Exploring the Interplay of Wavelength, Quantum Yield, and Penetration Depth in In Vivo Fluorescence Imaging.

The intricate interplay between the irradiation wavelength, the fluorophore quantum yield (QY) and penetration depth profoundly influences the efficacy of in vivo fluorescence imaging in various applications. Understanding the complex behavior of fluorescence in vivo, specifically how variations in wavelength affect the QY of commonly used dyes and the depth of imaging is crucial for optimizing fluorescence imaging techniques, as it directly impacts the accuracy and efficiency of imaging in biological tissues. In our study, we explore these dynamics through Monte Carlo simulations conducted under conditions reflective of wide-field fluorescence imaging, examining how variations in wavelength impact the dye's QY and depth of imaging, and consequently, the fluorescence behavior.

Multiplexed near infrared fluorescence lifetime imaging in turbid media.

Significance: Fluorescence lifetime imaging (FLI) plays a pivotal role in enhancing our understanding of biological systems, providing a valuable tool for non-invasive exploration of biomolecular and cellular dynamics, both and . Its ability to selectively target and multiplex various entities, alongside heightened sensitivity and specificity, offers rapid and cost-effective insights.

Aim: Our aim is to investigate the multiplexing capabilities of near-infrared (NIR) FLI within a scattering medium that mimics biological tissues.

Non-monotonic dynamics of thin film spreading.

The phenomenon of a precursor spreading in front of an advancing droplet is still not fully understood. We recently used a driven lattice-gas model to study the microscopic dynamics of thin film spreading. We found that the scaling exponents describing the dynamics of both the precursor and the bulk layers are not universal, and strongly depend on the parameters describing the various interactions in the system.

Effect of temperature on the dynamics and geometry of reactive-wetting interfaces around room temperature.

The temperature effect on the dynamics and geometry of a mercury droplet (∼150 μm) spreading on a silver substrate (4000 Å) was studied. The system temperature was controlled by a heating stage in the temperature range of -15 °C < T < 25 °C, and the spreading process was monitored using an optical microscope. We studied the wetting dynamics (droplet radius and velocity) as a function of temperature.