Evaluating the relationship between the proportion of X-chromosome deletions and clinical manifestations in children with turner syndrome.

Purpose: Analyze the relationship between changes in the proportion of X-chromosome deletions and clinical manifestations in children with Turner syndrome (TS).

Methods: X-chromosome number abnormalities in 8,635 children with growth retardation were identified using fluorescence hybridization (FISH). Meanwhile, the relationship between the proportion of X-chromosome deletions and the clinical manifestations of TS, such as face and body phenotype, cardiovascular, renal, and other comorbidities in children with TS was analyzed.

MEG3 polymorphisms associated with leukocyte telomere length in workers exposed to polycyclic aromatic hydrocarbons.

Long non-coding RNA maternally expressed gene 3 (MEG3) has been revealed to be involved in telomere length (TL) maintenance and homeostasis. However, it is unknown whether single-nucleotide polymorphisms (SNPs) in MEG3 could regulate TL in populations exposed to polycyclic aromatic hydrocarbons (PAHs). This study aimed to explore the effect of MEG3 genetic polymorphisms on TL in PAH-exposed populations.

[Application of fluorescence in situ hybridization combined with chromosomal karyotyping analysis in children with disorders of sex development due to sex chromosome abnormalities].

Objective: To retrospectively analyze sex chromosomal abnormalities and clinical manifestations of children with disorders of sex development (DSD).

Methods: A total of 14 857 children with clinical features of DSD including short stature, cryptorchidism, hypospadia, buried penis and developmental delay were recruited from Zhengzhou Children's Hospital from January 2013 to March 2022. Fluorescence in situ hybridization (FISH) and chromosomal karyotyping were carried out for such children.

Potential effects of the nursing work environment on the work-family conflict in operating room nurses.

Background: The nursing working environment is an important subsystem in the hospital environment. A good working environment could have a positive impact on nurses. However, the work-family conflict and unsatisfactory working environment could significantly reduce their working enthusiasm, efficacy as well as the overall quality of the nursing, increase their fatigue, and thereby compromise their career status.

[Application of multiple MLL gene rearrangement detection techniques for children with acute mononuclear leukemia].

Objective: To assess the value of detecting multiple rearrangements of MLL gene in children with acute mononuclear leukemia (AML).

Methods: Eighty six children with AML were analyzed by fluorescence in situ hybridization (FISH), chromosomal karyotyping and multiplex reverse transcription-PCR (RT-PCR).

Results: Cross signals were detected by FISH in 26 cases, and 30.

[The value of detecting MLL gene rearrangement in children with acute monocytic leukemia].

Objective: To assess the value of detecting the rearrangement of mixed lineage leukemia (MLL) gene in children with acute mononuclear leukemia (AML).

Methods: Dual-color fluorescence in situ hybridization (FISH) probe was used to detect MLL gene rearrangement in 68 children with AML by interphase FISH. The results were compared with that of conventional G banding chromosomal analysis.

Factor Analysis of Low-Frequency Repetitive Transcranial Magnetic Stimulation to the Temporoparietal Junction for Tinnitus.

. We investigated factors that contribute to suppression of tinnitus after repetitive transcranial magnetic stimulation (rTMS). .

Discrepancy in impact of maternal milk on vertical transmission between Hepatitis B virus and Human cytomegalovirus.

Objectives: This study aims to elucidate the role of breastfeeding on vertical transmission of HCMV and HBV and to investigate the difference in perinatal transmission via breast milk between HBV and HCMV.

Methods: This detailed study monitored the kinetics of viral DNA load in maternal milk for both HBV and HCMV, demonstrated the rate of transmission to infants, and compared HBV infection rate with that of HCMV.

Results: There was no difference in overall DNAlactia+ between HBV (23.