Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

Analysis of inferior venacavography before Greenfield filter placement.

The value of routine preoperative inferior venacavography through a femoral approach was assessed in 64 patients who required a Greenfield inferior vena cava filter. Nearly 11% of the patients had an abnormality, for example, duplication of the inferior vena cava, circumaortic renal vein, interrupted or discontinuous inferior vena cava, and so on, requiring an alternate approach to placement. No deaths or complications occurred upon recognition of the anatomic variant before filter placement.

Correlation of serum fructosamine activity in type I diabetic children.

The serum fructosamine assay is a new commercially available test designed to measure serum glycated protein as an index of glycemic control in diabetes. The test relies on the ability of glucose bound to protein with a ketamine linkage (fructosamine) to act as a reducing agent in alkaline solution. Serum fructosamine activity was studied in 61 Type I diabetic patients attending a 2-week American Diabetes Association sponsored diabetic camp for children.

Case report: Macroglobulinemia in a dog.

This report describes a case of macroglobulinemia in a six year old castrate male Collie cross dog with clinical signs of epistaxis, anemia, retinopathy and high serum viscosity. The highest total serum protein was 12 g/dl with approximately 60% monoclonal beta globulin. Proteinuria, Bence Jones protein and osteolytic lesions were not detected.