[Research progress on the effect of Mycobacterium tuberculosis heat-resistant antigen (MTB-HAg) on γδ T cells].

Mycobacterium tuberculosis heat resistant antigen (MTB-HAg) is a polypeptide antigen released from Mycobacterium tuberculosis (MTB) H37Ra to the supernatant after being autoclaved at 121 DegreesCelsius for 20 minutes. γδ T cells are unconventional T cells widely distributed in non-lymphoid tissues. They secrete cytokines such as TNF-α and IFN-γ, and play an important role in the immune response against MTB infection.

A Light-Programmed Rewritable Lattice-Mediated Multistate Memory for High-Density Data Storage.

Mainstream non-volatile memory (NVM) devices based on floating gate structures or phase-change/ferroelectric materials face inherent limitations that compromise their suitability for long-term data storage. To address this challenge, a novel memory device based on light-programmed lattice engineering of thin rhenium disulfide (ReS ) flakes is proposed. By inducing sulfur vacancies in the ReS channel through light illumination, the device's electrical conductivity is modified accordingly and multiple conductance states for data storage therefore are generated.

PE8 (Rv1040c) Promotes the Intracellular Survival of Recombinant Mycobacterium by Regulating Host Inflammatory Cytokines and Inhibiting Cell Late Apoptosis.

Tuberculosis is an important chronic and often fatal infectious disease mainly caused by the bacterium (Mtb). Mtb is one of the most successful pathogens that harbors several potential virulence factors not found in nonpathogenic mycobacteria. As the Mtb cell envelope is closely associated with its virulence and resistance, it is very important to understand the cell envelope for better treatment of causative pathogen.

Metal-organic frameworks for high performance desalination through thickness control and structural fine-tuning.

Two-dimensional nanoporous membranes hold great promise for the design of state-of-the-art desalination architectures to alleviate the increasing global water scarcity. Herein, by employing molecular dynamics simulations, we demonstrate the great potential of two recently reported metal-organic frameworks (MOF) membranes, namely NiIT and NiAT, as efficient desalination membranes that reach super high water flux and high salt rejection. The desalination performance of the MOF membrane is highly tunable through controlling the membrane thickness from one layer to five layers.

[Establishment of a THP-1 macrophage model infected by recombinant Mycobacterium smegmatis expressing green fluorescent protein].

Objective To establish a THP-1 macrophage model infected by Mycobacterium smegmatis expressing green fluorescent protein (GFP), to quickly locate and visually detect Mycobacterium smegmatis, and to provide a tracer tool to identify the pathogenesis of tuberculosis and develop new tuberculosis vaccines. Methods The enhanced green fluorescent protein (EGFP) gene sequence was amplified by PCR using pEGFP-N1 plasmid as a template to obtain the coding gene of EGFP, and the amplified product was cloned into the vector pALACE to establish the recombinant plasmid pALACE-EGFP. Electroporation transformed the pALACE-EGFP into Mycobacterium smegmatis, and recombinant Mycobacterium smegmatis clones were screened by hygromycin resistance.

Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from and Preparation of Its Polyclonal Antibody Protein.

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of . However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this gene is not easily expressed in may be mainly due to the high content of G+C and the use of unique codons.