A Y-Shaped Peptide-Based Antifouling Electrochemical Aptasensor for Sensitive Aflatoxin B1 Detection in Food.

Conquering surface fouling of sensors caused by nonspecific adsorption and accumulation of foulants in a food matrix is of significance in accurate food safety analysis. Herein, an antifouling electrochemical aptasensor based on a Y-shaped peptide and nanoporous gold (NPG) for aflatoxin B1 detection in milk, tofu, and rice flour was proposed. The self-designed Y-shaped peptide involves an anchoring segment (-C), a support structure (-PPPP-), and an antifouling domain with two branches (-EK(KSRE)DER-) inspired by two bioactive peptides.

An antifouling electrochemical aptasensor based on a polydopamine-polyzwitterion copolymer for tetracycline analysis.

Matrix interference resulting from the nonspecific adsorption of non-target components, particularly proteins (fouling), onto sensor surfaces poses a persistent challenge in electrochemical detection of food hazards. The development of antifouling sensor surfaces presents a viable approach to mitigate nonspecific adsorption. In this study, a novel antifouling electrochemical aptasensor, utilizing a zwitterionic polymer, was developed for the sensitive, accurate, and selective detection of tetracycline (TC) in milk.