Derivation of species-specific hybridization-like knowledge out of cross-species hybridization results.

Background: One of the approaches for conducting genomics research in organisms without extant microarray platforms is to profile their expression patterns by using Cross-Species Hybridization (CSH). Several different studies using spotted microarray and CSH produced contradicting conclusions in the ability of CSH to reflect biological processes described by species-specific hybridization (SSH).

Results: We used a tomato-spotted cDNA microarray to examine the ability of CSH to reflect SSH data.