NSUN2 knockdown inhibits macrophage infiltration in diabetic nephropathy via reducing N5-methylcytosine methylation of SOCS1.

Objective: N5-methylcytosine (m5C) methylation is involved in various disease progression; however, its role in diabetic nephropathy (DN) has not been studied. The aim of this study was to investigate the role of NSUN2 in DN and the underlying mechanism.

Methods: Streptozotocin-induced experimental mouse model was generated to analyze the role of NSUN2 in vivo, and high glucose (HG)-treated Raw264.

Preparation of Polyoxymethylene/Exfoliated Molybdenum Disulfide Nanocomposite through Solid-State Shear Milling.

In this paper, the solid-state shear milling (SM) strategy featuring a very strong three-dimensional shear stress field was adopted to prepare the high-performance polyoxymethylene (POM)/molybdenum disulfide (MoS) functional nanocomposite. The transmission electron microscope and Raman measurement results confirmed that the bulk MoS particle was successfully exfoliated into few-layer MoS nanoplatelets by the above simple SM physical method. The polarized optical microscope (PLM) observation indicated the pan-milled nanoscale MoS particles presented a better dispersion performance in the POM matrix.

Mo C/Reduced Graphene Oxide Composites with Enhanced Electrocatalytic Activity and Biocompatibility for Microbial Fuel Cells.

A simple, cost-effective strategy was developed to effectively improve the electron transfer efficiency as well as the power output of microbial fuel cells (MFCs) by decorating the commercial carbon paper (CP) anode with an advanced Mo C/reduced graphene oxide (Mo C/RGO) composite. Benefiting from the synergistic effects of the superior electrocatalytic activity of Mo C, the high surface area, and prominent conductivity of RGO, the MFC equipped with this Mo C/RGO composite yielded a remarkable output power density of 1747±37.6 mW m , which was considerably higher than that of CP-MFC (926.

[Targeting Notch1 Gene Inhibits the Proliferation of Multiple Myeloma Cells].

Objective: To investigate the effects of Notch1 gene silencing on the proliferation and apoptosis of multiple myeloma cells, and to find the new targets for the treatment of multiple myeloma.

Methods: Notch1-shRNA targeted silencing Notch1 gene was transfected into multiple myeloma RPMI8226 cells, the CCK-8 and flow cytometry were used to detect the proliferation and apoptosis of myeloma cells after Notch1-shRNA transfection, the real-time fluorescence quantitative PCR was used to analyze expression level of Notch1 mRNA, and the Western blot were used to detect the expression level of Notch1 signaling pathway-related protein, such as Hes-1, Jagged-1, Jagged-2, BCL-2, PTEN, AKT and P-AKT.

Results: The mRNA and protein expression levels of Notch1-shRNA transfected cells were significantly inhibited in the experimental group assayed by real time fluorescence quantitative PCR and Western blot, the mRNA and protein expression level were down-regulated to 66% + 0.

[Notch1 Gene Silenced by RNAi Inhibits Oncogenicity of Myeloma Cell Line].

Objective: To investigate the effect of Notch1 gene silencing by RNA interference on oncogenicity of multiple myeloma cells in NOD/SCID mice.

Methods: Targeting-silenced Notch1 gene was target-sotenced by transfection of Notch1-shRNA in multiple myeloma RPMI8226 cells of NOD/SCID mouse myeloma models, and the change of the volume and speed of oncogenicity in myeloma mouse models were evaluated after Notch1 gene silencing, and ELISA was used to detect the serum expression level of IL-6 and VEGF in the tumor-bearing mice.

Results: After Notch1 gene was silenced by Notch1-shRNA, the speed of tumor formation was significantly inhibited and the tumor volume was reduced in the tumor-bearing mice, as compared with the control group, and the difference was statistically significant (P<0.

The Facile Synthesis of Branch-Trunk Ag Hierarchical Nanostructures and Their Applications for High-Performance H₂O₂ Electrochemical Sensors.

A novel branch-trunk Ag hierarchical nanostructure was synthesized via a galvanic replacement reaction combined with microwave-assisted synthesis using Te nanowire as a sacrificial template. The Te nanowire was synthesized via a hydrothermal process. We further investigated the potential application of the obtained hierarchical nanostructures in electrochemical sensor analysis.

Solar-microbial hybrid device based on oxygen-deficient niobium pentoxide anodes for sustainable hydrogen production.

Hydrogen gas is emerging as an attractive fuel with high energy density for the direction of energy resources in the future. Designing integrated devices based on a photoelectrochemical (PEC) cell and a microbial fuel cell (MFC) represents a promising strategy to produce hydrogen fuel at a low price. In this work, we demonstrate a new solar-microbial (PEC-MFC) hybrid device based on the oxygen-deficient NbO nanoporous (NbO NPs) anodes for sustainable hydrogen generation without external bias for the first time.

Holey tungsten oxynitride nanowires: novel anodes efficiently integrate microbial chemical energy conversion and electrochemical energy storage.

Holey tungsten oxynitride nanowires with superior conductivity, good biocompatibility, and good stability achieve excellent performance as anodes for both asymmetric supercapacitors and microbial fuel cells. Moreover, an innovative system is devised based on these as-prepared tungsten oxynitride anodes, which can simultaneously realize both energy conversion from chemical to electric energy and its storage.