance of taiga ticks in individual calculations ranged from 0 to 394. The association between the time of passage and the number of collected ticks was positive and high significant.

[Assessment of current disinfectants to create a reserve in case of avian influenza outbreak].

As of now, the market of disinfectants is so huge that it is very difficult even for a professional to get his/her bearings in it. The present paper proposes a procedure for estimating the efficiency of disinfectants in reference to the set task--to eliminate the sequels of avian influenza. The disinfectants that are most efficient for storage as a reserve in case of the above infection have been determined.

[Assessment of the use of chlorine-containing solid disinfectants in case of avian influenza pandemic].

The paper gives an economic assessment of the present market of monochlorine-containing disinfectants. It analyzes the consumption of disinfectants in g per m2 for disinfection measures in case of avian influenza epidemic.

[Experience of information sharing between local healthcare management and regional Administration of Federal Service for Surveillance for Protection of Consumers Rights and Human Welfare in Sverdlovsk region during outbreak of Legionnaires' disease in town Verkhnyaya Pyshma in July-August 2007].

Issues of reliability, uniformity, and timeliness of reporting and sharing of information during outbreaks of infectious diseases are basic for establishment of tactics and strategy of organizational, antiepidemic, and clinical measures. Analysis of experience in sharing of information between medical care organization service and services ensuring sanitary-epidemiological welfare of population during outbreak of Legionnaires' disease in town Verkhnyaya Pyshma in July-August 2007 was performed in the article.

[Diagnostic role of different immunologic methods for laboratory diagnostics of legionellosis].

The aim of the study was a comparative analysis of diagnostic value of different laboratoty methods conducted on the basis of results of examination of patients during Legionnaires' disease outbreak in town Verkhnyaya Pyshma. Retrospective analysis of laboratory data from 74 patients with diagnosis of Legionnaires' disease was performed. Complex of laboratory methods was used (polymerase chain reaction (PCR), enzyme immunoassay (EIA), immunochromatography).

[Phase II clinical trial of neoadjuvant hormone therapy in comparison with chemotherapy of patients with breast cancer].

Data are presented on a randomized study (stage II) which was undertaken to assess the efficacy of neoadjuvant chemotherapy (doxorubicin+paclitaxel) vis-a-vis endocrine therapy with aromatase inhibitors (anastrazole or exemestane) in postmenopausal women with ER-positive and/or PgR-positive tumors. Preoperative neoadjuvant chemotherapy was well tolerated and showed similar rates of overall response as compared with the latter regimen.

[Detection of Borrelia DNA in the Borrelia burgdorferi sensu lato complex in the blood of patients with Ixodes tick-borne borrelios].

Borrelia DNA was detected by polymerase chain reaction in the blood of patients who had suffered from tick suction. DNA was revealed in 28.7 of the blood samples from patients with the erythematous form of Ixodes tick-born borrelioses (ITBB) and in 14.

[Influence of cooling and warming of the arm on the reciprocal 1a inhibition of flexor muscles of the forearm of man].

In electrophysiological investigation on the healthy people the cooling (24 degrees C) and warming (42 degrees C) of arm was studied for their effects on the reciprocal 1a inhibition of flexor muscle of forearm during condition stimulation of extensor. Was observed, that cool and warm temperature stimulation enhanced this inhibition in flexor muscle. The cooling was more effectively in this enhancing.

[Recurrent inhibition of the flexor muscle motoneurons of the human forearm under the effect of vibration and static muscle strain].

By use of H-reflex technique reflex flexor muscles motoneurons recurrent inhibition was studied for man forearm muscle. It was observed the reduce of this inhibition with the muscles strain. The vibration, acting to the strained muscle, showed a disappearance of inhibition.

[Neuronal changes in the dorsal motor nucleus of the vagus nerve during intervals of hypoxic exposures in animals with diabetes mellitus].

Changes of neurons of dorsal motor nucleus of nervus vagus were studied in adaptation to hypoxia, experimental diabetes mellitus and its correction by means of interrupted hypoxic effects. It was established previously that interrupted hypoxic training exerted stimulating effect on insulin synthesizing function of pancreas. As a result of the present study the increase of morphofunctional activity of neurons was found in all experimental series although it was greater manifested in animals with experimental diabetes mellitus who were subjected to actions of hypoxia.

[The development and certification of the Hepabest anti-HBc IgG test system for detecting antibodies to the core antigen of the hepatitis B virus].

The comparison of the newly developed "HepaBest anti-HBc IgG" assay system for the detection of hepatitis B virus (HBV) core antigen with the reference system "ImmunoComb HBc IgG" (Orgenics, France-Israel) and its approbation on 91 serum samples from patients in the Infectious Clinical Hospital were carried out. The coincidence rate of the results obtained with the use of the two assay systems was 87.9%.

[Comparative data on the tick-borne encephalitis virus infectiousness of hungry and satiated taiga ticks (based on the results of an immunoenzyme analysis)].

The results of individual investigation of 25,500 Ixodes persulcatus ticks from east Siberia are presented. The ticks were collected from grass, men, and animals before their sticking and after feeding at different intervals. The quantity of positive specimens was 6-11 times higher among the fed ticks hungry ones and averaged 10.

[The geographical distribution of genetic variants of the tick-borne encephalitis virus].

Geographic distribution of 185 tick-borne encephalitis (TBE) virus strains isolated in 8 physico-geographic areas and classified into six genetic variants was analysed. The strains of genetic variant I homologous to the Sophyin prototype strain were found to occur predominantly in the Far East and also frequently found in Western and North-Western parts of the East European plain. The vast territories from lake Baikal in the East to Ukraine in the West harbor mostly the strains significantly different from the Far-Eastern Sophyin strain.

[The specific reactivity of cDNA and deoxyoligonucleotide probes, complementary to the tick-borne encephalitis virus genome, with the RNA of strains of different geographical origins].

Hybridization experiments with RNA of 143 tick-borne encephalitis (TBE) virus strains isolated in different parts of the distribution area were used to study the reactivity of kDNA- and a set of 10 synthetic deoxyoligonucleotide probes. The kDNA probe under certain conditions was shown to hybridize with RNA of all the strains under study, and under other (strict) hybridization conditions did so selectively with a small number of strains. The capacity of oligonucleotide probes for hybridization with RNA of TBE virus strains varied from 12% to 100%.