Effects of mannoprotein on the stability and in vitro digestion of cyanidin-3-glucoside.

Although mannoprotein (MP) is known to increase the stability of anthocyanins, the MP-anthocyanin interactions and structural changes induced by the same remain underexplored. To bridge this gap, this work examined the complexation of cyanidin-3-glucoside (C3G) by MP and probed its effect on C3G properties. As a result, this complexation was shown to induce the static fluorescence quenching of MP and increase the thermal stability and antioxidant activity of C3G while decreasing its susceptibility to ascorbic acid, sucrose, and Fe-induced degradation and increasing its bioavailability during simulated in vitro digestion.

Proteomic analyses revealed the antibacterial mechanism of isolated anthocyanins against O157: H7.

anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, the antibacterial mechanism of AMAs against O157: H7 remains unclear. In this study, the tandem mass tag (TMT) quantitative proteomics was used to elucidate the potential antibacterial mechanisms of AMAs against O157: H7 cells.

Composition and antioxidant activity of anthocyanins from Aronia melanocarpa extracted using an ultrasonic-microwave-assisted natural deep eutectic solvent extraction method.

A time-saving, efficient, and environmentally friendly ultrasonic-microwave-assisted natural deep eutectic solvent (UMAE-NADES) extraction method was developed for the extraction of anthocyanins from Aronia melanocarpa. Eight different natural eutectic solvents were screened initially, and choline chloride-glycerol was selected as the extraction solvent. The extraction conditions were optimized using the response surface methodology, and the extraction rate of anthocyanins was higher than those achieved using the traditional ethanol method, natural deep eutectic solvent extraction method, and ultrasonic-microwave-assisted ethanol method.

Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.

This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment. Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice. The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.

Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.

The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure. The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods. The MP extracts recovery rate prepared by the thermal method was significantly higher (35.

Pharmacokinetic and excretion study of anthocyanins bound to amylopectin nanoparticles and their main metabolites using high-performance liquid chromatography-tandem mass spectrometry.

Anthocyanins of are known for their therapeutic properties; however, they are unstable and easily degrade in the environment and . Herein, we investigated the stability and bioavailability of four anthocyanins bound to amylopectin nanoparticles (APNPs) through a pharmacokinetic and excretion study using high-performance liquid chromatography-tandem mass spectrometry. An EC-C18 column with methanol and 0.

Gut Microbiota Modulation by Polyphenols from of LPS-Induced Liver Diseases in Rats.

polyphenols (AMPs) can alleviate the degree of liver diseases in rats. However, the mechanism by which this is achieved through gut microbiota modulation remains unclear. Here, a rich-polyphenol extract of (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats.

Preparing, optimising, and evaluating chitosan nanocapsules to improve the stability of anthocyanins from .

The anthocyanins extracted from are of great interest because of their potential health-related functionalities. However, their poor stabilities have limited their use in applications. Hence, to improve their physical and oxidative stability, we optimised the chitosan nanocapsules encapsulating anthocyanins from , and evaluated the systems in terms of their physicochemical characteristics, physical and oxidant stability in simulated gastrointestinal digestion and different storage environments.

Stability and structural characteristics of amylopectin nanoparticle-binding anthocyanins in Aronia melanocarpa.

The bioavailability of anthocyanins from Aronia melanocarpa is limited by their high degradation rates and poor stability. One way to protect anthocyanins is to deliver them using amylopectin nanoparticles (APNPs). In this study, we used distilled water as the reaction system, and achieved the most stable formulation of anthocyanins and APNPs using a mass ratio of 1:12, a pH of 3, and a binding time of 60 min, at which the binding rates were 81.