Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
February 2024
Mycobacterium tuberculosis heat resistant antigen (MTB-HAg) is a polypeptide antigen released from Mycobacterium tuberculosis (MTB) H37Ra to the supernatant after being autoclaved at 121 DegreesCelsius for 20 minutes. γδ T cells are unconventional T cells widely distributed in non-lymphoid tissues. They secrete cytokines such as TNF-α and IFN-γ, and play an important role in the immune response against MTB infection.
View Article and Find Full Text PDFMainstream non-volatile memory (NVM) devices based on floating gate structures or phase-change/ferroelectric materials face inherent limitations that compromise their suitability for long-term data storage. To address this challenge, a novel memory device based on light-programmed lattice engineering of thin rhenium disulfide (ReS ) flakes is proposed. By inducing sulfur vacancies in the ReS channel through light illumination, the device's electrical conductivity is modified accordingly and multiple conductance states for data storage therefore are generated.
View Article and Find Full Text PDFTuberculosis is an important chronic and often fatal infectious disease mainly caused by the bacterium (Mtb). Mtb is one of the most successful pathogens that harbors several potential virulence factors not found in nonpathogenic mycobacteria. As the Mtb cell envelope is closely associated with its virulence and resistance, it is very important to understand the cell envelope for better treatment of causative pathogen.
View Article and Find Full Text PDFTwo-dimensional nanoporous membranes hold great promise for the design of state-of-the-art desalination architectures to alleviate the increasing global water scarcity. Herein, by employing molecular dynamics simulations, we demonstrate the great potential of two recently reported metal-organic frameworks (MOF) membranes, namely NiIT and NiAT, as efficient desalination membranes that reach super high water flux and high salt rejection. The desalination performance of the MOF membrane is highly tunable through controlling the membrane thickness from one layer to five layers.
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
September 2022
Objective To establish a THP-1 macrophage model infected by Mycobacterium smegmatis expressing green fluorescent protein (GFP), to quickly locate and visually detect Mycobacterium smegmatis, and to provide a tracer tool to identify the pathogenesis of tuberculosis and develop new tuberculosis vaccines. Methods The enhanced green fluorescent protein (EGFP) gene sequence was amplified by PCR using pEGFP-N1 plasmid as a template to obtain the coding gene of EGFP, and the amplified product was cloned into the vector pALACE to establish the recombinant plasmid pALACE-EGFP. Electroporation transformed the pALACE-EGFP into Mycobacterium smegmatis, and recombinant Mycobacterium smegmatis clones were screened by hygromycin resistance.
View Article and Find Full Text PDFStudies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of . However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this gene is not easily expressed in may be mainly due to the high content of G+C and the use of unique codons.
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