[Effects of mixed-tocopherols and eicosapentaenoic acid on oxidized LDL-induced oxidative damage and inflammatory cytokine secretion in human umbilical vein endothelial cells].

Objective: To observe the influence of either alone or combined mixed-tocopherols combined with eicosapentaenoic acid (EPA) and α-Tocopherol use on oxidized LDL (oxLDL) induced 8-hydroxy-2'-deoxyguanosine (8-OHDG) and interleukin-6 (IL-6) secretion by human umbilical vein endothelial cells (HUVECs) and to explore the potential mechanism.

Method: Cultured HUVECs in vitro were incubated with oxLDL, oxLDL + α-tocopherol, oxLDL + mixed-tocopherols, oxLDL + EPA, oxLDL + α-tocopherol + EPA, oxLDL + mixed-tocopherols + EPA for 24 hours, respectively. Secretion of 8-OHDG and IL-6 were detected by cell enzyme linked immunosorbent assay (ELISA).

Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo.

The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method.

Cyclin D1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo.

The report aims to investigate the relationship between the expression of cyclin D1 and Cyclooxgenase-2 (COX-2), thus to explore the molecular mechanisms of the antitumor efficacy of Celecoxib, a COX-2 inhibitor. Human ovarian SKOV-3 carcinoma cell xenograft-bearing mice were treated with Celecoxib by infusing gaster (i.g.

[Effects of n-3 polyunsaturated fatty acids on hemorheology and coagulation in atherosclerotic rats].

Objective: To investigate the effect of n-3 polyunsaturated fatty acids (n-3PUFAs) on the hemorheology and coagulation function of high-fat induced atherosclerotic rats and understand the underlying mechanism.

Methods: Twenty-four Wistar rats were assigned randomly into 3 groups: normal control, model and n-3PUFAs treatment (n = 8 in each). The rats in model and treatment groups were injected with a single dose of vitamin D(3) (600,000 U/kg) and fed with a high-fat diet.

A review on applications of chemiluminescence detection in food analysis.

There is an increasing interest by consumers for high quality food products with a specified composition. Suitable analytical techniques are needed for the quality control. Chemiluminescence (CL) detection has become quite a useful tool in the last years due to its simplicity, low cost and high sensitivity.

[The predictive value of Holter recordings to detect moderate-severe obstructive sleep apnea syndrome].

Objective: To evaluate the predictive value of Holter ECG recordings for patients with moderate-severe obstructive sleep apnea and hypopnea syndrome (OSAHS).

Methods: Holter recordings was performed in 76 patients who were diagnosed OSAHS by polysomnography (PSG) within one month from Jan. 2008 to July 2009 in our hospital.

Enhanced sulfur and coking tolerance of a mixed ion conductor for SOFCs: BaZr(0.1)Ce(0.7)Y(0.2-x)Yb(x)O(3-delta).

The anode materials that have been developed for solid oxide fuel cells (SOFCs) are vulnerable to deactivation by carbon buildup (coking) from hydrocarbon fuels or by sulfur contamination (poisoning). We report on a mixed ion conductor, BaZr(0.1)Ce(0.

[Postprandial changes of blood lipid after ordinary Chinese diet and the influencing factors thereof].

Objective: To investigate the postprandial changes of blood lipid after ordinary Chinese diet and the influencing factors thereof.

Methods: Peripheral venous blood samples were collected from 88 patients, 72 males and 16 females, aged (65 +/- 12), 53 with hypertension, 35 with coronary heart diseases, and 27 with diabetes, while fasting and 4 h after breakfast and lunch to measure the levels of lipoproteins, including total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A (ApoA), ApoB, lipoprotein (Lp) (a), oxidized LDL (ox-LDL). Homeostasis model assessment insulin resistance index (HOMA-IR) was calculated.

Singlet oxygen generated from the decomposition of peroxymonocarbonate and its observation with chemiluminescence method.

The decomposition of peroxymonocarbonate (HCO(4)(-)) has been investigated by flow-injection chemiluminescence (CL) method. An ultraweak CL was observed during mixing the bicarbonate and hydrogen peroxide solution in organic cosolvent. An appropriate amount of fluorescent organic compounds, such as dichlorofluorescein (DCF), was added to the HCO(4)(-) solution, a strong CL was recorded.

Chemiluminescence from the decomposition of peroxymonocarbonate catalyzed by gold nanoparticles.

In this work, an online preparation of peroxymonocarbonate was formed innovatively, which offered the reliable intermediate for further investigation. Gold colloids with nanoparticles of different sizes were found to enhance the chemiluminescence (CL) of the peroxymonocarbonate-eosin Y system, and the most intensive CL signals were obtained with 50 +/- 1 nm diameter gold nanoparticles. UV-visible adsorption spectra, fluorescence spectra, transmission electron microscopy images, electron paramagnetic resonance spin-trapping spectra, and mass spectra were obtained in order to study the CL enhancement mechanism.

Ordered ZnO nanorods synthesized by combustion chemical vapor deposition.

Aligned single-crystal ZnO rod arrays with diameters ranging from 50 nm to 300 nm and length up to 10 microm have been synthesized on silicon substrate under open-air conditions using a combustion chemical vapor deposition (CVD) method. The ZnO rods grew in the direction of (0001) with six [1120] peripheral surface planes. A single ZnO rod solid-state gas sensor fabricated on an interdigitated electrode pattern demonstrated prompt response to ethanol vapor at 400 degrees C.

Electronic and vibrational properties of nickel sulfides from first principles.

We report the results of first-principles calculations (generalized gradient approximation-Perdew Wang 1991) on the electronic and vibrational properties of several nickel sulfides that are observed on Ni-based anodes in solid oxide fuel cells (SOFCs) upon exposure to H2S contaminated fuels: heazlewoodite Ni3S2, millerite NiS, polydymite Ni3S4, and pyrite NiS2. The optimized lattice parameters of these sulfides are within 1% of the values determined from x-ray diffraction. The electronic structure analysis indicates that all Ni-S bonds are strongly covalent.

A theoretical study of surface reduction mechanisms of CeO(2)(111) and (110) by H(2).

Reaction mechanisms for the interactions between CeO(2)(111) and (110) surfaces are investigated using periodic density functional theory (DFT) calculations. Both standard DFT and DFT+U calculations to examine the effect of the localization of Ce 4f states on the redox chemistry of H(2)-CeO(2) interactions are described. For mechanistic studies, molecular and dissociative local minima are initially located by placing an H(2) molecule at various active sites of the CeO(2) surfaces.

Chemical reduction of three-dimensional silica micro-assemblies into microporous silicon replicas.

The carbothermal reduction of silica into silicon requires the use of temperatures well above the silicon melting point (> or =2,000 degrees C). Solid silicon has recently been generated directly from silica at much lower temperatures (< or =850 degrees C) via electrochemical reduction in molten salts. However, the silicon products of such electrochemical reduction did not retain the microscale morphology of the starting silica reactants.

Determination of riboflavin by enhancing the chemiluminescence intensity of peroxomonosulfate-cobalt(II) system.

A weak chemiluminescent (CL) emission was observed in the decomposition of peroxomonosulfate (HSO5-), which would be accelerated in the presence of trace amounts of cobalt (II). The mechanism was due to the production of singlet oxygen (1O2). Interestedly, riboflavin can enhance the CL and the CL intensity was strongly dependent on riboflavin concentration.

Characterization of O2-CeO2 interactions using in situ Raman spectroscopy and first-principle calculations.

Interactions between O(2) and CeO(2) are examined experimentally using in situ Raman spectroscopy and theoretically using density-functional slab-model calculations. Two distinct oxygen bands appear at 825 and 1131 cm(-1), corresponding to peroxo- and superoxo-like species, respectively, when partially reduced CeO(2) is exposed to 10 % O(2). Periodic density-functional theory (DFT) calculations aid the interpretation of spectroscopic observations and provide energetic and geometric information for the dioxygen species adsorbed on CeO(2).

DNA functionalized single-walled carbon nanotubes for electrochemical detection.

Single-walled carbon nanotubes (SWNTs) were effectively dispersed and functionalized by wrapping with single-stranded DNA (ssDNA). The ssDNA-SWNTs attach strongly on glass substrate and easily form a uniform film, making it possible for electrochemical analysis and sensing. The film was fabricated into a working electrode, which exhibited good electrochemical voltammetric properties, such as flat and wide potential window, well-defined quasi-reversible voltammetric responses, and quick electron transfer for a Fe(CN)6(3-)/Fe(CN)6(4) system, indicating that the ssDNA-SWNTs film should be a good analytical electrode for electrochemical detection or sensing.

Probing water interactions and vacancy production on gadolinia-doped ceria surfaces using electron stimulated desorption.

Polycrystalline gadolinia-doped ceria (GDC) surfaces were studied using low-energy (5-400 eV) electron stimulated desorption (ESD). H(+), O(+), and H(3)O(+) were the primary cationic desorption products with H(+) as the dominant channel. H(+), H(3)O(+), and O(+) have a 22 eV threshold followed by a yield change around 40 eV.

Highly efficient electron stimulated desorption of O+ from gadolinia-doped ceria surfaces.

Highly efficient electron stimulated desorption of O+ from gadolinia-doped ceria (GDC) surfaces annealed at 850 K in ultrahigh vacuum is observed and investigated. O+ desorption has a major threshold of approximately 40 eV and an intrinsic kinetic energy of approximately 5.6 eV.

Chemiluminescence energy transfer reaction for the on-line preparation of peroxymonocarbonate and Eu(II)-dipicolinate complex.

In this work, an on-line preparation of peroxymonocarbonate was formed innovatively, which offered a reliable intermediate for further investigation. The forming conditions of on-line peroxymonocarbonate ions were investigated in detail. Meanwhile, the energy transfer chemiluminescent reaction of peroxymonocarbonate and the Eu(II)-dipicolinate complex was studied.

On-line preparation of peroxymonocarbonate and its application for the study of energy transfer chemiluminescence to lanthanide inorganic coordinate complexes.

It has been shown that peroxymonocarbonate ion (HCO(4) (-)) is a potent oxidant. In this study, a flow-injection system was developed in order to prepare on-line HCO(4) (-) ion and the optimum conditions for the on-line preparation of HCO(4) (-) were studied in detail. We used 99% (13)C-enriched NaHCO(3) to examine peroxymonocarbonate by (13)C-NMR at 25 degrees C.

Intra- and intermolecular proton transfer in 1H(2H)-1,2,3-triazole based systems.

Hartree-Fock ab initio and density functional theory calculations suggest that intra- and intermolecular proton transfer between neutral and protonated triazole tautomers play a vital role in the observed dramatic proton conduction in triazole-based polymer electrolyte membranes.

Promotion of proton conduction in polymer electrolyte membranes by 1H-1,2,3-triazole.

We report 1H-1,2,3-triazole as an active group to dramatically enhance proton conduction in a polymer electrolyte membrane (PEM). The conductivities of a poly(4-vinyl-1H-1,2,3-triazole) membrane without any acidic dopants are about 105 times greater than those of poly(4-vinylimidazole) in dry air at 50-150 degrees C. Polymers with groups promoting proton conduction attached to the backbone have great potential to offer excellent mechanical properties and long-term stability.