Elucidating Binding Selectivity in Cyclin-Dependent Kinases 4, 6, and 9: Development of Highly Potent and Selective CDK4/9 Inhibitors.

CDK4/6 inhibitors are effective in treating HR/HER2 breast cancer but face limitations due to therapeutic resistance and hematological toxicity, particularly from strong CDK6 inhibition. To address these challenges, designing selective inhibitors targeting specific cyclin-dependent kinases (CDK) members could offer clinical advantages and broaden CDK inhibitor indications. However, the highly conserved binding pockets of CDKs complicate selective targeting.

Entrapment of bacterial cellulose nanocrystals stabilized Pickering emulsions droplets in alginate beads for hydrophobic drug delivery.

In this work, the interfacial assembly of amphiphilic bacterial cellulose nanocrystals (BCNs) by Pickering emulsion method was proposed to improve the compatibility between the alginate and hydrophobic drug. BCNs prepared by sulfuric acid hydrolysis of biosynthesized bacterial cellulose was used as the particulate emulsifiers, whereas the model drug, alfacalcidol, dissolved in CHCl was used as the oil phase. The oil-in-water Pickering emulsions were prepared by ultrasonic dispersion method and then they were well dispersed in alginate solution.

Synthesis of a benzyl-grafted alginate derivative and its effect on the colloidal stability of nanosized titanium dioxide aqueous suspensions for Pickering emulsions.

TiO nanoparticles (nano-TiO) as one of the most extensively used nanoscale materials easily undergo spontaneous aggregation and gravity sedimentation ascribed to their high adsorption energy, which significantly restricts their actual applications. For this reason, a benzyl-grafted alginate derivative (BAD) with good colloidal interface activity, prepared by a bimolecular nucleophilic substitution (S2) reaction, was used as the dispersant to stabilize nano-TiO. The structure and colloidal properties of BAD was evaluated by FT-IR spectroscopy, H NMR spectroscopy, thermal gravimetric analysis (TGA) and dynamic light scattering (DLS).