Complex Interaction of Platelets, von Willebrand Factor and Leukocytes, in Whole Blood at High Shear Rates Is Mediated by Platelet GPIIb/IIIa Receptor.

We studied the contribution of von Willebrand factor (vWF) into blood cell adhesion to collagen-coated surfaces in whole blood of healthy volunteers. Adhesion of blood cells to collagen I was measured at shear rate of 2300 sec. The interaction of platelet GPIIb/IIIa receptor with vWF was blocked with monoclonal anti-GPIIb/IIIa antibodies.

Kinetics of Platelet Adhesion to Protein-Coated Surface in Whole Blood Samples at High Flow Rates.

We studied platelet adhesion to fibrinogen-coated surface in whole blood samples under conditions of high flow rates. The degree of platelet adhesion was evaluated by the intensity of laser light scattered from protein-coated optical surface with adhered platelets. The intensity of adhesion in whole blood samples at high flow rates was by 2.

Real-Time Recording of Platelet Adhesion to Fibrinogen-Coated Surface under Flow Conditions.

We present a testing system allowing real-time recording of the kinetics of platelet adhesion to fibrinogen-coated surface under flow conditions. The system consists of an optical flow chamber, semiconductor laser, two photodetectors, analog-to-digital converter, computer, and peristaltic pump. Platelet adhesion to fibrinogen-coated surface is recorded with two photodetectors and analyzed by the intensity of total internal reflection and scattered laser radiation at the boundary of the blood sample and fibrinogen-coated optical surface.

Relationship between the Level of Circulating CD45 Platelets and Development of Restenosis after Implantation of Drug-Eluting Stents to Patients with Coronary Heart Disease.

The study was carried out in 126 patients with stable angina pectoris, who underwent elective coronary artery stenting with drug-eluting stents and follow-up angiography within 6-12 months thereafter. Five significant risk factors of restenosis were identified by binary comparisons of different variables. The logistic regression equation that included the level of CD45-positive platelets, diabetes, small vessel stenting, number of simultaneously implanted stents in one patient, and lesion length demonstrates the highest level of prediction of in-stent restenosis (OR=22.

[Structural peculiarities of the wall of the vessels of the cerebral arterial circle in the area of bifurcations in people of different age].

The present study was conducted to determine the morphological and morphometric features of the arterial wall structure at the bifurcation of blood vessels of the cerebral arterial circle (CAC) of Willis in people of different age (from birth till 65 years). Material obtained from 80 people was stained with hematoxylin-eosin, Van Gieson stain, with orcein by Unna-Taenzer's method and with sudan. The proliferative activity of the cells in tunica intima and tunica media at the site of bifurcation of the internal carotid and basilar arteries was studied immunohistochemically using monoclonal antibodies against Ki-67.

Controlling light by light in a one-dimensional resonant photonic crystal.

We consider the interaction and stability of gap solitons in a one-dimensional, resonant, photonic crystal with a defect state produced by a linear localized mode, or by an incoherent pump. Soliton propagation is investigated using both an analytical treatment and the direct numerical integration of the two-wave Maxwell-Bloch equations. Our results demonstrate that the soliton can be trapped, reflected from, or tunnel through the defect state.

Gap soliton dynamics in a nonuniform resonant structure.

A model for the propagation of coherent pulses along a one-dimensional, resonantly absorbing Bragg grating that includes localized inhomogeneous population inversion at its center is presented. The long-range coupling between the optical field and resonant atoms allows for controllable trapping of a gap soliton by the local inversion, thus opening new opportunities for control of signal transmission and localization of light.

Soliton dynamics of symmetry-endowed two-soliton solutions of the nonlinear Schrodinger equation.

A comprehensive analysis is presented of the propagation of symmetry-endowed two-soliton solutions under the influence of various perturbations important in nonlinear optics. Thus, we begin by introducing the analytical expressions of these two-soliton solutions. Then, by considering perturbations which preserve the initial symmetry of the two-soliton solutions, the dependence of the soliton parameters on the propagation distance is determined by using an adiabatic perturbation method.

[Dynamics of ecological-biochemical characteristics of the sea ice in the coastal zone of the White sea].

The distribution of salinity, silicon and phosphorus contents, and hydrolytic enzyme activities along a sea-coast transect was studied in melted ice cores and water samples taken from under the ice cover in the periods of active ice formation and melting in the Kandalaksha Bay, White Sea. The species list of identified algae was compiled, which included 170 species and varieties (90% of them belonged to diatoms). Strong correlations were revealed between the salinity of water samples and the content of silicon, protease activity, and the species composition of algae.

Soliton generation in optical fibers for a dual-frequency input.

We analyze scenarios of soliton generation in an ideal fiber for an input that consists of either two in-phase or out-of-phase solitonlike optical pulses at different frequencies. In both cases the relationship between the structure of the emerging solitons and the frequency separation of the initial solitons is studied both analytically and numerically. Depending on the value of the frequency detuning, if the two initial solitons are in phase (symmetric input), two bound solitons with equal amplitudes (breather), a single soliton, or a pair of solitons, which have equal amplitudes and exhibit opposite velocities, can be generated.

[Ultrasound-controlled minimally invasive surgical interventions in abdominal abscesses].

Results of ultrasound-controlled minimally invasive surgical interventions (UAMISI) for abdominal abscesses are analyzed. 84 operations were performed in 72 patients with good results. Puncture method was used in 12 patients, drainage operation--in 58, endoscopy-guided puncture method--in 2 patients.

[Changes in tissue monoamine oxidase activity and resistance to acute hypoxia in rats during stress].

Male Wistar rats exposed to different stresses developed shifts in the brain and liver monoaminoxidase activity. In the so called "cognitive" stimulation, the activity was enhanced in the brain and reduced in liver. Mild stresses also enhanced the activity in the brain.

[The determination of the time of death by the content of free amino acids in the liver and lungs of cadavers using high-performance liquid chromatography].

High-performance liquid chromatography is proposed to be used for assessment of the time of death during a fortnight postmortem. The procedure consists in dynamic measurements of the concentrations of 20 free amino acids in cadaveric liver and lungs of subjects dead from mechanical injuries with or without ethanol in the blood at moderately high temperatures (18 to 23 C) and 40-60% humidity. A working diagram for determination of the time of death is offered and diagnostic microblocks consisting of 5 most informative and stable amino acids developed, permitting the diagnosis of the time of death with 95% reliability.

[The forensic medical assessment of light bodily injuries. 1].

The authors discuss the essence and the criteria of assessment of slight bodily injuries and subdivide such injuries into two categories: those involving short-term health disorders and involving no disorders of this kind. They propose to alter the relevant sections of the actual "Regulations of the Forensic Medical Assessment of the Severity of Bodily Injuries".

[An analysis of fatal poisonings by alcohol surrogates, household chemicals and drugs].

The incidence of lethal poisonings with alcohol surrogates, communal chemical articles, and drugs in various regions of the former USSR was studied. Quantitative characteristics of such poisonings are presented.

[The lysosomal system of the hepatocytes during the development of extrahepatic cholestasis (a quantitative analysis)].

Electron microscopic and morphometric studies of the lysosomal system of hepatocytes with quantitative, informative and dispersion analysis of electron micrographs in healthy rats and rats with extrahepatic cholestasis have revealed the stage-like character of changes of the reaction of the liver subcellular structures in the processes of development of the pathologic process. At the first stage (12h-3 days) in parallel with the symptoms of injury (dilation of the lumen of bile canals with dystrophic alterations of epitheliocytes microvilli, depletion of the cytoplasm by glycogen, appearance of lipid inclusions, accumulation of the bile pigments) the initiation of urgent reactions of compensation was observed (using part of primary lysosomes in heterophagic processes, activation of autophagocytosis) as well as the beginning of the formation of long-term reactions with a somewhat increased amount of primary lysosomes and greater amount of small primary forms. There occur normalization of the ratio of primary and secondary forms.

Coherent amplification of ultrashort solitons in doped fibers.

We present the results of computer simulation of coherent amplification of solitons in doped fibers and explain the first stage of an evolution of such solitons by means of a perturbation theory applied to the inverse scattering problem in two limiting cases, noncoherent and pure coherent amplification. We show that it is possible to get adiabatic amplification of solitons. In the case of noncoherent amplification the amplitude of the solitons increases exponentially and the pulse duration decreases exponentially.

[Classification of erythroblastic islets of the bone marrow and the study of their cellular composition].

Cell composition of erythroblastic islands (EI), normal and at the phase of stimulation and inhibition of erythropoiesis at modelling of heat adaptation has been studied on hemopoietic tissue of 108 rats. In the hemopoietic tissue, together with EI of the 1st, 2nd and 3d classes of maturity, that have in their composition correspondingly up to 8, from 9 up to 16 and above 16 nuclei-containing erythroid cells, there are involutive and reconstructing EI. The involutive EI are presented as nuclei-containing erythroid cells, that are not able to divide and are at late stages of differentiation: polychromatophilic and oxyphilic normoblasts, as well as reticulocytes.