[New ways of correcting metabolic acidosis in experimental periodontitis].

Established prevention of atrophy of osseous cloth alveolar rats jaws sprouts and correction of metabolic acidosis of mineral concentrate "VITA" attached to modeling parodontit by dint of creation in them of fortune compensated metabolic acidosis. Mineral concentrate realizes normalization redoksfortune, thiol-disulphid system, lowering of relations SH/SS-group, multiplies maintenance oxidized and lowers maintenance restored of nicotinamid copherments, normalize relation HA[symbol: see text]/HA[symbol: see text]H in osseous jaws cloth of experimental rats attached to modeling in them metabolic acidosis and parodontit in distinction from fortune of alkalosis. Mineral complex will normalize in rats maintenance of regulators and orientation of exchange processes in cloths attached to parodontit, whereat indicates lowering of activity key gluconeogenesis processes enzyme fructosodiphosphatasa of liver cloths, mucous gum and alveolar sprouts of jaws.

[Interrelation of fetal metabolism and late pregnancy in animals].

Parameters of were determined for acid-alkali state of blood, energy, nitrogen, protein and mineral metabolism in fetuses and animals at the last period of pregnancy. It was established that one can observe the state of compensated acidosis, intensification of glycolytic and inhibition ot aerobic processes in animals with late pregnancy; that is evidenced by changes in the content of glucose, the level and the value of correlation of the components of lactate-, malate- and glutamate dehydrogenase systems, activity of lactate dehydrogenase, alkali phosphatase, alanine- and aspartate aminotransferase as well as electrolyte composition of the organism tissues. Analogous changes in metabolism are observe in the fetus tissues in late antenatal period of development.

[Correction of disturbances in acid-base status and metabolism of metabolites of the oxidative-reductive reaction in rickets].

The disturbance of redox and acid-base state in the tissues of rats with experimental rickets and in clinics has been studied. It was shown that administration of namacit for the directed correction of the disturbance of homeostasis allows decreasing the doses of vitamin D.

[Correlation of acid-base equilibrium and glycolysis during muscle activity].

Interdependence between acid-base equilibrium of blood and accumulation of lactate depending on the intensity of exertion during swimming and individual reaction to the exertion has been analyzed when examining high-qualification sportsmen. Prognostication and optimization criteria of acidosis prophylaxis are suggested.

[The characteristics of nitrogen metabolism in the tissues of rabbits with ammonium toxicosis].

Disturbances of nitrogen metabolism under acute ammonium toxicosis have been studied in tissues of rabbit. A sharp increase of the ammonium content in the blood and tissues of the liver and kidneys is accompanied by an increase in the glutamine and glutamate level in all tissues. The level of urea nitrogen in the blood of rabbits increases.

[Electrolyte composition of cow and calf tissues in health and in acute digestive disturbances].

Sodium, potassium, calcium, magnesium, manganese, zinc and chloride contents in the blood plasma of calves at parturition have been established to correspond to their levels in blood of cows during their calving period. Iron and inorganic phosphorus contents in calves blood plasma appeared to increase and copper content to be lower in this period as compared to postnatal period. By the third day of postnatal ontogenesis sodium concentration in calves blood decreased, copper level increased and the rest indices of water-salts metabolism in calves and adult animals were alike.

[Acid-base status of the blood and adenine nucleotide metabolism in ruminant erythrocytes during early post-natal development].

It has been established that data of acid-base balance in cows' and newborn calves' blood closely correlate with physiological status of animals during their initial days of postnatal ontogenesis and depend on the peculiarities of metabolism in fetus and newborn organisms. Data on the acid-base balance in blood of newborn calves under diarrhea syndrome are discussed from the point of view of the influence of rehydration therapy and "Namacit" preparation on clinical status of animals.

[Lipid composition of plasma membranes of jejunum enterocytes in cattle of different age].

The lipid composition of plasma membranes (a brush margin and basolateral membranes) of enterocytes from the jejunum of cattle: adult animals (aged 1.5-3) and newborns (3-5 days) both healthy and suffering from diarrhea has been studied. Substantial differences are established in the content of phospholipids, cholesterol and fatty acids in the brush margin and basolateral membranes as well as in the both membrane fractions of the adult, newborn and sick animals.

[Adenosine triphosphatase from plasma membranes of cattle intestinal epithelium].

The activities of Na,K-, Ca,Mg- and Mg-ATPases in the membrane fractions of plasma membranes of intestinal enterocytes of cattle, brush border and basolateral membranes, were studied. The activities were estimated under conditions of alkaline phosphatase activity inhibition by theophylline to exclude the nonspecific hydrolysis of ATP as well as to establish the orientation of vesicles with the use of alamethicine. 98% of the Na,K-ATPase activity (0.

[Impairment of energy metabolism in rabbit tissues during ammonia toxicosis].

The studies are conducted on the model of grave toxicosis in vivo in rabbits. It is shown that an increase in the ammonia content in blood and tissues enhances the content of lactate, glutamate, oxaloacetate in the liver and kidneys of animals, decreases the level of pyruvate, alpha-ketoglutarate and malate in the liver tissue. The NAD+/NADH ratio in the cytoplasm of the liver and kidney cells decreases, the ratio of NADP-pairs in the cytoplasm and mitochondria of these tissues noticeably increases.

[Metabolic system of acid-base homeostasis in the human and animal body].

Peculiarities of carbohydrate, lipid, amino acid nucleotide metabolism, of the tricarboxylic acid cycle functioning and of the oxidative phosphorylation have been studied in man and animals under conditions of changes in the acid-base equilibrium (ABE) state in the organism. The results of studies are analyzed and generalized. Strictly defined peculiarities of changes in the mentioned aspects of metabolism depending on the ABE state in the organism are revealed.

[Changes in the activity of alkaline phosphatase and Na K ATPase in the membrane fractions of small-intestinal epithelium in the normal state and in diarrhea].

Activity of alkaline phosphatase (apical membrane) and Na+, K+-ATPase (basal membrane) is determined to decrease in the membrane fractions of intestinal epithelium in the diarrheic new born cattle. It is concluded that Na+, K+-ATPase of basal membranes play a fundamental role in Na+ loss by body with diarrhea.

[Isolation, purification and characteristics of brush border and basolateral membranes from cattle intestinal epithelium cells].

The surface membrane of cattle intestine epithelium cells is separated into vesicated membrane fractions of the brush border and of basolateral membranes. The brush border membrane fraction is deposited with centrifugation (15,000 g) and is localized in the layers of 45, 56.5 and 48% in the density gradient of sucrose (105,000 g).

[Seasonal changes in the levels of various metabolites and proteins in cow milk and colostrum].

The studies on lactating black-and-white cows have shown that the content of albumins as well as of lactate, ammonia and glutamate in colostrum and milk of cows is higher in winter and spring but the level of beta- and gamma-globulins, citrate and urea is lower than the analogous indices in cows in summer. The concentration of alpha-globulins, oxaloacetate, malate and pyruvate in colostrum and milk did not change with the season.

[Effect of carbonic acid on glutamate dehydrogenase interaction with NADH].

The method of fluorescent titration was used to study the effect of carbonic acid on the process of NADH binding with the purified preparation of glutamate dehydrogenase as well as on fluorescent properties of the above mentioned coenzyme. It is shown that in bicarbonate buffer at the alkaline values of pH there is a decrease in the maximal capacity of the binding sites of the enzyme with low affinity to co-enzyme. The capacity of high-affinity binding sites in this case is unchanged.

[The use of buttermilk as a source of phospholipids].

A method for obtaining phospholipids from butter-milk, a by-product of butter making is described. The method consists in coagulating the fat globule membranes with mineral or organic acid, separating the resulted precipitate by centrifugation or filtering, lipid extraction by organic solvents mixture and isolation of phospholipids from the extract by acetone treatment or by column chromatography. Butter-milk, a new source of biological membranes, is characterized by a low cost and accessibility.

[Characteristics of the electrolyte balance of the blood of healthy newborn calves and calves with diarrhea].

Cows and newborn (just after the birth, and on the 1st, 3rd, 5th and 10th days after it) calves were tested for the peculiarities of the electrolyte blood composition and acid-base balance under normal and diarrheic conditions. The values of Na+/K+, Cl-/HCO3-, Pn/Ca2+ ratios in the blood serum of sick animals remain unchanged, which testifies to the deep disturbances of the water-salt metabolism in tissues.

[Chemical composition of the membranes of fat globules of cow's milk].

The methods of microthin-layer chromatography on plates with silica gel and disc-electrophoresis in PAAG are used to determine the lipid and protein composition of fat globule membranes of cow milk on the first (foremilk) and the tenth day (milk) of lactation as well as of the buttermilk, a by-product of the technological processing of milk. Differences are found in the quantitative content of the basic classes of lipids and proteins in membranes of fat globules produced from foremilk and milk of cows. The membranes of buttermilk and milk fat globules are characterized by the identical qualitative and similar quantitative chemical composition, that permits using buttermilk as easily available and rich source of components from membranes of cow milk fat globules in the first place of phospholipids and sterols.

[A nomogram method for calculating the NAD(P)+/NAD(P)H ratio in cell compartments].

A new method to calculate the ratios of free NAD+/NADH and NADP+/NADPH [NAD(P)+/NAD(P)H] in the cytoplasm and mitochondria of cells by means of nomographs is suggested. The method permits estimating the redox state of the tissue with allowance for the content of metabolites in the dehydrogenase systems. The method may be used widely in the biochemical and medical practice.

[Changes in the levels of various substrates of nitrogen metabolism and tricarboxylic acid cycle during experimental acidosis in calves].

The effect of experimental metabolic acidosis and its correction for nitrogen and energy metabolism was studied in new-born calves. It was discovered that a change in the acid-base balance towards acidosis causes a sharp increase in "ammoniogenesis", urea formation and inhibition of the tricarboxylic acid cycle, which is also observed in calves suffering from dyspepsia with symptoms of acute diarrhea. Alongside with the use of therapeutic measures for treating dyspepsia of new-born calves, it is necessary to control the acid-base balance of blood in the calves and in case of revealing the acidosis state to use means of its correction.

[Effect of the mineral-ammonia preparations MP-15 and MP-7 on nitrogen metabolism in cows].

It has been studied how MP-15 and MP-7 preparations fed on to lactating cows affect the indices of acid-base equilibrium of their blood, nitrogen metabolism, milk yield and milk quality. It is shown that feeding on MP-15 and MP-7 preparations to cows of test groups has no negative effect on the acid-base equilibrium indices, decreases the ammonia concentration by 38.5 and 54.

[Characteristics of nitrogen metabolism in chickens with gout].

It is shown that the intensity of ammonium genesis, purines biosynthesis and redox processes increases in the organism of chicken with visceral gout. Sodium bicarbonate-correction of the acid-alkaline state of chickens is performed in order to normalize disturbances observed under gout.

[Mechanisms of formation and biological role of protein carbamates].

Experimental data are presented evidencing for the fact that tissue proteins under conditions in vivo are subjected to enzymic carboxylation with formation of carbaminic groups. In this case a charge variation in definite sites of protein molecule is observed, which specifies variation of the protein conformation and biological properties. Basic regularities of protein carbamate formation reactions are revealed with factors affecting their intensity enumerated.

[Effect of preparation MP-15 on the productivity and metabolism of lactating cows].

Activation possibilities of redox and biosynthetic processes are studied for lactic cows with their ration supplemented by mineral-ammonium preparation MII-15. In the blood of cows from the experimental group the amount of a number of glycolysis, biocarboxylic cycle, ammonium nitrogen exchange products as well as activity of overamination enzymes vary as compared to control, which testifies to the stimulation of redox processes and protein exchange in tissues. Application of the MII-15 preparation is accompanied by an increase in the lactic productivity of cows by 8-10% on the average.

[A new method correcting metabolic acidosis].

A new method is suggested for correction of metabolic acidosis. The method consists in a decrease of pH of the bicarbonate sodium solution used for intravenous injection down to the physiological value of pH in the blood at the expense of buffering the solution by CO2. Experimental data conforming the efficiency of the new method application are presented.