The impact of two different thaw protocols on outcomes of vitrified cleavage-stage embryos transfer.

The objective of this study was to evaluate the gestational results obtained with vitrified-thawed human cleavage-stage embryo by two different thaw protocols. Embryo development was observed to cleavage-stage and embryos were cryopreserved by vitrification on day 3 after oocyte retrieval. 51 cycles were thawed using vitrification warming kit with decreasing concentrations of sucrose in 3 dilutions ( 1.

Successful pregnancy following the transfer of vitrified blastocyst which developed from poor quality embryos on day 3.

Background: The selection of pre-embryos for transferred is based on morphological appearance. But some poor quality cleaved embryos also can be cultured to the blastocyst stage and implanted.

Objective: To assess the clinical pregnancy outcomes of blastocyst transfer which developed from poor quality embryos.

[Strain-induced tenogenic differentiation of bone marrow mesenchymal stem cells].

Objective: To study the possibility of bone marrow mesenchymal stem cells (BMSCs) differentiation into tenocytes (TCs) under strain stimulation by co-culture of BMSCs-small intestinal submucosa (SIS) composites in vitro.

Methods: BMSCs were isolated by adherent culture from the bone marrow of 1-week-old SD rats. Inducing method of multiple differentiation and flow cytometry were applied to identify the cells.

[Effect of vitreous-cryopreservation on in vivo implantation of tissue engineered tendons].

Objective: To study the immunological rejection occurred in different period after the in vivo implantation of vitreous-cryopreservation tissue engineered tendons for the repair of tendon defect and investigate its influences on the hepatic, renal, and cardiovascular function of rats.

Methods: Tenocytes obtained from tail tendon of one-week-old SD rats were cultured in vitro. The tenocytes at passage 2-4 (5 x 10(6) cells/mL) were co-cultured with 1.