Homeostatic Microfiber-Composed Synthetic Leathers with Chemical Robustness and Undercooling Self-Regulation.

Homeostatic microfiber leathers with temperature self-regulation promise broad applications, ranging from the apparel and automotive interior industries to the home furnishing and healthcare industries. Temperature self-regulation is achieved by phase-change materials containing microcapsules introduced within the leathers. The introduction of hase hange icrocapsules (PCMs) into microfiber leather faces two formidable challenges: (1) the shell of microcapsules must remain chemically stable against alkalis and organic solvents; (2) PCMs possess the ability to reduce supercooling during which latent heat is released, allowing for the precise and controllable release of latent heat.

Simultaneous detection of assembly and disassembly of multivalent HA tag and anti-HA antibody in single in-capillary assay.

Herein, we have developed an in-capillary assay for simultaneous detection of the assembly and disassembly of the multivalent HA tag peptide and antibody. HA tag with hexahistidine at C terminus (YPYDVPDYAG4 H6 , termed YPYDH6 ) was conjugated with quantum dots (QDs) by metal-affinity force to form a multivalent HA tag (QD-YPYDH6 ). QD-YPYDH6 and monoclonal anti-HA antibody (anti-HA) were sequentially injected into the capillary.

Simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection.

A novel assay was developed for the simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection. Quantum dots and PreScission protease were injected into a capillary sequentially, then mixed and assembled via a thioether bond upon coupling to glutathione S-transferase tag inside the capillary. The in-capillary assembly was influenced by the molar ratio and the time interval of injection.