Nucleic Acids Res
December 2024
Programmable control of bacterial gene expression holds great significance for both applied and academic research. This is particularly true for Streptomyces, a genus of Gram-positive bacteria and major producers of prodigious natural products. Despite that a few inducible regulatory systems have been developed for use in Streptomyces, there is an increasing pursuit to augment the toolkit of high-performance induction systems.
View Article and Find Full Text PDFCuS has been identified as a functional material of memristors with multilevel resistance switching. However, as the migration of Cu ions under the electric field is tangled with defect evolution and phase transition, the electroresistance mechanism of CuS remains largely unclear. Here, the electrically triggered phase transition was studied by transmission electron microscopy.
View Article and Find Full Text PDFLuminescent silver clusters that are confined inside porous zeolites have attracted great research interest due to the combined advantages of high quantum yield, tunable emission, excellent chemical stability, and desirable adoption ability of this micronano-composite. In this research, a series of Ag-exchanged R-LTA/Ag (R = Li, Na, K) zeolites were synthesized, in which the silver clusters showed blue-shifted excitation in the UV region, red-shifted emission in the visible region, and decreased emission intensity when taking Li, Na, and K as the extra-framework cations, respectively. The [Ag(HO)R-sod] ( = 2, 4; R = Li, Na, K) structures were constructed, and the TD-DFT method was further carried out to study the influence of extra-framework cations on the luminescence performance of [Ag] clusters, in which the substitution of Al for Si and the reasonable hydrate levels were both taken into consideration.
View Article and Find Full Text PDFJiaogulan herbal tea, known for its pharmacological benefits, is derived from various species within the genus Gynostemma, particular for G. pentaphyllum (GP) and G. longipes (GL).
View Article and Find Full Text PDFClin Cosmet Investig Dermatol
November 2024
Red light optogenetic systems are in high demand for the precise control of gene expression for gene- and cell-based therapies. Here, we report a red/far-red light-inducible photoswitch (REDLIP) system based on the chimeric photosensory protein FnBphP (Fn-REDLIP) or PnBphP (Pn-REDLIP) and their interaction partner LDB3, which enables efficient dynamic regulation of gene expression with a timescale of seconds without exogenous administration of a chromophore in mammals. We use the REDLIP system to establish the REDLIP-mediated CRISPR-dCas9 (REDLIP) system, enabling optogenetic activation of endogenous target genes in mammalian cells and mice.
View Article and Find Full Text PDFJ Agric Food Chem
November 2024
Tryptophan not only serves as a fundamental building block for protein synthesis but also acts as a metabolic precursor for a diverse array of high-value chemicals. Although a few tryptophan-responsive biosensors are currently available, there is a growing interest in developing high-performance biosensors. In this study, we create a miniature toolkit of tryptophan biosensors based upon the leader regulatory region of the operon, which is responsible for tryptophan catabolism in .
View Article and Find Full Text PDFBioactive protein-derived hydrogels are highly attractive three-dimensional (3D) platforms for cell culture. However, most protein and polypeptide hydrogels are extracted from animal tissues or chemically synthesized, with many drawbacks. Herein, we fabricated an optically transparent ZmT-PEG hydrogel a facile one-pot strategy.
View Article and Find Full Text PDFFine-tuning gene expression is of great interest for synthetic biotechnological applications. This is particularly true for the genus , which is well-known as a prolific producer of diverse natural products. Currently, there is an increasing demand to develop effective gene induction systems.
View Article and Find Full Text PDFThe newly emerged Mn-based selenides as cathodes for aqueous Zn-ion batteries (ZIBs) have drawn researchers' interest because of their lower electronegativity and better electronic conductivity compared with the corresponding Mn-based oxides. Nevertheless, the energy storage mechanism of Mn-based selenides still needs to be further clarified. Herein, the MnSe/Se and MnSe polyhedral microspheres are reported as cathodes for ZIBs, and the MnSe cathode achieves significantly enhanced specific capacity, rate performance, and cycling stability.
View Article and Find Full Text PDFThe emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) has become a global health challenge due to the overuse of antibiotics. Natural substances including enzymes and essential oils have shown great potential as alternative treatment options. However, the combinational use of these natural agents remains challenging due to the denaturation of enzymes upon direct contact with oil.
View Article and Find Full Text PDFInt J Nanomedicine
August 2024
Phytochemical investigation of led to the purification of five novel dammarane-type triterpene isolates, gypenosides B1 - B5 (-). Their structures were determined through comprehensive 1D and 2D NMR spectroscopic analyses and HRESIMS data. Of note, are inseparable mixtures of epimers due to their unstable nature, and a total of eight dammarane-type triterpene saponins were identified.
View Article and Find Full Text PDFLow activation performance is a critical issue limiting the practical application of low-cost biochar in the advanced oxidation. Given the high potential of transition metals in the persulfate activation process and abundant oxygen-containing groups of hydrochar, hydrochar derived from cobalt (Co)-modified iron (Fe)-enriched sludge was synthesized and its performance and activation mechanism for the degradation of triclosan were investigated. Co modification significantly altered the morphology of hydrochar, and the increased Co-Fe mass ratios transformed hydrochar from granular to rose-shaped lamellar and then to helical sheet structures.
View Article and Find Full Text PDFLignin-based carbon material can be utilized as carbonaceous adsorbents for the removal of toxic gaseous organic pollutants, while the poor heat-resistance limited its widely application. Here in, B-N co-doped lignin carbon (BN-C) with high thermal stability was synthesized, and the optimized BN-C (1:2) exhibited notably improved heat resistance with the decomposition temperature up to 505 °C, and excellent adsorption capacity for o-dichlorobenzene (o-DCB) (1510.0 mg/g) and toluene (947.
View Article and Find Full Text PDFFufang Yinhua Jiedu granules (FYJG) is a Traditional Chinese Medicine (TCM) compound formulae preparation comprising ten herbal drugs, which has been widely used for the treatment of influenza with wind-heat type and upper respiratory tract infections. However, the phytochemical constituents of FYJG have rarely been reported, and its constituent composition still needs to be elucidated. The complexity of the natural ingredients of TCMs and the diversity of preparations are the major obstacles to fully characterizing their constituents.
View Article and Find Full Text PDFBackground: With the appearance and aggravation of the aging society, cancer has become one of the major problems that threaten the life and health of Chinese residents seriously.
Objective: To explore the cancer epidemiological characteristics among the elderly in China from 2005 to 2016, and to provide strategies for cancer prevention and treatment.
Methods: Stratified analysis was conducted on the cancer mortality data of the elderly aged ≥60 years in China, which were selected from the Chinese Cancer Registry Annual Report.
Schizophrenia symptomatology includes negative symptoms and cognitive impairment. Several studies have linked schizophrenia with the PDE4 family of enzymes due to their genetic association and function in cognitive processes such as long-term potentiation. We conducted a systematic gene expression meta-analysis of four PDE4 genes (PDE4A-D) in 10 brain sample datasets (437 samples) and three blood sample datasets (300 samples).
View Article and Find Full Text PDFSchizophrenia affects approximately 1% of the world population. Genetics, epigenetics, and environmental factors are known to play a role in this psychiatric disorder. While there is a high concordance in monozygotic twins, about half of twin pairs are discordant for schizophrenia.
View Article and Find Full Text PDFA copper-catalyzed highly -selective electrophilic aromatic alkylation of monosubstituted simple arenes has been accomplished. This method provides a practical platform for the transformation from simple commercial arenes to well-defined di- and multisubstituted aromatics with high added value. Control experiments and DFT calculations reveal that the achievement of the excellent site-selectivity is ascribed to the controlled deprotonation of the Wheland intermediates.
View Article and Find Full Text PDFPhotodynamic therapy (PDT) is a non-invasive, effective treatment for superficial skin conditions, offering superior cosmetic outcomes compared with traditional therapies. Bowen's disease (BD) of the nipple-areola complex (NAC) is rare and thus, lacks a standardized treatment approach. This report details the case of a 48-year-old woman who was successfully treated for BD of the NAC using PDT.
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