High-risk ARGs (HRA) Chip: A high-throughput qPCR-based array for assessment of high-risk ARGs from the environment.

The global surge in antibiotic resistance genes (ARGs) presents a serious public health challenge. While methods like metagenomic analysis and qPCR arrays have been instrumental in investigating ARG distributions and dynamics, the vast diversity of ARGs often complicates effective monitoring and risk assessment. Here, we developed a High-Risk ARGs (HRA) chip based on high-capacity quantitative PCR array targeting previously identified high-risk ARGs.

Metabolomic Profiling Reveals the Quality Variations in Pericarpium ( Blanco cv. Chachiensis) with Different Storage Ages in Response to " Liberibacter Asiaticus" Infection.

Pericarpium, especially the pericarp of Blanco cv. Chachiensis (PCRC), is an important edible and medicinal ingredient for health and pharmacological properties. Citrus Huanglongbing, a devastating disease that currently threatens the citrus industry worldwide, is caused by a phloem-limited alpha-proteobacterium, " Liberibacter asiaticus" (CLas).

Integrated bacterial transcriptome and host metabolome analysis reveals insights into " Liberibacter asiaticus" population dynamics in the fruit pith of three citrus cultivars with different tolerance.

" Liberibacter asiaticus" (CLas), the causal agent of citrus Huanglongbing (HLB), is able to multiply to a high abundance in citrus fruit pith. However, little is known about the biological processes and phytochemical substances that are vital for CLas colonization and growth in fruit pith. In this study, CLas-infected fruit pith of three citrus cultivars ("Shatangju" mandarin, "Guanxi" pomelo, and "Shatian" pomelo) exhibiting different tolerance to CLas were collected and used for dual RNA-Seq and untargeted metabolome analysis.

Report of Citrus tristeza virus in Diaphorina citri (Hemiotera: Liviidae) insects of different sexes, color morphs, and developmental stages.

Diaphorina citri, also known as the Asian citrus psyllid, is the main vector of 'Candidatus Liberibacter asiaticus' (CLas) associated with citrus Huanglongbing. It has been reported that D. citri could also be infected by Citrus tristeza virus (CTV), a virus that has been previously reported to be vectored by certain aphid species.

Antibacterial photodynamic properties of silver nanoparticles-loaded curcumin composite material in chitosan-based films.

In order to cope with the increasingly severe food contamination and safety problems, a powerful sterilization of food packaging material is urgently needed. Chitosan (CS) has potential applications in food packaging due to its good film-forming properties, but its antibacterial activity is not sufficient to meet the needs in practical applications. Silver nanoparticles (AgNPs) have the problem of weak immediate antibacterial activity as a broad-spectrum antibacterial agent.

Variation of endosymbiont and (CTV) titers in the Huanglongbing insect vector, on CTV-infected plants.

" Liberibacter asiaticus" (CLas) is a notorious agent that causes Citrus Huanglongbing (HLB), which is transmitted by (). We recently found that the acquisition and transmission of CLas by was facilitated by (CTV), a widely distributed virus in the field. In this study, we further studied whether different CTV strains manipulate the host preference of , and whether endosymbionts variation is related to CTV strains in .

Local environment, surface characteristics and stochastic processes shape the dynamics of urban dustbin surface microbiome.

Dustbins function as critical infrastructures for urban sanitation, creating a distinct breeding ground for microbial assemblages. However, there is no information regarding the dynamics of microbial communities and the underlying mechanism for community assembly on dustbin surfaces. Here, surface samples were collected from three sampling zones (business building, commercial street and residential community) with different types (kitchen waste, harmful waste, recyclables, and others) and materials (metallic and plastic); and distribution pattern and assembly of microbial communities were investigated by high-throughput sequencing.

Promotes the Acquisition and Transmission of ' Liberibacter Asiaticus' by .

Kuwayama () is an insect vector of phloem-limited ' Liberibacter asiatus' (CLas), the presumed pathogen of citrus Huanglongbing (HLB). Recently, our lab has preliminarily found it acquired and transmitted (CTV), which was previously suggested to be vectored by species of aphids. However, the influences of one of the pathogens on the acquisition and transmission efficiency of the other pathogen remain unknown.

Current Epidemic Situation and Control Status of Citrus Huanglongbing in Guangdong China: The Space-Time Pattern Analysis of Specific Orchards.

Huanglongbing (HLB) is the most harmful bacterial disease in citrus production in the world, and has been seriously ravaging the citrus groves of South China since the 1930s. The surveillance of the epidemiological characteristics of HLB is of utmost priority for citrus production in this region. In order to explore the effects of disease control measures, analyses on the space-time statistical features of the HLB epidemic, from 2019 to 2021, within six orchards in the Guangdong province are presented.

Dynamics of Microbial Community and Potential Microbial Pollutants in Shopping Malls.

Shopping malls offer various niches for microbial populations, potentially serving as sources and reservoirs for the spread of microorganisms of public health concern. However, knowledge about the microbiome and the distribution of human pathogens in malls is largely unknown. Here, we examine the microbial community dynamics and genotypes of potential pathogens from floor and escalator surfaces in shopping malls and adjacent road dusts and greenbelt soils.

Integrated Transcriptome and Metabolome Analysis Reveals Phenylpropanoid Biosynthesis and Phytohormone Signaling Contribute to " Liberibacter asiaticus" Accumulation in Citrus Fruit Piths (Fluffy Albedo).

" Liberibacter asiaticus" (CLas) is a phloem-restricted α-proteobacterium that is associated with citrus huanglongbing (HLB), which is the most destructive disease that affects all varieties of citrus. Although midrib is usually used as a material for CLas detection, we recently found that the bacterium was enriched in fruits, especially in the fruit pith. However, no study has revealed the molecular basis of these two parts in responding to CLas infection.

Physiological Variables Influenced by ' Liberibacter asiaticus' Infection in Two Citrus Species.

' Liberibacter asiaticus' is the bacterium associated with the citrus disease known as huanglongbing (HLB). This study evaluated the influence of ' L. asiaticus' infection on a number of key plant physiological variables concerning photosynthesis, cell integrity, reactive oxygen species scavengers' activity, and osmoregulation of two different species of citrus-the pomelo and the mandarin 'Tankan'-relative to their measured ' L.

Uncovering the diversity and contents of gene cassettes in class 1 integrons from the endophytes of raw vegetables.

Rapid spread of antibiotic resistance genes (ARGs) in pathogens is threatening human health. Integrons allow bacteria to integrate and express foreign genes, facilitating horizontal transfer of ARGs in environments. Consumption of raw vegetables represents a pathway for human exposure to environmental ARGs.

Gallic acid functionalized chitosan immobilized nanosilver for modified chitosan/Poly (vinyl alcohol) composite film.

Food spoilage caused by bacterial growth is a serious threat to human health. Silver nanoparticles (AgNPs) have antibacterial activity against various pathogenic microorganisms, but their rapid release often leads to cumulative toxicity. In this paper, silver nanoparticles (AgNPs@CG) were reduced and immobilized in situ using gallic acid-functionalized chitosan (CG) as a reducing and stabilizing agent to achieve a long-lasting and stable controlled release of Ag.

Nanopore sequencing analysis of integron gene cassettes in sewages and soils.

Integrons are genetic elements that can facilitate rapid spread of antibiotic resistance by insertion and removal of genes. However, knowledge about the diversity and distribution of gene cassettes embedded in class 1 integron is still limited. In this study, we sequenced integron gene cassettes using nanopore sequencing and quantified antibiotic resistance genes (ARGs) and integrase genes in the manured soils and sewages of a bioreactor.

A Novel Phage (CLasMV1) From " Liberibacter asiaticus".

" Liberibacter asiaticus" (CLas) is an unculturable phloem-limited α-proteobacterium associated with citrus Huanglongbing (HLB; yellow shoot disease). HLB is currently threatening citrus production worldwide. Understanding the CLas biology is critical for HLB management.

Expanding control of the tumor cell cycle with a CDK2/4/6 inhibitor.

The CDK4/6 inhibitor, palbociclib (PAL), significantly improves progression-free survival in HR/HER2 breast cancer when combined with anti-hormonals. We sought to discover PAL resistance mechanisms in preclinical models and through analysis of clinical transcriptome specimens, which coalesced on induction of MYC oncogene and Cyclin E/CDK2 activity. We propose that targeting the G kinases CDK2, CDK4, and CDK6 with a small-molecule overcomes resistance to CDK4/6 inhibition.

Preliminary Report on the Acquisition, Persistence, and Potential Transmission of by .

(CTV) is one of the most important citrus tree viruses: a graft-transmissible virus that can be vectored by several aphid species. is the insect vector of " Liberibacter spp.", a bacterium associated with citrus Huanglongbing (HLB).

The Clinical Significance of Serum IL-33 and sST2 Alterations in the Post-Stroke Depression.

Introduction: This study was to test whether the serum levels of IL-33 and sST2 are correlated with the development of depression after acute ischemic stroke.

Methods: Patients diagnosed with acute ischemic stroke were selected. This study took the 24-item Hamilton Depression Rating Scale (HAMD) (score ≥20) as the diagnostic criteria for depression.

A Significantly High Abundance of " Liberibacter asiaticus" in Citrus Fruit Pith: Transcriptome and Anatomical Analyses.

Huanglongbing, a highly destructive disease of citrus, is associated with the non-culturable phloem-limited α-proteobacterium " Liberibacter asiaticus" (CLas). The distribution patterns of CLas in infected plant are variable and not consistent, which make the CLas detection and characterization more challenging. Here, we performed a systemic analysis of CLas distribution in citrus branches and fruits of 14 cultivars.

Discovery of PF-06873600, a CDK2/4/6 Inhibitor for the Treatment of Cancer.

Control of the cell cycle through selective pharmacological inhibition of CDK4/6 has proven beneficial in the treatment of breast cancer. Extending this level of control to additional cell cycle CDK isoforms represents an opportunity to expand to additional tumor types and potentially provide benefits to patients that develop tumors resistant to selective CDK4/6 inhibitors. However, broad-spectrum CDK inhibitors have a long history of failure due to safety concerns.

Spatial-temporal changes of forests and agricultural lands in Malaysia from 1990 to 2017.

Forests and agricultural lands are the main resources on the earth's surface and important indicators of regional ecological environments. In this paper, Landsat images from 1990 and 2017 were used to extract information on forests in Malaysia based on a remote-sensing classification method. The spatial-temporal changes of forests and agricultural lands in Malaysia between 1990 and 2017 were analyzed.